Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31952
(505 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 33 0.14
At5g53400.1 68418.m06635 nuclear movement family protein contain... 31 0.33
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 31 0.33
At2g32820.1 68415.m04017 hypothetical protein and genscan 30 0.77
At5g45520.1 68418.m05591 hypothetical protein 29 1.3
At4g27500.1 68417.m03950 expressed protein non-consensus GA dono... 29 1.3
At4g25210.1 68417.m03628 expressed protein weak similarity to st... 28 3.1
At4g24010.1 68417.m03450 cellulose synthase family protein simil... 28 4.1
At3g13880.1 68416.m01754 pentatricopeptide (PPR) repeat-containi... 28 4.1
At3g24780.1 68416.m03110 hypothetical protein 27 5.4
At1g02205.2 68414.m00154 CER1 protein identical to maize gl1 hom... 27 5.4
At1g02205.1 68414.m00153 CER1 protein identical to maize gl1 hom... 27 5.4
At5g16630.1 68418.m01947 DNA repair protein Rad4 family low simi... 27 7.2
At2g43140.1 68415.m05357 basic helix-loop-helix (bHLH) family pr... 27 9.5
At2g18630.1 68415.m02169 expressed protein unusual splice site a... 27 9.5
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 32.7 bits (71), Expect = 0.14
Identities = 23/75 (30%), Positives = 38/75 (50%), Gaps = 2/75 (2%)
Frame = +1
Query: 40 VQKKVTQ-KPRIPDPSESTVHGKE-VHVAKQKQTQKEVVGDKEITRKITSTETTEIEHKA 213
V++K Q +P P + G+E AK+K+ +KE +KE + T+ +E K
Sbjct: 325 VEEKAAQPEPVAPVENAGEKEGEEETAAAKKKKKKKEK--EKEKKAAAAAAATSSVEVKE 382
Query: 214 QTQERVVEGPVKPSK 258
+ QE V P++P K
Sbjct: 383 EKQEESVTEPLQPKK 397
>At5g53400.1 68418.m06635 nuclear movement family protein contains
Pfam profile: PF03593 nuclear movement protein
Length = 304
Score = 31.5 bits (68), Expect = 0.33
Identities = 21/75 (28%), Positives = 38/75 (50%)
Frame = +1
Query: 34 QRVQKKVTQKPRIPDPSESTVHGKEVHVAKQKQTQKEVVGDKEITRKITSTETTEIEHKA 213
++ +KK +K + P E KE+ +K+ +KE V + T +S E E+E
Sbjct: 69 KKAEKKKAEKESVK-PVEKKAE-KEIVKLVEKKVEKESV---KPTIAASSAEPIEVEKPK 123
Query: 214 QTQERVVEGPVKPSK 258
+ +E+ GP+ P+K
Sbjct: 124 EEEEKKESGPIVPNK 138
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis
thaliana}
Length = 1473
Score = 31.5 bits (68), Expect = 0.33
Identities = 20/99 (20%), Positives = 39/99 (39%)
Frame = +1
Query: 34 QRVQKKVTQKPRIPDPSESTVHGKEVHVAKQKQTQKEVVGDKEITRKITSTETTEIEHKA 213
+++ K Q + + ++ + K K K+ + K+ T+K + +ETTE + A
Sbjct: 1171 EKLDLKDAQVQQAIEAAQKKIRAKSGAAVKVKRQAPKKPAPKKTTKKASESETTEASYSA 1230
Query: 214 QTQERVVEGPVKPSKPPIFTKKMQPCRVFEHEQARFEVE 330
+ V VKP KK E + + +
Sbjct: 1231 MDTDNNVAEVVKPKARQGAKKKASESETTEASHSAMDTD 1269
Score = 27.1 bits (57), Expect = 7.2
Identities = 27/96 (28%), Positives = 34/96 (35%), Gaps = 3/96 (3%)
Frame = +1
Query: 34 QRVQKKVTQKPRIPDPSESTVHGKEVHVAKQKQTQKEVVGDKE---ITRKITSTETTEIE 204
++ KK K SES EVV K +K + +ETTE
