Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31897
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 103 9e-23
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 100 1e-21
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 99 2e-21
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 96 1e-20
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 94 6e-20
At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 33 0.15
At4g22260.1 68417.m03220 alternative oxidase, putative / immutan... 29 1.4
At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 29 1.9
At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 3.2
At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 28 4.3
At4g37390.1 68417.m05294 auxin-responsive GH3 family protein sim... 27 5.7
At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma m... 27 7.5
At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma m... 27 9.9
At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma m... 27 9.9
At1g23550.1 68414.m02962 expressed protein 27 9.9
At1g12940.1 68414.m01503 high-affinity nitrate transporter, puta... 27 9.9
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 103 bits (246), Expect = 9e-23
Identities = 56/133 (42%), Positives = 77/133 (57%), Gaps = 8/133 (6%)
Frame = +3
Query: 39 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 197
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ F+
Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60
Query: 198 SLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 374
L G+ +AG +Y+ + G + +IR K G G+ KT Q++V LYEEP+ P Q
Sbjct: 61 GHLAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNM 120
Query: 375 VVEKLGEYLITCG 413
VVE+LG+YLI G
Sbjct: 121 VVERLGDYLIEQG 133
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 99.5 bits (237), Expect = 1e-21
Identities = 55/130 (42%), Positives = 73/130 (56%), Gaps = 5/130 (3%)
Frame = +3
Query: 39 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 206
MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I F L
Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97
Query: 207 TSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 383
G+ + G +Y+ + G + +IR K G GV KT A+V +Y+EP+ P Q VVE
Sbjct: 98 APTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVE 157
Query: 384 KLGEYLITCG 413
LGEYLI G
Sbjct: 158 NLGEYLIESG 167
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 98.7 bits (235), Expect = 2e-21
Identities = 54/133 (40%), Positives = 75/133 (56%), Gaps = 8/133 (6%)
Frame = +3
Query: 39 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 197
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E + I+ F+
Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60
Query: 198 SLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 374
L G+ +AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q
Sbjct: 61 GHLAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNM 120
Query: 375 VVEKLGEYLITCG 413
VVE+LG+YL+ G
Sbjct: 121 VVERLGDYLLEQG 133
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 96.3 bits (229), Expect = 1e-20
Identities = 55/130 (42%), Positives = 73/130 (56%), Gaps = 5/130 (3%)
Frame = +3
Query: 39 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 206
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I FE L
Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60
Query: 207 TSGGVTIAGTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 383
G+ + G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q VVE
Sbjct: 61 APTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVE 120
Query: 384 KLGEYLITCG 413
+LG+YLI G
Sbjct: 121 RLGDYLIESG 130
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 93.9 bits (223), Expect = 6e-20
Identities = 53/127 (41%), Positives = 70/127 (55%), Gaps = 5/127 (3%)
Frame = +3
Query: 39 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 206
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+ I FE L
Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60
Query: 207 TSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 383
G+ + G +Y+ + G +IR K G GV KT QA+V Y+EP+ Q VVE
Sbjct: 61 APTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVE 120
Query: 384 KLGEYLI 404
+LG+YLI
Sbjct: 121 RLGDYLI 127
>At1g62420.1 68414.m07042 expressed protein contains Pfam profile
PF04720: Protein of unknown function (DUF506)
Length = 465
Score = 32.7 bits (71), Expect = 0.15
Identities = 16/63 (25%), Positives = 32/63 (50%)
Frame = +2
Query: 197 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 376
I +E RR +H+P W R + + W G ++ T++ ++ + T+ T+A + C
Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255
Query: 377 RGE 385
+ E
Sbjct: 256 KEE 258
>At4g22260.1 68417.m03220 alternative oxidase, putative / immutans
protein (IM) identical to IMMUTANS from Arabidopsis
thaliana [gi:4138855]; contains Pfam profile PF01786
alternative oxidase
Length = 351
Score = 29.5 bits (63), Expect = 1.4
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = -1
Query: 516 LITL*NIQNAVTYFPVEKILSYSTLRSRRASTSNNHR 406
L+TL + AV+Y ++L + L SRR NNHR
Sbjct: 18 LVTLRRSRAAVSYSSSHRLLHHLPLSSRRLLLRNNHR 54
>At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar
to sodium/calcium exchanger protein [Mus musculus]
gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 559
Score = 29.1 bits (62), Expect = 1.9
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -3
Query: 271 MIWSVPLR*MYRVPAIVTPPLVSSDSFSKPATI 173
+IW++ L +P I+T P+VS D +SKP +
Sbjct: 307 VIWAITLP--LNLPRILTIPVVSEDKWSKPLAV 337
>At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA7)
identical to SP|O64806 Potential calcium-transporting
ATPase 7, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong
similarity to SP|O81108 Calcium-transporting ATPase 2,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
2) {Arabidopsis thaliana}
Length = 1015
Score = 28.3 bits (60), Expect = 3.2
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 132 KSEGFEISKDEVAKIVAGFENESLLTSGGV 221
K+ GF+I DE+ IV G + + L GGV
Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119
>At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma
membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical
to SP|O22218 Calcium-transporting ATPase 4, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4)
{Arabidopsis thaliana}
Length = 1030
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 132 KSEGFEISKDEVAKIVAGFENESLLTSGGV 221
K GF I DE+A +V + +SL GGV
Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGV 118
>At4g37390.1 68417.m05294 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 603
Score = 27.5 bits (58), Expect = 5.7
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = +3
Query: 15 YF*LNLIKMSWQDYVDKQLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVAGFEN 194
YF +NL M V +M + + HDG+ A++ E S E+A + G E
Sbjct: 344 YFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEY 403
Query: 195 ESLLTS 212
E ++T+
Sbjct: 404 ELVITT 409
>At4g37640.1 68417.m05324 calcium-transporting ATPase 2, plasma
membrane-type / Ca(2+)-ATPase isoform 2 (ACA2) identical
to SP|O81108 Calcium-transporting ATPase 2, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2)
{Arabidopsis thaliana}
Length = 1014
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 132 KSEGFEISKDEVAKIVAGFENESLLTSGGV 221
K+ GFEI DE+ IV + + L GGV
Sbjct: 90 KAAGFEICADELGSIVESHDVKKLKFHGGV 119
>At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 946
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 132 KSEGFEISKDEVAKIVAGFENESLLTSGG 218
+ GFEI DE+ IV G + + L GG
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGG 120
>At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 1020
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 132 KSEGFEISKDEVAKIVAGFENESLLTSGG 218
+ GFEI DE+ IV G + + L GG
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGG 120
>At1g23550.1 68414.m02962 expressed protein
Length = 323
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/47 (29%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = +3
Query: 66 QLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVA-GFENESL 203
+ +A R T A H G+ K + S+DE+ +I++ GF N +
Sbjct: 92 KFLAFRIFTDAVARKHGGDANVKYGWYAGSRDEIQRIISYGFSNRDV 138
>At1g12940.1 68414.m01503 high-affinity nitrate transporter,
putative similar to trans-membrane nitrate transporter
protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058,
high-affinity nitrate transporter ACH1 [Arabidopsis
thaliana] GI:3608362
Length = 502
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/28 (39%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = +3
Query: 114 DGNVWAKSEGFEISKDEVAKIVA-GFEN 194
DG+ WA + E KD+V K+++ G +N
Sbjct: 233 DGDYWAMHKSGEREKDDVGKVISNGIKN 260
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,503,138
Number of Sequences: 28952
Number of extensions: 235437
Number of successful extensions: 553
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 532
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 543
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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