BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV32039 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger) fa... 29 1.9 At2g28320.1 68415.m03442 pleckstrin homology (PH) domain-contain... 27 5.7 At3g54800.1 68416.m06064 pleckstrin homology (PH) domain-contain... 27 7.5 At2g20390.1 68415.m02380 expressed protein 27 7.5 At5g20450.1 68418.m02431 expressed protein weak similarity to my... 27 9.9 At1g04390.1 68414.m00429 expressed protein 27 9.9 >At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 1280 Score = 29.1 bits (62), Expect = 1.9 Identities = 16/43 (37%), Positives = 22/43 (51%) Frame = +2 Query: 194 LNEKYIYIIEFHRCNCCVIFT*SLHSIKQSRFLCPYIFPYVCL 322 + EKY ++EF + CCVIF S + + F C Y P L Sbjct: 1025 MQEKY-QVMEFQQRKCCVIFELSSNLARVLEF-CTYAMPQAFL 1065 >At2g28320.1 68415.m03442 pleckstrin homology (PH) domain-containing protein / lipid-binding START domain-containing protein contains Pfam profiles PF01852: START domain, PF00169: PH domain Length = 737 Score = 27.5 bits (58), Expect = 5.7 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = -3 Query: 115 CFHLKLVIASIDGHIDVIH 59 CF+ V+ +DGH D+IH Sbjct: 235 CFYQGSVVEHLDGHTDIIH 253 >At3g54800.1 68416.m06064 pleckstrin homology (PH) domain-containing protein / lipid-binding START domain-containing protein contains Pfam profiles PF00169: Pleckstring homology (PH) domain, PF01852: Lipid-binding START domain Length = 733 Score = 27.1 bits (57), Expect = 7.5 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = -3 Query: 115 CFHLKLVIASIDGHIDVIH 59 CF+ V+ +DGH D+IH Sbjct: 241 CFYKGNVVEHLDGHTDIIH 259 >At2g20390.1 68415.m02380 expressed protein Length = 183 Score = 27.1 bits (57), Expect = 7.5 Identities = 15/33 (45%), Positives = 18/33 (54%), Gaps = 1/33 (3%) Frame = -3 Query: 190 NSRLIVRMKPHAKYC-FPFIKPQG*QCFHLKLV 95 N+ L V + H+ C FP I PQG HLK V Sbjct: 87 NASLAVSHQRHSVSCSFPVIGPQGTGILHLKAV 119 >At5g20450.1 68418.m02431 expressed protein weak similarity to myosin [Arabidopsis thaliana] GI:433663 Length = 341 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/58 (20%), Positives = 30/58 (51%), Gaps = 4/58 (6%) Frame = -3 Query: 223 LDYVYVFFVQMNSRLIVRMKPHAKYC----FPFIKPQG*QCFHLKLVIASIDGHIDVI 62 +D++ + M L + PH +YC +PF P+ + H + ++ S+ +++++ Sbjct: 275 IDFLQKIYGMMRDNLKKEILPHLEYCKQAPWPFSNPKE-RIVHWQRIVGSLRSYLNIM 331 >At1g04390.1 68414.m00429 expressed protein Length = 849 Score = 26.6 bits (56), Expect = 9.9 Identities = 9/26 (34%), Positives = 18/26 (69%) Frame = -3 Query: 223 LDYVYVFFVQMNSRLIVRMKPHAKYC 146 L++ Y +V++ S + ++KP AK+C Sbjct: 593 LEFAYSGYVEVESTTLKKLKPLAKHC 618 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,023,954 Number of Sequences: 28952 Number of extensions: 189595 Number of successful extensions: 391 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 388 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 391 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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