Sbjct: 1203 RQAPKKPAPKKTTKKASESETTEASYSAMDTDNNVAEVVKPKARQGAKKKASESETTEAS 1262
Query: 205 HKAQTQERVVEGPVKPSKPPIFTKKMQPCRVFEHEQ 312
H A + V VKP K KK P E E+
Sbjct: 1263 HSAMDTDNNVAEVVKP-KGRQGAKKKAPAAAKEVEE 1297
>At2g32820.1 68415.m04017 hypothetical protein and genscan
Length = 333
Score = 30.3 bits (65), Expect = 0.77
Identities = 19/83 (22%), Positives = 39/83 (46%), Gaps = 1/83 (1%)
Frame = +1
Query: 250 PSKPPIFTKKMQPCRVFEHEQARFEVEFDG-EPLPLIKWYRENFPIKNSPDFQIHTFSTK 426
PS+PP+ KK QP + FE+ + ++ + E L L + +++ + N+ F
Sbjct: 215 PSRPPLNMKKHQPVKAFENPKTCLVLKKNSPEMLELFEMAKKSADVANAKGFLAAKEEAS 274
Query: 427 SILIIRQVFVEDSAVFAAVAENR 495
+ I + ++ S + A+ R
Sbjct: 275 ICVDILALLMKFSIISTAIETRR 297
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 29.5 bits (63), Expect = 1.3
Identities = 22/73 (30%), Positives = 32/73 (43%)
Frame = +1
Query: 43 QKKVTQKPRIPDPSESTVHGKEVHVAKQKQTQKEVVGDKEITRKITSTETTEIEHKAQTQ 222
+KK + S+ V G++ K K +K+ GDKE + E+E K
Sbjct: 689 EKKQDEVEAEKSKSDEIVEGEKKPDDKSK-VEKKGDGDKENADLDEGKKRDEVEAKKSES 747
Query: 223 ERVVEGPVKPSKP 261
+VVEG K S P
Sbjct: 748 GKVVEGDGKESPP 760
>At4g27500.1 68417.m03950 expressed protein non-consensus GA donor
splice site at exon 6
Length = 612
Score = 29.5 bits (63), Expect = 1.3
Identities = 15/60 (25%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
Frame = +1
Query: 109 VHVAKQKQTQKEVVGDKEITRKITSTETTEIEHKAQTQERVVEGPV---KPSKPPIFTKK 279
+ K+ + +++ +K+ +K TE E + E +E PV KP K +F +K
Sbjct: 503 IRAQKEAEKKEKKEQEKKAKKKTGGNTETETEEVPEASEEEIEAPVQEEKPQKEKVFKEK 562
>At4g25210.1 68417.m03628 expressed protein weak similarity to
storekeeper protein [Solanum tuberosum] GI:14268476;
contains Pfam profile PF04504: Protein of unknown
function, DUF573
Length = 368
Score = 28.3 bits (60), Expect = 3.1
Identities = 16/74 (21%), Positives = 35/74 (47%), Gaps = 2/74 (2%)
Frame = +1
Query: 73 PDPSESTVHGKEVHVAKQKQ--TQKEVVGDKEITRKITSTETTEIEHKAQTQERVVEGPV 246
P +E V V +++ + +E E+ +K+ S++ E + + +++ GP
Sbjct: 3 PKKAEEVVESPPVSSEEEESGSSGEESESSAEVPKKVESSQKPESDSEGESESESSSGP- 61
Query: 247 KPSKPPIFTKKMQP 288
+P P T K++P
Sbjct: 62 EPESEPAKTIKLKP 75
>At4g24010.1 68417.m03450 cellulose synthase family protein similar
to Zea mays cellulose synthase-5 [gi:9622882], -4
[gi:9622880]
Length = 770
Score = 27.9 bits (59), Expect = 4.1
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 308 SCSNTRQGCIFLVNIGGFEGLTGPS 234
+C N R +F++N+ GF+GL GP+
Sbjct: 361 ACENKR---LFIINMVGFDGLMGPT 382
>At3g13880.1 68416.m01754 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 748
Score = 27.9 bits (59), Expect = 4.1
Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 4/37 (10%)
Frame = -3
Query: 401 KSGEFLIGKFSRYHLIKGKGSPS----NSTSNLACSC 303
KSG ++GK + H+IK +P N+ N+ C C
Sbjct: 59 KSGSVVLGKLAHGHMIKSSLNPCLYLLNNLLNMYCKC 95
