Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32028
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 97 2e-21
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 63 2e-11
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 63 2e-11
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 56 4e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 35 0.006
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.015
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 33 0.025
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 32 0.044
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 30 0.18
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 29 0.31
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 28 0.95
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 0.95
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 6.7
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 6.7
SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 25 8.9
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 8.9
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 25 8.9
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 8.9
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 96.7 bits (230), Expect = 2e-21
Identities = 44/146 (30%), Positives = 91/146 (62%)
Frame = +2
Query: 62 MMNKRMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSXLQALIDENDPENSGKI 241
+ ++++A R+AF +FD + G I ++ ++ ++GQ + LQ +I+E D + +G I
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65
Query: 242 NFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGXGYITTSTLKEILAALDDKLSNAD 421
+F F + + +++ D E +E++EAF+++D++G GYIT L +L +L ++LS +
Sbjct: 66 DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 422 LDGIIAEIDTDGS*TVDFDEFMEMMT 499
+ +I E DTDG ++++EF +++
Sbjct: 123 VADMIREADTDGDGVINYEEFSRVIS 148
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 63.3 bits (147), Expect = 2e-11
Identities = 37/145 (25%), Positives = 77/145 (53%)
Frame = +2
Query: 56 IPMMNKRMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSXLQALIDENDPENSG 235
+P ++ +++AF ++D K G I + ++L ++G D+ L L +E
Sbjct: 1 MPASKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GD 56
Query: 236 KINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGXGYITTSTLKEILAALDDKLSN 415
I+ F + S+ L E ++E +E +AFR++D++ GYI T+ + + L +KLS+
Sbjct: 57 AIDEKKFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSD 113
Query: 416 ADLDGIIAEIDTDGS*TVDFDEFME 490
++ ++ E D S + D+ +F++
Sbjct: 114 NEVQLMVQEADPTNSGSFDYYDFVQ 138
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 63.3 bits (147), Expect = 2e-11
Identities = 32/137 (23%), Positives = 69/137 (50%)
Frame = +2
Query: 86 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSXLQALIDENDPENSGKINFDGFCNI 265
+ +AF++FD+ K ID ++ + +G + S + ++ + D G + + F +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 266 ASHFLEEEDAEAMQQELKEAFRLYDREGXGYITTSTLKEILAALDDKLSNADLDGIIAEI 445
+ + E D +E+K AF L+D + G I+ L+ + L++ + + +L+ +I E
Sbjct: 99 MTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF 155
Query: 446 DTDGS*TVDFDEFMEMM 496
D D ++ EF+ +M
Sbjct: 156 DLDQDGEINEQEFIAIM 172
Score = 37.5 bits (83), Expect = 0.001
Identities = 19/74 (25%), Positives = 37/74 (50%)
Frame = +2
Query: 278 LEEEDAEAMQQELKEAFRLYDREGXGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDG 457
L E E +Q++ EAF+L+D + I L+ + AL +++ I+ + D G
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86
Query: 458 S*TVDFDEFMEMMT 499
+ ++F+ +MT
Sbjct: 87 KGYLQMEDFVRVMT 100
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 55.6 bits (128), Expect = 4e-09
Identities = 43/151 (28%), Positives = 75/151 (49%), Gaps = 6/151 (3%)
Frame = +2
Query: 68 NKRMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSXLQALIDENDPENSGKINF 247
N+ + +RK F D +SG ID + +I + S L +++DE + G ++F
Sbjct: 20 NEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDE---DGGGDVDF 75
Query: 248 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGXGYITTSTLKEILAAL------DDKL 409
F N S F + E ++LK AF++YD + GYI+ L +L + +D+L
Sbjct: 76 QEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQL 132
Query: 410 SNADLDGIIAEIDTDGS*TVDFDEFMEMMTG 502
+D I E+D D + F+EF ++++G
Sbjct: 133 QQI-VDKTIMEVDKDRDGKISFEEFKDIVSG 162
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.1 bits (77), Expect = 0.006
Identities = 17/47 (36%), Positives = 27/47 (57%)
Frame = +2
Query: 308 QELKEAFRLYDREGXGYITTSTLKEILAALDDKLSNADLDGIIAEID 448
QELKEAF L D++G G I +K +L +L+ S ++ + I+
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 33.9 bits (74), Expect = 0.015
Identities = 21/64 (32%), Positives = 32/64 (50%)
Frame = +2
Query: 302 MQQELKEAFRLYDREGXGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGS*TVDFDE 481
M +E +EAF L+D GYI L+ A L + L+ L ++ T+G V +E
