Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31961
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    57   2e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    46   3e-06
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    43   2e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    29   0.31 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   0.95 
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   3.8  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 56.8 bits (131), Expect = 2e-09
 Identities = 29/75 (38%), Positives = 44/75 (58%)
 Frame = +1

Query: 28  AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 207
           A+K KD    E+  E  K++DK+ NG +   ELTH L +LGE+L   EVA++ ++     
Sbjct: 75  ARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132

Query: 208 DDDGMIPYAAFLKKV 252
           D DG+I Y  F + +
Sbjct: 133 DGDGVINYEEFSRVI 147



 Score = 29.1 bits (62), Expect = 0.41
 Identities = 15/61 (24%), Positives = 32/61 (52%)
 Frame = +1

Query: 61  DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 240
           +F E   L+D++++G +   EL   + +LG+    +E+ ++  +     D +G I +  F
Sbjct: 13  EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70

Query: 241 L 243
           L
Sbjct: 71  L 71


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 46.4 bits (105), Expect = 3e-06
 Identities = 26/86 (30%), Positives = 48/86 (55%)
 Frame = +1

Query: 1   EXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 180
           E FL + ++       G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E
Sbjct: 58  EQFLQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDE 117

Query: 181 VTKDCMDPEDDDGMIPYAAFLKKVMA 258
           + K        DGM+ Y  F++ ++A
Sbjct: 118 LLKGV---PVKDGMVNYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 43.2 bits (97), Expect = 2e-05
 Identities = 21/77 (27%), Positives = 46/77 (59%)
 Frame = +1

Query: 28  AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 207
           + K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP 
Sbjct: 68  SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP- 125

Query: 208 DDDGMIPYAAFLKKVMA 258
            + G   Y  F++++MA
Sbjct: 126 TNSGSFDYYDFVQRIMA 142



 Score = 30.7 bits (66), Expect = 0.14
 Identities = 14/60 (23%), Positives = 34/60 (56%)
 Frame = +1

Query: 58  EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 237
           ++  E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 29.5 bits (63), Expect = 0.31
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = +1

Query: 19  YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 192
           Y+  + +  +   +D  E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 24  YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81



 Score = 27.9 bits (59), Expect = 0.95
 Identities = 20/85 (23%), Positives = 38/85 (44%)
 Frame = +1

Query: 1   EXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 180
           E F+ + ++   ++D    E+     +L+D +E G +    L      L E +DD E+  
Sbjct: 93  EDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150

Query: 181 VTKDCMDPEDDDGMIPYAAFLKKVM 255
           + ++     D DG I    F+  +M
Sbjct: 151 MIEEF--DLDQDGEINEQEFIAIMM 173


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 0.95
 Identities = 13/31 (41%), Positives = 15/31 (48%)
 Frame = -2

Query: 407  APPEELSPPRALPAPVPQSRASVF*GPSHRT 315
            APP    PP A P P+P S A     P  R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +1

Query: 145 LGEKLDDSEVAEVTKDCMDPED 210
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,893,819
Number of Sequences: 5004
Number of extensions: 31850
Number of successful extensions: 97
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 97
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)