Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31903
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 56 1e-08
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 54 4e-08
At2g31400.1 68415.m03837 pentatricopeptide (PPR) repeat-containi... 36 0.021
At5g20230.1 68418.m02408 plastocyanin-like domain-containing pro... 30 0.80
At2g43410.1 68415.m05395 RNA recognition motif (RRM)-containing ... 30 1.1
At1g64450.1 68414.m07306 proline-rich family protein contains pr... 30 1.1
At3g24550.1 68416.m03083 protein kinase family protein contains ... 29 1.9
At2g15530.2 68415.m01778 zinc finger (C3HC4-type RING finger) fa... 29 1.9
At2g15530.1 68415.m01777 zinc finger (C3HC4-type RING finger) fa... 29 1.9
At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein ... 29 2.5
At2g27630.1 68415.m03350 ubiquitin carboxyl-terminal hydrolase-r... 28 3.2
At1g11820.1 68414.m01358 glycosyl hydrolase family 17 protein si... 28 3.2
At4g08390.2 68417.m01386 L-ascorbate peroxidase, stromal (sAPX) ... 28 4.3
At4g08390.1 68417.m01385 L-ascorbate peroxidase, stromal (sAPX) ... 28 4.3
At1g65260.1 68414.m07398 PspA/IM30 family protein contains Pfam ... 28 4.3
At5g41680.2 68418.m05065 protein kinase family protein contains ... 27 5.7
At5g41680.1 68418.m05064 protein kinase family protein contains ... 27 5.7
At3g01180.1 68416.m00023 glycogen synthase, putative similar to ... 27 5.7
At1g09460.1 68414.m01058 glucan endo-1,3-beta-glucosidase-relate... 27 7.5
At5g24990.1 68418.m02961 expressed protein 27 9.9
At5g10880.1 68418.m01263 tRNA synthetase-related / tRNA ligase-r... 27 9.9
At3g43540.2 68416.m04623 expressed protein hypothetical protein ... 27 9.9
At3g43540.1 68416.m04622 expressed protein hypothetical protein ... 27 9.9
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1025
Score = 56.0 bits (129), Expect = 1e-08
Identities = 29/67 (43%), Positives = 38/67 (56%)
Frame = +2
Query: 149 QSERFASWLLNKPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETMYNAWLADPNSVHAS 328
Q+ F S +L +A V + S + FL+G+SS Y+E + AW ADPNSV S
Sbjct: 37 QTRCFHSTILKSKAESAAPV-PRPVPLSKLTDSFLDGTSSVYLEELQRAWEADPNSVDES 95
Query: 329 WDAFFRN 349
WD FFRN
Sbjct: 96 WDNFFRN 102
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1017
Score = 54.4 bits (125), Expect = 4e-08
Identities = 23/40 (57%), Positives = 28/40 (70%)
Frame = +2
Query: 230 STAAEPFLNGSSSAYVETMYNAWLADPNSVHASWDAFFRN 349
S + FL+G+SS Y+E + AW ADPNSV SWD FFRN
Sbjct: 60 SKLTDSFLDGTSSVYLEELQRAWEADPNSVDESWDNFFRN 99
>At2g31400.1 68415.m03837 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 918
Score = 35.5 bits (78), Expect = 0.021
Identities = 25/70 (35%), Positives = 33/70 (47%)
Frame = +3
Query: 183 NHRLPRFQ*MPIG*RAPPRRNPSSMAPVQLTSRPCTMHGSPTQTPYTRLGMHFSAMRQTE 362
NHR P G APP + +++AP QL+ P + SP QTP + L FS R T
