Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV32008
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    80   2e-16
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    80   2e-16
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    54   1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    41   1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    40   2e-04
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    27   1.7  
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c...    25   6.7  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 79.8 bits (188), Expect = 2e-16
 Identities = 44/110 (40%), Positives = 65/110 (59%), Gaps = 6/110 (5%)
 Frame = +3

Query: 204 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 371
           + +Q+AEF+EAF L D D+DG I  N+L     SLG+  +  EL +M+ E     +G I+
Sbjct: 7   TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66

Query: 372 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWG 515
           FT+ LT+ A +M   +D ++ V  AFK FD++G   I  E L H L + G
Sbjct: 67  FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLG 115



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%)
 Frame = +3

Query: 222 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLT 389
           E +EAF++ D D +G I   +L     SLG   S++E+ +M+ EA     G IN+ +   
Sbjct: 86  EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145

Query: 390 LFANR 404
           + +++
Sbjct: 146 VISSK 150


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 79.8 bits (188), Expect = 2e-16
 Identities = 44/121 (36%), Positives = 65/121 (53%), Gaps = 2/121 (1%)
 Frame = +3

Query: 159 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 338
           ++ AKR  S  F+  +  Q+ E KEAF L+D D DG IG+ D++    SL + ASE  ++
Sbjct: 28  AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87

Query: 339 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 512
            M    + PIN    LT   + +   S  +D ++ AF TFD  + GKI    +R AL + 
Sbjct: 88  HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146

Query: 513 G 515
           G
Sbjct: 147 G 147


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 32/106 (30%), Positives = 58/106 (54%), Gaps = 2/106 (1%)
 Frame = +3

Query: 204 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 383
           S++Q  E KEAF L D DKDG+I  + + +   SLG   ++ EL ++  E    I+  + 
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 384 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWG 515
           ++  +N++     E++  I AF+ FD++  G I++ +    + T G
Sbjct: 64  MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLG 108


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 40.7 bits (91), Expect = 1e-04
 Identities = 18/42 (42%), Positives = 26/42 (61%)
 Frame = +3

Query: 222 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 347
           EF + FQ+ D D  G+IG  +LR    SLG   S +E+DE++
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 39.9 bits (89), Expect = 2e-04
 Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 6/103 (5%)
 Frame = +3

Query: 204 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 371
           +++Q  +  EAF+L D DKD  I  ++LRA   +LG  A + E+ +++ +      G + 
Sbjct: 32  TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91

Query: 372 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLR 494
               + +   ++    D  + +  AF+ F  DE GKI    LR
Sbjct: 92  MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLR 133


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +3

Query: 222 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 347
           E +EAF L D    G I   DLR +   LG   ++++L  M+
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54


>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1274

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +3

Query: 213 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 326
           + A+FK+ F     DKD +  + +L A  + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.317    0.124    0.389 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,558,023
Number of Sequences: 5004
Number of extensions: 23534
Number of successful extensions: 103
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)