Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32040
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 79 4e-16
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 65 5e-12
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 38 7e-04
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 27 2.2
SPBC1D7.03 |mug80||cyclin Clg1 |Schizosaccharomyces pombe|chr 2|... 27 2.2
SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 2.9
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 26 3.8
SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|... 25 5.1
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 5.1
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 25 6.7
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||... 25 6.7
SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 6.7
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 6.7
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 25 8.9
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 8.9
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 79.0 bits (186), Expect = 4e-16
Identities = 56/150 (37%), Positives = 78/150 (52%), Gaps = 4/150 (2%)
Frame = +1
Query: 64 TRDQEQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQ 237
T E+E WI + L L N + D L+ GVILC++ K A G+ + +E F
Sbjct: 2 TSQLEKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFV 59
Query: 238 LMENIQRF-QAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQL 414
MENI F A + VP +++FQT+DLFERRN QV +++ R K L
Sbjct: 60 QMENISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGL 119
Query: 415 GPKMADKNERTFTEEQLRAHNAELN-LQMG 501
GPK+A+K R F+ +Q R +N LQ G
Sbjct: 120 GPKLAEKKPRVFSAQQQREFREGVNSLQYG 149
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 65.3 bits (152), Expect = 5e-12
Identities = 35/98 (35%), Positives = 52/98 (53%), Gaps = 2/98 (2%)
Frame = +1
Query: 82 EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 255
E WI + LG L P +E LR+GV+L L K P + KI F+ +NI
Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105
Query: 256 RFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 369
+F I G+PE F+ D++E +N+P+V C++AL
Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 38.3 bits (85), Expect = 7e-04
Identities = 30/101 (29%), Positives = 45/101 (44%), Gaps = 5/101 (4%)
Frame = +1
Query: 82 EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 249
E W+ + N + D L +G +LC+LA K P + R +Q+ E
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125
Query: 250 -IQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 369
+ F + G+ F+T DL R NIP+V CL+AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 26.6 bits (56), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 245 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 123
S+++KL + DP LAN +++ SLS +Y P G
Sbjct: 33 SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73
>SPBC1D7.03 |mug80||cyclin Clg1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 461
Score = 26.6 bits (56), Expect = 2.2
Identities = 23/75 (30%), Positives = 30/75 (40%), Gaps = 1/75 (1%)
Frame = -2
Query: 248 FSMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKT-SEIQVRTSCSW 72
F W L +SW P + SL TSS A + SG KT S + T+
Sbjct: 372 FEQDW-LASMSWNLSPGPRGQKAWEWWSASYSLFTSSNATY-SGEQKTYSPTTLSTNAPP 429
Query: 71 SLVSGRHCVQAKXWF 27
S SGR C ++
Sbjct: 430 SPSSGRSCSNCNYYY 444
>SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 163
Score = 26.2 bits (55), Expect = 2.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 162 DPLQIGQQISSWISEKNPRERNQLPAHG 245
D LQ+ ++ SWI+EK E +L + G
Sbjct: 11 DSLQVDEKAKSWIAEKVEEELRRLESVG 38
>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 749
Score = 25.8 bits (54), Expect = 3.8
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = -2
Query: 182 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 93
L+N +TPS S S Y P +T +Q
Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718
>SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 623
Score = 25.4 bits (53), Expect = 5.1
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = -2
Query: 356 HNVTWGMLRLSKRSAVWN 303
HN WG R + +A+WN
Sbjct: 491 HNFVWGRFRELEHAAMWN 508
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 25.4 bits (53), Expect = 5.1
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = -2
Query: 182 LANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLVS 60
L++ I+ L ++ +PF S S +E++ RTS ++ + S
Sbjct: 26 LSSYTSISRELDYANQSPFSSNSFSPTELKARTSATFDVRS 66
>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +2
Query: 374 ELLKNIPSLQDLS*DLKWLIRTNVPSRKNNFGPTTLNLTSK 496
EL+ N PS D + L + + VP+ + FG + + +K
Sbjct: 564 ELIPNDPSDDDYAAALNYAVNEYVPTYRKTFGNDSKKIKNK 604
>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1101
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 221 EEPTSSSWRTFKGSKRQSRNTEYQKKKYSKPPIF 322
E+P++S W+T GS+ ++ +KK S P F
Sbjct: 185 EDPSNSYWKTVIGSREMFEDS--RKKTSSPSPSF 216
>SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 701
Score = 25.0 bits (52), Expect = 6.7
Identities = 19/68 (27%), Positives = 34/68 (50%)
Frame = -1
Query: 453 REGTFVLISHFRS*LRSCKLGMFLSNSSQSVQT*CYLGNVTPLKKIGGLEYFFFWYSVFL 274
+EGT L + ++S +++ +S V + N T +K +GGLE F + L
Sbjct: 376 KEGTEKLFALMEE-IKSVNDNRYITETSNKV-----MENFTEIKNLGGLE-FLRSEASSL 428
Query: 273 DCRLEPLN 250
DC ++ +N
Sbjct: 429 DCLIDKVN 436
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 25.0 bits (52), Expect = 6.7
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -1
Query: 276 LDCRLEPLNVLHELEVGSSLLDFFH 202
LD LEPL LHE S+ L FF+
Sbjct: 238 LDAYLEPLVHLHEYSRRSNTLFFFY 262
>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 778
Score = 24.6 bits (51), Expect = 8.9
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -2
Query: 305 NISSSGTPYFLIAAWNL*MFSMSWKLVPLSWIFFTDPGANLLA 177
N +G PY I AW + S+ + P+ ++ + G +LLA
Sbjct: 136 NYKDNGVPYVTIRAWPALLSSL---VPPVVFLIMKESGYDLLA 175
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 24.6 bits (51), Expect = 8.9
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = +3
Query: 156 RCDPLQIGQQISSWISEKNPRERNQLP 236
RC P Q G S W +E P + LP
Sbjct: 1617 RCSPKQSGATRSVWFNELGPLTLSFLP 1643
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,354,493
Number of Sequences: 5004
Number of extensions: 51487
Number of successful extensions: 174
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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