Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31965
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g24550.1 68416.m03083 protein kinase family protein contains ... 31 0.46
At1g13830.1 68414.m01623 beta-1,3-glucanase-related similar to b... 31 0.61
At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 30 1.1
At2g40070.1 68415.m04923 expressed protein 29 1.4
At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin fa... 29 2.5
At3g22070.1 68416.m02785 proline-rich family protein contains pr... 28 3.2
At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 28 3.2
At3g28790.1 68416.m03593 expressed protein 27 5.7
At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family pro... 27 5.7
At1g06260.1 68414.m00662 cysteine proteinase, putative contains ... 27 5.7
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 27 7.5
At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138... 27 9.9
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 27 9.9
At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 27 9.9
At1g26150.1 68414.m03192 protein kinase family protein similar t... 27 9.9
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 31.1 bits (67), Expect = 0.46
Identities = 26/76 (34%), Positives = 32/76 (42%), Gaps = 6/76 (7%)
Frame = +1
Query: 19 PSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTP---WPSTPHRXMPIPEWVRNPAILP-- 183
PSS P L P S T P Q + SA +TP P+TP P + P P
Sbjct: 56 PSSPLPPSLPPPSPPGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGPPNTPSG 115
Query: 184 -IARAASNSVPKYPAD 228
R SN+ P P+D
Sbjct: 116 STPRTPSNTKPSPPSD 131
>At1g13830.1 68414.m01623 beta-1,3-glucanase-related similar to
beta-1,3-glucanase-like protein (GI:14279169) [Olea
europaea] similar to Glucan endo-1,3-beta-glucosidase
precursor (EC 3.2.1.39) ((1-3)-beta- glucan
endohydrolase) ((1-3)-beta-glucanase) (Beta-1,3-
endoglucanase) (Swiss-Prot:P52409) [Triticum aestivum]
Length = 197
Score = 30.7 bits (66), Expect = 0.61
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +1
Query: 49 PASTQ-PDSTQPFAQTTRSAMLTPWPSTPHRXMPIP 153
P+ST+ P ST P TT + TP+P TP P P
Sbjct: 110 PSSTRSPPSTTPPTGTTPTNGTTPFPGTPFPGTPFP 145
>At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catalytic
subunit, putative similar to SP|O48653 DNA polymerase
alpha catalytic subunit (EC 2.7.7.7) {Oryza sativa};
contains Pfam profiles: PF03175 DNA polymerase type B,
organellar and viral, PF00136 DNA polymerase family B,
PF03104 DNA polymerase family B, exonuclease domain
Length = 1492
Score = 29.9 bits (64), Expect = 1.1
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +3
Query: 81 ICPNYPFCDADALAKYT 131
+CPNYP C+ L KYT
Sbjct: 1407 VCPNYPNCNGTLLRKYT 1423
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 29.5 bits (63), Expect = 1.4
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 1/59 (1%)
Frame = +1
Query: 61 QPDSTQPFAQTTRSAMLTPW-PSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPADFP 234
+P ++ A TT + ++ PS+P P+P +NPA+ A S P P+D P
Sbjct: 319 RPIASASAATTTANPTISQIKPSSPAPAKPMPTPSKNPALSRAASPTVRSRPWKPSDMP 377
>At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin
family protein contains Pfam profile PF01190: Pollen
proteins Ole e I family
Length = 401
Score = 28.7 bits (61), Expect = 2.5
Identities = 26/84 (30%), Positives = 33/84 (39%), Gaps = 2/84 (2%)
Frame = +1
Query: 4 TESCSPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRX-MP-IPEWVRNPAI 177
T + P+ L P P S P P T + L P P TP +P IP P +
Sbjct: 283 TPTLPPNPLIPS---PPSLPPIPLIPTPPTLPTIPLLPTPPTPTLPPIPTIPTLPPLPVL 339
Query: 178 LPIARAASNSVPKYPADFPAALCP 249
P+ S+P P FP L P
Sbjct: 340 PPVPIVNPPSLPPPPPSFPVPLPP 363
>At3g22070.1 68416.m02785 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 178
Score = 28.3 bits (60), Expect = 3.2
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
Frame = +1
Query: 52 ASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPADF 231
+ST P S+ + ++ S +TP+P+ P P + + P ++S S+ P
Sbjct: 45 SSTDPSSSSSSSSSSTSPFITPFPNPNPNPNPNPPVLGSSPPSPTDSSSSTSISPNP--- 101
Query: 232 PAALC-PNYP 258
PA + PN P
Sbjct: 102 PAPIVNPNPP 111
>At1g62970.1 68414.m07110 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226: DnaJ domain
Length = 797
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 4/75 (5%)
Frame = +1
Query: 46 WPASTQPDSTQPFAQTTRSAMLTPWP----STPHRXMPIPEWVRNPAILPIARAASNSVP 213
+P S P +++PF + S P+P S + + + + + LP++++ NS P
Sbjct: 393 FPLSQPPSNSKPFPMSQSSQNSKPFPVSQSSQKSKPLLVSQSSQRSKPLPVSQSLQNSNP 452
Query: 214 KYPADFPAALCPNYP 258
+P P++ +P
Sbjct: 453 -FPVSQPSSNSKPFP 466