Sbjct: 88 NHRPYGASSSPRG-SAPPPSSVATVAPAQLSQPP---NFSPLQTPKSDLSSDFSGRRSTR 143
Query: 363 LSQAPHTRRR 392
H R+
Sbjct: 144 FVSKMHFGRQ 153
>At5g20230.1 68418.m02408 plastocyanin-like domain-containing
protein
Length = 196
Score = 30.3 bits (65), Expect = 0.80
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 2/46 (4%)
Frame = +2
Query: 350 ATNGAQPGAAYTPPPNLAPYNKNEVPLTSLVPSSGG--MPSISAGS 481
AT GA PGA TP P P P P++GG PS S+G+
Sbjct: 128 ATGGATPGAGATPAPGSTPSTGGTTP-----PTAGGTTTPSGSSGT 168
>At2g43410.1 68415.m05395 RNA recognition motif (RRM)-containing
protein contains InterPro entry IPR000504: RNA-binding
region RNP-1 (RNA recognition motif) (RRM)
Length = 1056
Score = 29.9 bits (64), Expect = 1.1
Identities = 16/53 (30%), Positives = 21/53 (39%)
Frame = +2
Query: 278 ETMYNAWLADPNSVHASWDAFFRNATNGAQPGAAYTPPPNLAPYNKNEVPLTS 436
E AW P +VH + + F+ N P PPP+ P N TS
Sbjct: 841 EAPSQAWKRGPQTVHDASNQSFQQYGNQYTPAGQLPPPPSRYPPASNNPNYTS 893
>At1g64450.1 68414.m07306 proline-rich family protein contains
proline rich extensins, INTERPRO:IPR0002965
Length = 342
Score = 29.9 bits (64), Expect = 1.1
Identities = 31/111 (27%), Positives = 49/111 (44%), Gaps = 2/111 (1%)
Frame = +2
Query: 182 KPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETMYNAWLADPN-SVHASWDAFFRNATN 358
KP+ +SVNA +L S + N S S YV + +PN +V + +D+ + +
Sbjct: 42 KPKDPKISVNAVQLPSFAVSNNTANFSFSQYVA------VRNPNRAVFSHYDSSIQLLYS 95
Query: 359 GAQPGAAYTPPPNLAPYNKNEVPLTSLVPSSG-GMPSISAGSPINEKIIDD 508
G Q G + P + + T V S PS SA S ++ +I D
Sbjct: 96 GNQVGFMFIPAGKIDSGRIQYMAATFTVHSFPISPPSSSAISTVSAAVIPD 146
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 29.1 bits (62), Expect = 1.9
Identities = 19/49 (38%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
Frame = +2
Query: 347 NATNGAQPGAAYTPPPNLAPYNKNEVP---LTSLVPSSGGMPSISAGSP 484
N+T P AA +PPP P + P TS PSS PS+ SP
Sbjct: 21 NSTTTTPPPAASSPPPTTTPSSPPPSPSTNSTSPPPSSPLPPSLPPPSP 69
>At2g15530.2 68415.m01778 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 704
Score = 29.1 bits (62), Expect = 1.9
Identities = 38/149 (25%), Positives = 61/149 (40%), Gaps = 15/149 (10%)
Frame = +2
Query: 86 GS*QITIMHRARIVFNISKSGQSERFASWLLNKPQTAAVSVNANRL------KSSTAAEP 247
G Q I+H + NI + F+S N + + + A RL S +P
Sbjct: 351 GENQTNIVHLPALTRNIHQYAWDASFSSRASNP---SGIGMPAERLGPQWETPRSNQEQP 407
Query: 248 FLNGSSSAYVETMYNAWLADPNSVHASWDAFF------RNATNGAQPGAAYTPPPNLAPY 409
L ++ + +++ W S +S D+ F +A + QP + PP N P+
Sbjct: 408 -LFAPATDMRQPVHDLWNFARGSPGSSVDSLFVPRAGPSSAIHTPQPNPTWIPPQNAPPH 466
Query: 410 N---KNEVPLTSLVPSSGGMPSISAGSPI 487
N +E+ SL PS PS S G P+
Sbjct: 467 NPSRTSELSPWSLFPSIES-PSASHGGPL 494
>At2g15530.1 68415.m01777 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 704
Score = 29.1 bits (62), Expect = 1.9
Identities = 38/149 (25%), Positives = 61/149 (40%), Gaps = 15/149 (10%)
Frame = +2
Query: 86 GS*QITIMHRARIVFNISKSGQSERFASWLLNKPQTAAVSVNANRL------KSSTAAEP 247
