Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31919
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26240.1 68418.m03129 chloride channel protein (CLC-d) identi... 79 1e-15
At3g27170.1 68416.m03398 chloride channel protein (CLC-b) identi... 79 2e-15
At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, pu... 71 5e-13
At5g40890.1 68418.m04965 chloride channel protein (CLC-a) identi... 68 3e-12
At5g49890.1 68418.m06178 chloride channel protein (CLC-c) identi... 66 1e-11
At2g25850.2 68415.m03104 nucleotidyltransferase family protein c... 29 1.9
At2g25850.1 68415.m03103 nucleotidyltransferase family protein c... 29 1.9
At5g45190.1 68418.m05547 cyclin family protein similar to cyclin... 28 3.2
At1g21695.1 68414.m02716 hydroxyproline-rich glycoprotein family... 28 3.2
At5g04620.2 68418.m00464 aminotransferase class I and II family ... 28 4.3
At4g32850.6 68417.m04675 nucleotidyltransferase family protein c... 28 4.3
At4g32850.5 68417.m04674 nucleotidyltransferase family protein c... 28 4.3
At4g32850.4 68417.m04671 nucleotidyltransferase family protein c... 28 4.3
At4g32850.3 68417.m04673 nucleotidyltransferase family protein c... 28 4.3
At4g32850.2 68417.m04672 nucleotidyltransferase family protein c... 28 4.3
At4g32850.1 68417.m04676 nucleotidyltransferase family protein c... 28 4.3
At4g00540.1 68417.m00074 myb family transcription factor 27 9.9
At2g34560.2 68415.m04246 katanin, putative similar to katanin p6... 27 9.9
At2g34560.1 68415.m04245 katanin, putative similar to katanin p6... 27 9.9
At1g73590.1 68414.m08519 auxin efflux carrier protein, putative ... 27 9.9
>At5g26240.1 68418.m03129 chloride channel protein (CLC-d) identical
to CLC-d chloride channel protein [Arabidopsis thaliana]
GI:1742959
Length = 792
Score = 79.4 bits (187), Expect = 1e-15
Identities = 42/143 (29%), Positives = 74/143 (51%), Gaps = 2/143 (1%)
Frame = +1
Query: 1 LQLYCEX-GEYNALAAIWFQTPEASVRS-FLHDPMGSYKPWSILVFVVCYFLLSTWTFGL 174
+ +C+ EYN LA I+F T + ++R+ F M + S+L F+ ++ L+ TFG
Sbjct: 409 VNFFCKTDNEYNDLATIFFNTQDDAIRNLFSAKTMREFSAQSLLTFLAMFYTLAVVTFGT 468
Query: 175 AVSSGLFIPNLLTGAAWGRLLAISIQYMLPSKTINPAKYXXXXXXXXXXXXXRMTISLTV 354
AV +G F+P ++ G+ +GRL+ + + I Y RMT+SL V
Sbjct: 469 AVPAGQFVPGIMIGSTYGRLVGMFVVRFYKKLNIEEGTYALLGAASFLGGSMRMTVSLCV 528
Query: 355 IIIETTGQISNALPIMITLVVAQ 423
I++E T + IM+ L++++
Sbjct: 529 IMVEITNNLKLLPLIMLVLLISK 551
Score = 36.7 bits (81), Expect = 0.009
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = +2
Query: 422 KWIGDFFNEGIYDIYIQLAGVPLLPWEP 505
K +GD FNEG+Y++ +L G+PLL P
Sbjct: 551 KAVGDAFNEGLYEVQARLKGIPLLESRP 578
>At3g27170.1 68416.m03398 chloride channel protein (CLC-b) identical
to CLC-b chloride channel protein GB:CAA96058 from
[Arabidopsis thaliana] (J. Biol. Chem. 271 (52),
33632-33638 (1996))
Length = 780
Score = 79.0 bits (186), Expect = 2e-15
Identities = 51/142 (35%), Positives = 76/142 (53%), Gaps = 2/142 (1%)
Frame = +1
Query: 4 QLYCEXGEYNALAAIWFQTPEASVRS-FLHDPMGSYKPWSILVFVVCYFLLSTWTFGLAV 180
Q +C G YN LA + T + +VR+ F + + S+ +F V Y +L +TFG+A
Sbjct: 415 QFHCPKGYYNDLATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIAT 474
Query: 181 SSGLFIPNLLTGAAWGRLLAISIQYMLPSKTINPAKYXXXXXXXXXXXXXRMTISLTVII 360
SGLF+P +L GAA+GR+L + M +I+ Y RMT+SL VI
Sbjct: 475 PSGLFLPIILMGAAYGRMLGAA---MGSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIF 531
Query: 361 IETTGQISNALPI-MITLVVAQ 423
+E T + LPI MI L++A+
Sbjct: 532 LELTNNLL-LLPITMIVLLIAK 552
Score = 35.9 bits (79), Expect = 0.016
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +2
Query: 422 KWIGDFFNEGIYDIYIQLAGVPLLPWEPXP 511
K +GD FN IYDI + L G+P L P P
Sbjct: 552 KTVGDSFNPSIYDIILHLKGLPFLEANPEP 581
>At5g33280.1 68418.m03944 chloride channel-like (CLC) protein,
putative similar to CLC-c, At5g49890 [Arabidopsis
thaliana] and chloride channel protein ClC-1 - Nicotiana
tabacum, PIR:T02939
Length = 763