>At3g24780.1 68416.m03110 hypothetical protein
Length = 715
Score = 27.5 bits (58), Expect = 5.4
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = +2
Query: 251 LRNHRYSLRKCSPAVCLNMNKLDSKLSSMANPCL 352
+R R L K SPA+ N+ + LSS NPCL
Sbjct: 96 VRRRRVDLNKSSPAMGYTENRSATYLSS-GNPCL 128
>At1g02205.2 68414.m00154 CER1 protein identical to maize gl1
homolog (glossy1 locus) GI:1209703 and CER1 GI:1199467
from [Arabidopsis thaliana]
Length = 625
Score = 27.5 bits (58), Expect = 5.4
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -2
Query: 267 YRWFRRFNWPFHN-SLLCLSFMLDLRCFSRSNF 172
YRWF R WPF + S++ F L R++F
Sbjct: 323 YRWFMRLLWPFTSLSMIFTLFYARLFVAERNSF 355
>At1g02205.1 68414.m00153 CER1 protein identical to maize gl1
homolog (glossy1 locus) GI:1209703 and CER1 GI:1199467
from [Arabidopsis thaliana]
Length = 580
Score = 27.5 bits (58), Expect = 5.4
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -2
Query: 267 YRWFRRFNWPFHN-SLLCLSFMLDLRCFSRSNF 172
YRWF R WPF + S++ F L R++F
Sbjct: 323 YRWFMRLLWPFTSLSMIFTLFYARLFVAERNSF 355
>At5g16630.1 68418.m01947 DNA repair protein Rad4 family low
similarity to SP|Q01831 DNA-repair protein complementing
XP-C cells (Xeroderma pigmentosum group C complementing
protein) {Homo sapiens}; contains Pfam profile PF03835:
DNA repair protein Rad4
Length = 865
Score = 27.1 bits (57), Expect = 7.2
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +1
Query: 349 PLIKWYRENFPIKNSP 396
PL++W RENF + SP
Sbjct: 192 PLLRWVRENFSVSCSP 207
>At2g43140.1 68415.m05357 basic helix-loop-helix (bHLH) family
protein contains Pfam profile PF00010: Helix-loop-helix
DNA-binding domain
Length = 295
Score = 26.6 bits (56), Expect = 9.5
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 504 SPTSIFSHSSKHSGIFDKHLSYD 436
S +S+F H S +G +D+HL D
Sbjct: 78 SSSSLFRHRSSPAGFYDQHLPTD 100
>At2g18630.1 68415.m02169 expressed protein unusual splice site at
second intron; GA instead of conserved GT at donor site;
similar to At14a GI:11994571 and GI:11994573
[Arabidopsis thaliana]
Length = 393
Score = 26.6 bits (56), Expect = 9.5
Identities = 20/55 (36%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = -2
Query: 468 SGIFDKHLSYDQYRLGTKC---MNLEVRRVFNREILPIPFNQRQGFAIELNFESS 313
SG+ K LS+D R T+C MN +V +V ++ I NQ + L FES+
Sbjct: 67 SGVEIKSLSFDSLREVTQCLLDMNQDVVKVILQDKEDIWNNQDLFSLVNLYFEST 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.314 0.130 0.372
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,605,678
Number of Sequences: 28952
Number of extensions: 220317
Number of successful extensions: 589
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 577
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 589
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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