G Q I+H + NI + F+S N + + + A RL S +P
Sbjct: 351 GENQTNIVHLPALTRNIHQYAWDASFSSRASNP---SGIGMPAERLGPQWETPRSNQEQP 407
Query: 248 FLNGSSSAYVETMYNAWLADPNSVHASWDAFF------RNATNGAQPGAAYTPPPNLAPY 409
L ++ + +++ W S +S D+ F +A + QP + PP N P+
Sbjct: 408 -LFAPATDMRQPVHDLWNFARGSPGSSVDSLFVPRAGPSSAIHTPQPNPTWIPPQNAPPH 466
Query: 410 N---KNEVPLTSLVPSSGGMPSISAGSPI 487
N +E+ SL PS PS S G P+
Sbjct: 467 NPSRTSELSPWSLFPSIES-PSASHGGPL 494
>At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein
contains Pfam profile: PF01363 FYVE zinc finger
Length = 601
Score = 28.7 bits (61), Expect = 2.5
Identities = 29/102 (28%), Positives = 40/102 (39%), Gaps = 7/102 (6%)
Frame = +2
Query: 185 PQTAAVSVNANRLKSSTAAEP---FLNGSSSAYVETMYNAWLADPNSVHASWDAFFRNAT 355
P A S+N N + P S+Y A P S H + + ++ T
Sbjct: 96 PSPPATSLNPNSYSTFNQPPPPPTIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQT 155
Query: 356 NGAQ----PGAAYTPPPNLAPYNKNEVPLTSLVPSSGGMPSI 469
+G P +A P PN APY+ + L S P S G SI
Sbjct: 156 SGYSSAPPPSSAPAPNPNPAPYSSS---LYSAPPYSSGGSSI 194
>At2g27630.1 68415.m03350 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF04780:
Protein of unknown function (DUF629), PF04781: Protein
of unknown function (DUF627)
Length = 1122
Score = 28.3 bits (60), Expect = 3.2
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 151 LARFRDVKHYPCSMHDCYLL*P 86
L FRD+K PCS+ DC + P
Sbjct: 353 LVMFRDLKILPCSVRDCVMQYP 374
>At1g11820.1 68414.m01358 glycosyl hydrolase family 17 protein
similar to elicitor inducible chitinase Nt-SubE76
GI:11071974 from [Nicotiana tabacum]
Length = 511
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 260 SSSAYVETMYNAWLADPNSVHASWDAFFRNAT 355
+SS Y+ ++N L P ASW F+ N+T
Sbjct: 327 TSSVYIYELFNEDLRAPPVSEASWGLFYGNST 358
>At4g08390.2 68417.m01386 L-ascorbate peroxidase, stromal (sAPX)
identical to stromal ascorbate peroxidase [Arabidopsis
thaliana] gi|1419388|emb|CAA67425
Length = 372
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 173 LLNKPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETM 286
LL+ P T + ++ L+S+TAA L SSS+ T+
Sbjct: 13 LLSPPPTTTTTTMSSSLRSTTAASLLLRSSSSSSRSTL 50
>At4g08390.1 68417.m01385 L-ascorbate peroxidase, stromal (sAPX)
identical to stromal ascorbate peroxidase [Arabidopsis
thaliana] gi|1419388|emb|CAA67425
Length = 372
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 173 LLNKPQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETM 286
LL+ P T + ++ L+S+TAA L SSS+ T+
Sbjct: 13 LLSPPPTTTTTTMSSSLRSTTAASLLLRSSSSSSRSTL 50
>At1g65260.1 68414.m07398 PspA/IM30 family protein contains Pfam
PF04012: PspA/IM30 family profile; similar to
Membrane-associated 30 kDa protein, chloroplast
precursor (M30) (Swiss-Prot:Q03943) [Pisum sativum];
similar to phage shock protein A (GI:28806161) [Vibrio
parahaemolyticus]; similar to Phage shock protein A.