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREE 67
Query: 482 FMEM 493
F E+
Sbjct: 68 FAEL 71
Score = 31.5 bits (68), Expect = 0.077
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = +2
Query: 92 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSXLQALIDENDPENSGKINFDGFCNIAS 271
+AF +FD T GYID + +G+ LQ ++D +GK++ + F +
Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAELWI 73
Query: 272 H 274
H
Sbjct: 74 H 74
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 33.1 bits (72), Expect = 0.025
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = +2
Query: 317 KEAFRLYDREGXGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGS*TVDFDEFMEMM 496
K+AF L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 32.3 bits (70), Expect = 0.044
Identities = 26/105 (24%), Positives = 47/105 (44%), Gaps = 10/105 (9%)
Frame = +2
Query: 80 AMLRKAFQMFDTTKSGYIDVLKISTILN--TMGQLFDDSXLQALIDENDPENSGKINFDG 253
A F +FD K+GYID + L+ + G+L +D + A D +N+G I++D
Sbjct: 63 AFAEYVFNVFDADKNGYIDFKEFICALSVTSRGEL-NDKLIWA-FQLYDLDNNGLISYDE 120
Query: 254 FCNIASHFLE--------EEDAEAMQQELKEAFRLYDREGXGYIT 364
I + ED + ++ + + F + D+ G +T
Sbjct: 121 MLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLT 165
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 30.3 bits (65), Expect = 0.18
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +2
Query: 140 LKISTILNTMGQLFDDSXLQALIDENDPENSGKINFDGFCNIASHF 277
+ +ST+ +T + DDS L+ +ID+ PE GK NI++HF
Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 29.5 bits (63), Expect = 0.31
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 284 EEDAEAMQQELKEAFRLYDREGXGYITTSTLKE 382
++D M Q LKE Y+ E IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 27.9 bits (59), Expect = 0.95
Identities = 23/57 (40%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +2
Query: 320 EAFRLYDREGXGYITTSTLKEILAALDDKLSNADLDGI--IAEIDTDGS*TVDFDEF 484
E FR + E GY++ S +L + KLS+ L+ I +A+ID DG DFDEF
Sbjct: 11 EIFRGLNPEN-GYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGM--FDFDEF 62
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.9 bits (59), Expect = 0.95
Identities = 14/48 (29%), Positives = 24/48 (50%)
Frame = +2
Query: 224 ENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGXGYITT 367
ENS N D +A+ FL+E + E ++E + + L ++ I T
Sbjct: 435 ENSADTNDDIVNQVAAQFLDEREFETDEEEEESQYLLSTKDAINAINT 482
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.0 bits (52), Expect = 6.7
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +2
Query: 341 REGXGYITTSTLKEILAALDDKLSNADLDGIIAEID 448
RE GY+ T + D L+NA+L I+A+ID
Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142
>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 25.0 bits (52), Expect = 6.7
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +2
Query: 242 NFDGFCNIASHFLEEEDAEAM 304
+ DGF ++ASHF AE++
Sbjct: 269 SIDGFVSVASHFSSASTAESL 289
>SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 24.6 bits (51), Expect = 8.9
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +2
Query: 212 ENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFR 331
E D EN ++ A LE+ED EAM +L+E R
Sbjct: 152 EIDKENLAVLSQKSVLKTAEE-LEKEDREAMSAKLQELIR 190
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 24.6 bits (51), Expect = 8.9
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 209 DENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDR 343
D N+PE+ GF +I S +E D E+ + +YDR
Sbjct: 645 DCNNPESFSLFVDAGFISITSQNVETNDKESNSESETLYHLIYDR 689
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 24.6 bits (51), Expect = 8.9
Identities = 12/52 (23%), Positives = 23/52 (44%)
Frame = -3
Query: 184 IEQLSHCVENGGDLQHVDVTRLRGVKHLECFTQHGHPFVHHRNPMLHVKDWR 29
IE+ + + + D V + + L F ++ H+RNP+ DW+
Sbjct: 1587 IEETNAQIADVEDTLGATVKKSAPLDKLRGFMKYQRRSEHYRNPLKRTNDWK 1638
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1183
Score = 24.6 bits (51), Expect = 8.9
Identities = 11/36 (30%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +2
Query: 332 LYDREGXGYITT-STLKEILAALDDKLSNADLDGII 436
LYD+E GY+T + E++ + D+ N D + ++
Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVV 1090
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 24.6 bits (51), Expect = 8.9
Identities = 15/79 (18%), Positives = 32/79 (40%)
Frame = +2
Query: 23 LQPPILDVQHGIPMMNKRMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSXLQA 202
+Q P + V + ++ + + F D GY+ + + + + L
Sbjct: 242 VQAPNIPVSDPWAIPSQDLTSFCQLFSNVDKAHKGYVSGSEAYSFF--LASKLPEDVLAQ 299
Query: 203 LIDENDPENSGKINFDGFC 259
+ D +D ++GK+N FC
Sbjct: 300 IWDLSDTNSNGKLNIGEFC 318
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,939,156
Number of Sequences: 5004
Number of extensions: 36432
Number of successful extensions: 129
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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