Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31892
(506 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 236 2e-63
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 236 2e-63
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 236 2e-63
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 74 1e-14
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 60 2e-10
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 54 2e-08
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 34 0.011
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 1.6
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 2.1
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 2.8
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 3.7
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 4.9
SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce... 25 6.5
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 6.5
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 25 6.5
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 25 8.6
SPAPB24D3.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 8.6
SPAPB1A10.11c |||glutamyl-tRNA synthetase, mitochondrial|Schizos... 25 8.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 236 bits (577), Expect = 2e-63
Identities = 105/136 (77%), Positives = 119/136 (87%)
Frame = +2
Query: 98 DNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGG 277
DNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGG
Sbjct: 197 DNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGG 256
Query: 278 IGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVK 457
IGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK
Sbjct: 257 IGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVK 316
Query: 458 ELRRGYVAGDSKNNPP 505
++RRG V GDSKN+PP
Sbjct: 317 DIRRGNVCGDSKNDPP 332
Score = 42.7 bits (96), Expect = 3e-05
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = +1
Query: 16 KEVSSYIKKIGYNPAAVAFVPISGWHG 96
KE S++IKK+G+NP V FVP+SG+ G
Sbjct: 170 KETSNFIKKVGFNPKTVPFVPVSGFQG 196
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 236 bits (577), Expect = 2e-63
Identities = 105/136 (77%), Positives = 119/136 (87%)
Frame = +2
Query: 98 DNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGG 277
DNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGG
Sbjct: 197 DNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGG 256
Query: 278 IGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVK 457
IGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK
Sbjct: 257 IGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVK 316
Query: 458 ELRRGYVAGDSKNNPP 505
++RRG V GDSKN+PP
Sbjct: 317 DIRRGNVCGDSKNDPP 332
Score = 42.7 bits (96), Expect = 3e-05
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = +1
Query: 16 KEVSSYIKKIGYNPAAVAFVPISGWHG 96
KE S++IKK+G+NP V FVP+SG+ G
Sbjct: 170 KETSNFIKKVGFNPKTVPFVPVSGFQG 196
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 236 bits (577), Expect = 2e-63
Identities = 105/136 (77%), Positives = 119/136 (87%)
Frame = +2
Query: 98 DNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGG 277
DNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGG
Sbjct: 197 DNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGG 256
Query: 278 IGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVK 457
IGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK
Sbjct: 257 IGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVK 316
Query: 458 ELRRGYVAGDSKNNPP 505
++RRG V GDSKN+PP
Sbjct: 317 DIRRGNVCGDSKNDPP 332
Score = 42.7 bits (96), Expect = 3e-05
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = +1
Query: 16 KEVSSYIKKIGYNPAAVAFVPISGWHG 96
KE S++IKK+G+NP V FVP+SG+ G
Sbjct: 170 KETSNFIKKVGFNPKTVPFVPVSGFQG 196
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 73.7 bits (173), Expect = 1e-14
Identities = 39/101 (38%), Positives = 61/101 (60%), Gaps = 3/101 (2%)
Frame = +2
Query: 185 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPAN 355
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V ++
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 356 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 478
+ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 294 LKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 59.7 bits (138), Expect = 2e-10
Identities = 36/108 (33%), Positives = 57/108 (52%), Gaps = 1/108 (0%)
Frame = +2
Query: 176 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 355
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ +
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQ 442
Query: 356 ITTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKN 496
VK+V + + AV GD V + ++ V +LR G + + +N
Sbjct: 443 EDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYEN 490
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 53.6 bits (123), Expect = 2e-08
Identities = 32/108 (29%), Positives = 56/108 (51%), Gaps = 1/108 (0%)
Frame = +2
Query: 176 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 355
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N
Sbjct: 448 GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPIN 505
Query: 356 ITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKN 496
T EV ++ + E + ++ GD V V+ +++ GYV +KN
Sbjct: 506 QTLEVTAIYDEADEEISSSICGDQVRLRVRG-DDSDVQTGYVLTSTKN 552
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 34.3 bits (75), Expect = 0.011
Identities = 31/113 (27%), Positives = 49/113 (43%), Gaps = 5/113 (4%)
Frame = +2
Query: 125 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 304
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 305 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEA--VPGDNVGF---NVKNV 448
G +K G V+ EV+ V + + + E + VG+ N+KN+
Sbjct: 266 MEGFIKKGGKVMSVNTGRKYEVQQVGIMYPDMTEVSRLRAGQVGYIIWNMKNI 318
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 1.6
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -3
Query: 369 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 253
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 463 QFLDGHVLYVETYIVSRYSFLESFV 389
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 2.8
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -3
Query: 327 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 163
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 162 SLRSTCHPLNQG 127
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 3.7
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -2
Query: 487 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 371
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 4.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 318 NTPVSTLPTGTVPIPPIL 265
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1112
Score = 25.0 bits (52), Expect = 6.5
Identities = 15/55 (27%), Positives = 24/55 (43%)
Frame = +2
Query: 86 DGTXDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLP 250
DGT N L S+ + + + K G E++DA+LP + L +P
Sbjct: 610 DGTVTNPLNVSSDKA-MSVYLLSSENAKDTGDIKSESIDAVLPTLETSSPSLSIP 663
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 25.0 bits (52), Expect = 6.5
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = -3
Query: 405 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPT 292
SW SW +ST L +V + G T ST T
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRT 134
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 25.0 bits (52), Expect = 6.5
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = +3
Query: 72 RAHFWMARXTTCWSLQPKCLGSRDGRWSVKKAKLTENASLKLSMPSCHLPAPL 230
R H + SL+ K +GS+D S + L+E+ SLK + H PL
Sbjct: 515 RTHLASLLSSLLGSLRDKNIGSKDTTVSQIASILSEDLSLKREIIQAHGILPL 567
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 24.6 bits (51), Expect = 8.6
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +1
Query: 19 EVSSYIKKIGYNPAAVAFVPISGWHGRQH 105
EVS I +P AFV + G HG H
Sbjct: 1571 EVSGDYNPIHVSPTFAAFVELPGTHGITH 1599
>SPAPB24D3.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 300
Score = 24.6 bits (51), Expect = 8.6
Identities = 10/36 (27%), Positives = 17/36 (47%)
Frame = -3
Query: 387 CISTDLTSVVMLAGAKTTMVPGFNTPVSTLPTGTVP 280
C++T + L T NT +++ P GT+P
Sbjct: 247 CVATFTSGYSPLVEPAFTFASALNTTLNSFPNGTLP 282
>SPAPB1A10.11c |||glutamyl-tRNA synthetase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 526
Score = 24.6 bits (51), Expect = 8.6
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +1
Query: 4 RKSXKEVSSYIKKIGYNPAAVA-FVPISGWHGRQ 102
R++ VSS +++ G+ P A+ F+ + GW RQ
Sbjct: 282 RQNDAHVSSLLQE-GFLPEAILNFIALMGWSSRQ 314
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,300,648
Number of Sequences: 5004
Number of extensions: 49075
Number of successful extensions: 198
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -