Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31955
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri... 149 8e-37
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic... 141 2e-34
At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast... 141 2e-34
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast... 141 2e-34
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 140 5e-34
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic... 121 3e-28
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic... 118 2e-27
At4g37235.1 68417.m05271 expressed protein 30 0.80
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 29 1.9
At1g75530.1 68414.m08778 forkhead-associated domain-containing p... 28 4.3
At4g39840.1 68417.m05645 expressed protein 27 9.9
At1g48640.1 68414.m05444 lysine and histidine specific transport... 27 9.9
>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
/ transaminase A (ASP1) identical to SP|P46643 Aspartate
aminotransferase, mitochondrial precursor (EC 2.6.1.1)
(Transaminase A) {Arabidopsis thaliana}
Length = 430
Score = 149 bits (362), Expect = 8e-37
Identities = 73/144 (50%), Positives = 95/144 (65%)
Frame = +3
Query: 75 GLRASSTWWNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE 254
GLR+ S+WW +V+ P D ILG+TEA+ D P+KVN+GVGAYRDD GKP VL VR+AE
Sbjct: 25 GLRSMSSWWKSVEPAPKDPILGVTEAFLADPSPEKVNVGVGAYRDDNGKPVVLECVREAE 84
Query: 255 EILHSRGLNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEF 434
+ L + EY P+ G A D KLA+G++S IK+K VQTLSGTGA RL +F
Sbjct: 85 KRL-AGSTFMEYLPMGGSAKMVDLTLKLAYGDNSEFIKDKRIAAVQTLSGTGACRLFADF 143
Query: 435 ITKHYAKAKEIWLPTPTWGNHPQI 506
K ++ +I++P PTW NH I
Sbjct: 144 -QKRFSPGSQIYIPVPTWSNHHNI 166
>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
isozyme 1 / transaminase A (ASP2) identical to SP|P46645
Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
Length = 405
Score = 141 bits (342), Expect = 2e-34
Identities = 70/139 (50%), Positives = 90/139 (64%), Gaps = 1/139 (0%)
Frame = +3
Query: 99 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRG 275
++NV P D ILG+T AY D P K+NLGVGAYR +EGKP VL VRKAE ++++
Sbjct: 5 FSNVARAPEDPILGVTVAYNNDPSPVKINLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPS 64
Query: 276 LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAK 455
EY PI G + + AKL G DSP I TVQ LSGTG+LR+G EF+ HY +
Sbjct: 65 RVKEYIPIVGISDFNKLSAKLILGADSPAITESRVTTVQCLSGTGSLRVGAEFLKTHYHQ 124
Query: 456 AKEIWLPTPTWGNHPQICN 512
+ I++P PTWGNHP++ N
Sbjct: 125 S-VIYIPKPTWGNHPKVFN 142
>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 141 bits (342), Expect = 2e-34
Identities = 68/138 (49%), Positives = 92/138 (66%)
Frame = +3
Query: 99 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 278
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 279 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 458
N EY PI G A + A A+L FG PVIK + T+Q LSGTG+LRL I +++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 459 KEIWLPTPTWGNHPQICN 512
K + + +PTWGNH I N
Sbjct: 173 KVV-ISSPTWGNHKNIFN 189
>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 141 bits (342), Expect = 2e-34
Identities = 68/138 (49%), Positives = 92/138 (66%)
Frame = +3
Query: 99 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGL 278
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 279 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 458
N EY PI G A + A A+L FG PVIK + T+Q LSGTG+LRL I +++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 459 KEIWLPTPTWGNHPQICN 512
K + + +PTWGNH I N
Sbjct: 173 KVV-ISSPTWGNHKNIFN 189
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 140 bits (339), Expect = 5e-34
Identities = 70/137 (51%), Positives = 91/137 (66%), Gaps = 1/137 (0%)
Frame = +3
Query: 99 WNNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRG 275
++++ P D ILG+T AY KD P K+NLGVGAYR +EGKP VL VRKAE ++++ R
Sbjct: 49 FSHLVQAPEDPILGVTVAYNKDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRT 108
Query: 276 LNHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAK 455