Score = 70.9 bits (166), Expect = 5e-13
Identities = 38/138 (27%), Positives = 75/138 (54%), Gaps = 1/138 (0%)
Frame = +1
Query: 13 CEXGEYNALAAIWFQTPEASVRS-FLHDPMGSYKPWSILVFVVCYFLLSTWTFGLAVSSG 189
C G YN LA++ F T + ++++ F + + +S+LVF V F LS +++G+ +G
Sbjct: 401 CPPGHYNDLASLIFNTNDDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAG 460
Query: 190 LFIPNLLTGAAWGRLLAISIQYMLPSKTINPAKYXXXXXXXXXXXXXRMTISLTVIIIET 369
LF+P ++TGA++GR + + + + +N + RMT+S VI++E
Sbjct: 461 LFVPVIVTGASYGRFVGMLLG---SNSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLEL 517
Query: 370 TGQISNALPIMITLVVAQ 423
T + +M+ L++++
Sbjct: 518 TNNLLLLPMMMVVLLISK 535
>At5g40890.1 68418.m04965 chloride channel protein (CLC-a) identical
to GI:1742952 (gb|AAC05742.1)
Length = 775
Score = 68.1 bits (159), Expect = 3e-12
Identities = 47/142 (33%), Positives = 71/142 (50%), Gaps = 2/142 (1%)
Frame = +1
Query: 4 QLYCEXGEYNALAAIWFQTPEASVRS-FLHDPMGSYKPWSILVFVVCYFLLSTWTFGLAV 180
Q C G YN L+ + T + +VR+ F + + S+ +F Y +L TFG+A
Sbjct: 416 QFNCPNGYYNDLSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIAT 475
Query: 181 SSGLFIPNLLTGAAWGRLLAISIQYMLPSKTINPAKYXXXXXXXXXXXXXRMTISLTVII 360
SGLF+P +L G+A+GR+L + M I+ Y RMT+SL VI
Sbjct: 476 PSGLFLPIILMGSAYGRMLGTA---MGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIF 532
Query: 361 IETTGQISNALPI-MITLVVAQ 423
+E T + LPI M L++A+
Sbjct: 533 LELTNNLL-LLPITMFVLLIAK 553
Score = 33.5 bits (73), Expect = 0.086
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 422 KWIGDFFNEGIYDIYIQLAGVPLLPWEPXP 511
K +GD FN IY+I + L G+P L P P
Sbjct: 553 KTVGDSFNLSIYEIILHLKGLPFLEANPEP 582
>At5g49890.1 68418.m06178 chloride channel protein (CLC-c) identical
to gi:1742956
Length = 779
Score = 66.1 bits (154), Expect = 1e-11
Identities = 37/138 (26%), Positives = 71/138 (51%), Gaps = 1/138 (0%)
Frame = +1
Query: 13 CEXGEYNALAAIWFQTPEASVRS-FLHDPMGSYKPWSILVFVVCYFLLSTWTFGLAVSSG 189
C YN L+++ T + ++R+ F + ++ +F V + L T+G+A+ SG
Sbjct: 429 CPPNHYNDLSSLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSG 488
Query: 190 LFIPNLLTGAAWGRLLAISIQYMLPSKTINPAKYXXXXXXXXXXXXXRMTISLTVIIIET 369
LFIP +L GA++GRL+ + + P ++ + RMT+SL VI++E
Sbjct: 489 LFIPVILAGASYGRLVG---RLLGPVSQLDVGLFSLLGAASFLGGTMRMTVSLCVILLEL 545
Query: 370 TGQISNALPIMITLVVAQ 423
T + +M+ L++++
Sbjct: 546 TNNLLMLPLVMLVLLISK 563
Score = 27.5 bits (58), Expect = 5.7
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +2
Query: 422 KWIGDFFNEGIYDIYIQLAGVPLL 493
K + D FN G+YD + + G+P +
Sbjct: 563 KTVADCFNRGVYDQIVTMKGLPYM 586
>At2g25850.2 68415.m03104 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain; identical to cDNA GI:31747890
Length = 800
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
D S+ P A + +G+ +PL A P+ R +D+GLYE
Sbjct: 9 DDDSSQPVKASLKSYGITEPLSIAGPSAADVKRNLELEKFLVDEGLYE 56
>At2g25850.1 68415.m03103 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain; identical to cDNA GI:31747890
Length = 787
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
D S+ P A + +G+ +PL A P+ R +D+GLYE
Sbjct: 9 DDDSSQPVKASLKSYGITEPLSIAGPSAADVKRNLELEKFLVDEGLYE 56
>At5g45190.1 68418.m05547 cyclin family protein similar to cyclin T1
[Equus caballus] GI:5052355; contains Pfam profile
PF00134: Cyclin, N-terminal domain
Length = 579
Score = 28.3 bits (60), Expect = 3.2
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = -1
Query: 243 YSEQSSPRGAGQQVRYEQTAGNGQTERPRREQEVAYDEH 127
Y + P G +V E + G G +RP V+ DEH
Sbjct: 267 YEQNRVPASQGSEV--ESSVGGGSAQRPGSRNAVSTDEH 303
>At1g21695.1 68414.m02716 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 217
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -3
Query: 211 SAGSV*TNRWKRPDRTSTSRAGSSIRRT 128
S GS ++RW P + +SR GS ++RT
Sbjct: 169 SQGSPVSSRWSSPKSSFSSRRGSPLKRT 196
>At5g04620.2 68418.m00464 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 476
Score = 27.9 bits (59), Expect = 4.3
Identities = 16/72 (22%), Positives = 37/72 (51%)
Frame = +3