(Swiss-Prot:P23853) [Shigella flexneri]
Length = 330
Score = 27.9 bits (59), Expect = 4.3
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +3
Query: 231 PPRRNPSSMAPVQLTSRPCTMHGSPTQTPYTRLGMHFSAMRQTELSQAPHTRRR 392
PP R +S +P ++RPC++ P +T + G A+R L A R R
Sbjct: 13 PPLRPTASSSPSTSSNRPCSLRILPLRTSF--FGNSSGALRVNVLRLACDNRLR 64
>At5g41680.2 68418.m05065 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
receptor-like protein kinase (GI:4008006) [Arabidopsis
thaliana]; similar to receptor-like kinase RHG1
(GI:21239380) (GI:21239382) [Glycine max]
Length = 333
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 383 CMRRLAELRLSHCGKMHPKTRVRSLGRRAMHCTWSRRKLNWS 258
C + +L L+H K P+T +RS G A T +R+ +S
Sbjct: 194 CYGCICDLGLTHITKSLPQTTLRSSGYHAPEITDTRKSTQFS 235
>At5g41680.1 68418.m05064 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
receptor-like protein kinase (GI:4008006) [Arabidopsis
thaliana]; similar to receptor-like kinase RHG1
(GI:21239380) (GI:21239382) [Glycine max]
Length = 359
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 383 CMRRLAELRLSHCGKMHPKTRVRSLGRRAMHCTWSRRKLNWS 258
C + +L L+H K P+T +RS G A T +R+ +S
Sbjct: 220 CYGCICDLGLTHITKSLPQTTLRSSGYHAPEITDTRKSTQFS 261
>At3g01180.1 68416.m00023 glycogen synthase, putative similar to
glycogen synthase Q43847 from [Solanum tuberosum]
Length = 792
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = +3
Query: 231 PPRRNPSSMAPVQLTSRPCTMHGSPTQTPYTRLGMHFSAMRQTELSQAPHT 383
PP + S M + TS P T G P++ +R G +S + L++AP T
Sbjct: 215 PPYKPSSVMTSPEKTSDPVTSPGKPSK---SRAGAFWSDPLPSYLTKAPQT 262
>At1g09460.1 68414.m01058 glucan endo-1,3-beta-glucosidase-related
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]
Length = 330
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +2
Query: 347 NATNGAQPGAAYTPPPNLAPYNKNEVPLTSLVPSSGGMPSISAGSP 484
++T+ TP P+ N+ V T ++P+ GG+ I G+P
Sbjct: 222 SSTSTPMTAGTTTPTPSTQTVNQPPVTSTPIIPTGGGI--IGVGTP 265
>At5g24990.1 68418.m02961 expressed protein
Length = 294
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/46 (32%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Frame = +2
Query: 251 LNGSSSAYV--ETMYNAWLADPNSVHASWDAFFRNATNGAQPGAAY 382
+NG S +V ET N W + P +V + + AT PG Y
Sbjct: 33 INGGSFCHVDLETGTNGWASPPGNVFSLRSHNYFTATKQKSPGGDY 78
>At5g10880.1 68418.m01263 tRNA synthetase-related / tRNA
ligase-related similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profile PF03129:
Anticodon binding domain
Length = 309
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 145 RFRDVKHYPCSMHDCYLL*PPGVSD 71
RF ++ Y S+ CY+L P G+SD
Sbjct: 26 RFGELVEYYESVKGCYILKPSGISD 50
>At3g43540.2 68416.m04623 expressed protein hypothetical protein
slr1699 - Synechocystis sp. (strain PCC 6803),
PIR:S75306
Length = 301
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 386 PPPNLAPYNKNEVPLTSLVPSSGGMPSISAGSPINEKI 499
P NL P + ++PL S+ S+G + + GS EKI
Sbjct: 59 PNSNLKPGDLADLPLFSVGHSNGALLQVLTGSYFAEKI 96
>At3g43540.1 68416.m04622 expressed protein hypothetical protein
slr1699 - Synechocystis sp. (strain PCC 6803),
PIR:S75306
Length = 373
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 386 PPPNLAPYNKNEVPLTSLVPSSGGMPSISAGSPINEKI 499
P NL P + ++PL S+ S+G + + GS EKI
Sbjct: 131 PNSNLKPGDLADLPLFSVGHSNGALLQVLTGSYFAEKI 168
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,690,520
Number of Sequences: 28952
Number of extensions: 247440
Number of successful extensions: 849
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 794
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 846
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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