EY PI G + AKL G DSP I+ TV+ LSGTG+LR+G EF+ KHY +
Sbjct: 109 RIKEYLPIVGLVEFNKLSAKLILGADSPAIRENRITTVECLSGTGSLRVGGEFLAKHYHQ 168
Query: 456 AKEIWLPTPTWGNHPQI 506
K I++ PTWGNHP+I
Sbjct: 169 -KTIYITQPTWGNHPKI 184
>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 403
Score = 121 bits (292), Expect = 3e-28
Identities = 60/135 (44%), Positives = 82/135 (60%)
Frame = +3
Query: 102 NNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLN 281
++V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L + L+
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQL-ANDLD 64
Query: 282 HEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKAK 461
EY P++G + KL G+DSP +K T Q LSGTG+LR+G EF+ H K
Sbjct: 65 KEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATH-NKES 123
Query: 462 EIWLPTPTWGNHPQI 506
I++P PTWGNHP+I
Sbjct: 124 VIFVPNPTWGNHPRI 138
>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 405
Score = 118 bits (285), Expect = 2e-27
Identities = 59/136 (43%), Positives = 81/136 (59%), Gaps = 1/136 (0%)
Frame = +3
Query: 102 NNVQMGPPDVILGITEAYKKDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEIL-HSRGL 278
++V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L +
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQLANDLSR 65
Query: 279 NHEYAPISGEATYTDAVAKLAFGEDSPVIKNKSNCTVQTLSGTGALRLGLEFITKHYAKA 458
+ EY P++G + KL G+DSP +K T Q LSGTG+LR+G EF+ H K
Sbjct: 66 DKEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATH-NKE 124
Query: 459 KEIWLPTPTWGNHPQI 506
I++P PTWGNHP+I
Sbjct: 125 SVIFVPNPTWGNHPRI 140
>At4g37235.1 68417.m05271 expressed protein
Length = 152
Score = 30.3 bits (65), Expect = 0.80
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 4/44 (9%)
Frame = -2
Query: 401 GQCLYSTVALIF----DDRAVFSKSQFSYSISICGLTTDGSILM 282
GQ L+S+ +L+F DD ++ + F Y +++ GL T S+ +
Sbjct: 19 GQTLFSSASLLFMCFNDDEDFYAYTTFCYLVTVMGLVTPWSVTL 62
>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
domain-containing protein / F-box family protein similar
to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
pombe}; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00646: F-box domain
Length = 1378
Score = 29.1 bits (62), Expect = 1.9
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 360 VIKNKSNCTVQTLSGTGALRLGLEFITKHYAKAKEIW 470
+ +N ++C + G+GAL L L F+T H + IW
Sbjct: 1238 MFQNDADCMALLMDGSGALGLDLSFVT-HVFLMEPIW 1273
>At1g75530.1 68414.m08778 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 555
Score = 27.9 bits (59), Expect = 4.3
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +3
Query: 462 EIWLPTPTWGNHPQICN 512
EIW+P P + HP+I N
Sbjct: 231 EIWIPPPPYDPHPEIVN 247
>At4g39840.1 68417.m05645 expressed protein
Length = 451
Score = 26.6 bits (56), Expect = 9.9
Identities = 23/89 (25%), Positives = 34/89 (38%)
Frame = +3
Query: 12 LKKLTTQVLKPNNVDTIVGCTGLRASSTWWNNVQMGPPDVILGITEAYKKDTHPKKVNLG 191
LKKL + N+ +I L SS+ N + PP L + KK + P +
Sbjct: 141 LKKLNSGTKSTNSTSSIKKSADLSKSSSSKNKTTIKPPSSKLSSPPSEKK-SQPSSKPVT 199
Query: 192 VGAYRDDEGKPFVLPSVRKAEEILHSRGL 278
+ E KPF L + + R L
Sbjct: 200 KSKQSEKEIKPFWLDDEEDEDFVSEFRDL 228
>At1g48640.1 68414.m05444 lysine and histidine specific transporter,
putative similar to lysine and histidine specific
transporter GI:2576361 from [Arabidopsis thaliana];
contains Pfam profile PF01490: Transmembrane amino acid
transporter protein
Length = 453
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/55 (27%), Positives = 32/55 (58%)
Frame = -3
Query: 367 LMTGLSSPKASLATASVYVASPLMGAYS*LSPLLWRISSAFLTEGKTNGFPSSSL 203
++ L +P ++ATA+++V ++G+Y + ++ + FL + K N PS+ L
Sbjct: 305 VLMSLETPVWAIATANLFVVMHVIGSYQIFAMPVFDMVETFLVK-KLNFKPSTVL 358
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,401,540
Number of Sequences: 28952
Number of extensions: 275996
Number of successful extensions: 708
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 696
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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