Query: 9 ILRXRRVQRASRDMVPNARSVGTIVPPRPDGLVQALVYTSVRRMLLPALDVDVRSGRFQR 188
+L R++ R+ R + + ++ IV R +G V+ + + +++V V FQ+
Sbjct: 16 VLESRQILRSLRPICMSRQNEEEIVKSRANGGDGYEVFDGLCQWDRTSVEVSVSIPTFQK 75
Query: 189 FVHTEPADRRRV 224
++H EP++ +
Sbjct: 76 WLHDEPSNGEEI 87
>At4g32850.6 68417.m04675 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 745
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
++ +LP +G+ KPL A P+ R +D+GLYE
Sbjct: 7 NLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYE 54
>At4g32850.5 68417.m04674 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 745
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
++ +LP +G+ KPL A P+ R +D+GLYE
Sbjct: 7 NLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYE 54
>At4g32850.4 68417.m04671 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 730
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
++ +LP +G+ KPL A P+ R +D+GLYE
Sbjct: 7 NLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYE 54
>At4g32850.3 68417.m04673 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 735
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
++ +LP +G+ KPL A P+ R +D+GLYE
Sbjct: 7 NLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYE 54
>At4g32850.2 68417.m04672 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 738
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
++ +LP +G+ KPL A P+ R +D+GLYE
Sbjct: 7 NLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYE 54
>At4g32850.1 68417.m04676 nucleotidyltransferase family protein
contains Pfam profiles: PF01909 nucleotidyltransferase
domain, PF04926 poly(A) polymerase predicted RNA
binding domain
Length = 741
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 245 DIASNLPHAAPVSRFGMNKPLETARPNVHVESRK*HTTNTSIDQGLYE 102
++ +LP +G+ KPL A P+ R +D+GLYE
Sbjct: 7 NLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYE 54
>At4g00540.1 68417.m00074 myb family transcription factor
Length = 437
Score = 26.6 bits (56), Expect = 9.9
Identities = 17/67 (25%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = -1
Query: 243 YSEQSSPRGAGQQVRYEQTAGNGQTERPRREQEVAYDEH*YRPGLVR-AHRVVEERSYRR 67
+S +SP + V+ +G+ ER + +DEH R G+V + R++ ++S +
Sbjct: 368 HSVSASPSSKYRFVKRNTCSGSKPLERRLDFDFLLWDEHGRRNGIVNFSVRILPQKSDLK 427
Query: 66 FGRLEPY 46
G + P+
Sbjct: 428 SGLVRPF 434
>At2g34560.2 68415.m04246 katanin, putative similar to katanin p60
subunit [Strongylocentrotus purpuratus] GI:3098603;
contains Pfam profile PF00004: ATPase AAA family
Length = 393
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +3
Query: 93 PDGLVQALVYTSVRRMLLPALDVDVRSGRFQRFVHTEPAD 212
P L A++ +R+L+P D + R G F+ + ++P D
Sbjct: 261 PWELDAAMLRRLEKRILVPLPDPEARRGMFEMLIPSQPGD 300
>At2g34560.1 68415.m04245 katanin, putative similar to katanin p60
subunit [Strongylocentrotus purpuratus] GI:3098603;
contains Pfam profile PF00004: ATPase AAA family
Length = 384
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +3
Query: 93 PDGLVQALVYTSVRRMLLPALDVDVRSGRFQRFVHTEPAD 212
P L A++ +R+L+P D + R G F+ + ++P D
Sbjct: 252 PWELDAAMLRRLEKRILVPLPDPEARRGMFEMLIPSQPGD 291
>At1g73590.1 68414.m08519 auxin efflux carrier protein, putative
(PIN1) identical to putative auxin efflux carrier
protein; AtPIN1 [Arabidopsis thaliana] GI:4151319;
contains Pfam profile PF03547: Auxin Efflux Carrier
Length = 622
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = +3
Query: 24 RVQRASRDMVPNARSVGTIVPPRPDGLVQALVYT 125
R ASR + + RS G PRP L A +Y+
Sbjct: 207 RRSNASRSDIYSRRSQGLSATPRPSNLTNAEIYS 240
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,430,402
Number of Sequences: 28952
Number of extensions: 244012
Number of successful extensions: 670
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 653
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 670
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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