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/75 (22%), Positives = 39/75 (52%), Gaps = 4/75 (5%)
Frame = +1
Query: 46 WPASTQPDSTQPF--AQTTRSA--MLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVP 213
+P S +++PF +Q+++ + +L S + +P+ + ++N P+++ +SNS P
Sbjct: 405 FPMSQSSQNSKPFPVSQSSQKSKPLLVSQSSQRSKPLPVSQSLQNSNPFPVSQPSSNSKP 464
Query: 214 KYPADFPAALCPNYP 258
+P P +P
Sbjct: 465 -FPVSQPQPASNPFP 478
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/57 (29%), Positives = 21/57 (36%)
Frame = +1
Query: 55 STQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPA 225
+T D+T + + S TP PSTP P P P S P PA
Sbjct: 261 NTYKDTTGSSSGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPSTPAPSTPA 317
>At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family
protein low similarity to ubiquitin-conjugating
BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
contains Pfam profile PF00179: Ubiquitin-conjugating
enzyme
Length = 907
Score = 27.5 bits (58), Expect = 5.7
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = +3
Query: 87 PNYPFCDADALAKYTPQG----NADTRMGAQPSH 176
P++ FC +D + K P+G NADT + + H
Sbjct: 397 PDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKH 430
>At1g06260.1 68414.m00662 cysteine proteinase, putative contains
similarity to thiol-protease, pre-pro-TPE4A protein
GI:3688528 [Pisum sativum]
Length = 343
Score = 27.5 bits (58), Expect = 5.7
Identities = 18/73 (24%), Positives = 30/73 (41%)
Frame = -2
Query: 239 AAGKSAGYLGTEFDAARAIGRMAGLRTHSGIGIXLWGVLGQGVSIAERVVWANGWVESGW 60
+ G AG + ++ G + G+ + +GV G + W GW E G+
Sbjct: 259 SVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEEGY 318
Query: 59 VLAGQSYRGQSED 21
+ + RG SED
Sbjct: 319 I---RMERGVSED 328
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 27.1 bits (57), Expect = 7.5
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 1/82 (1%)
Frame = +1
Query: 10 SCSPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIA 189
S SPSS P L P+S P ++ S L+P P + P + + P++
Sbjct: 52 SLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSPPSSSPSSAPPSSLSPSSPPPLS 111
Query: 190 RAASNSVPKYPADFP-AALCPN 252
+ S+ P P+ P ++L P+
Sbjct: 112 LSPSSPPPPPPSSSPLSSLSPS 133
Score = 26.6 bits (56), Expect = 9.9
Identities = 25/83 (30%), Positives = 33/83 (39%)
Frame = +1
Query: 10 SCSPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIA 189
S SPSS P L P+S P S P + +P S P P P+ P +
Sbjct: 42 SSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSP-----PSSSPSS 96
Query: 190 RAASNSVPKYPADFPAALCPNYP 258
S+ P P P +L P+ P
Sbjct: 97 APPSSLSPSSPP--PLSLSPSSP 117
>At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'PsRT17-1 like protein' based on similarity to PsRT17-1
(GP:1778376) [Pisum sativum] which was based upon
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 519
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 137 LWGVLGQGVSIAERVVWANGWVESGWVLAGQSYRGQS 27
L G L + +SI ++V + + GW +SYRG S
Sbjct: 60 LQGALDRQISIRQQVELWSPLADQGWKPCTESYRGAS 96
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 26.6 bits (56), Expect = 9.9
Identities = 21/82 (25%), Positives = 28/82 (34%)
Frame = +1
Query: 16 SPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARA 195
SP P+ P + QP Q T +P P P + P PE P +
Sbjct: 533 SPKPQPPKQETPKPEESPKPQPPKQETPKPEESPKPQPPKQETPKPEESPKPQPPKQEQP 592
Query: 196 ASNSVPKYPADFPAALCPNYPY 261
PK + + PN PY
Sbjct: 593 PKTEAPKMGSPPLESPVPNDPY 614
>At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 665
Score = 26.6 bits (56), Expect = 9.9
Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 4/69 (5%)
Frame = +1
Query: 49 PAST-QPDSTQPFAQTTRSAMLTP--WPSTPHRXM-PIPEWVRNPAILPIARAASNSVPK 216
P+S +P+ P+ A TP P P R P P + +PA P + V K
Sbjct: 338 PSSVFRPERVSPWELEPLVANSTPSSQPQPPQRNKRPRPPGLPSPATGPSGPVTPDGVWK 397
Query: 217 YPADFPAAL 243
PAD P+++
Sbjct: 398 SPADTPSSV 406
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 26.6 bits (56), Expect = 9.9
Identities = 19/70 (27%), Positives = 28/70 (40%)
Frame = +1
Query: 49 PASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPAD 228
P T+ T P + P P +P +P P+ NP P S+S P++
Sbjct: 127 PPPTEAPPTTPITSPSPPTNPPPPPESPPS-LPAPDPPSNPLPPPKLVPPSHSPPRHLPS 185
Query: 229 FPAALCPNYP 258
PA+ P P
Sbjct: 186 PPASEIPPPP 195
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,869,675
Number of Sequences: 28952
Number of extensions: 285428
Number of successful extensions: 715
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 713
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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