BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31883 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 320 1e-88 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 180 1e-46 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 167 9e-43 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 99 3e-22 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 94 1e-20 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 86 3e-18 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 49 2e-11 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 1e-08 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.2 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 26 3.8 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.1 SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosacchar... 25 6.7 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 8.9 SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.9 SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 320 bits (785), Expect = 1e-88 Identities = 150/172 (87%), Positives = 161/172 (93%) Frame = +1 Query: 1 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLC 180 GV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLC Sbjct: 158 GVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLC 217 Query: 181 YVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHET 360 YVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ GIHE Sbjct: 218 YVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEA 277 Query: 361 VYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKI 516 YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI Sbjct: 278 TYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKI 329 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 180 bits (438), Expect = 1e-46 Identities = 81/163 (49%), Positives = 114/163 (69%) Frame = +1 Query: 1 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLC 180 GV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLC Sbjct: 158 GVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLC 217 Query: 181 YVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHET 360 YV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E Sbjct: 218 YVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEF 277 Query: 361 VYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL 489 ++++I DVDIRK LY V+SGG++MY G+ R++KEI L Sbjct: 278 IFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQL 320 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 167 bits (406), Expect = 9e-43 Identities = 74/172 (43%), Positives = 114/172 (66%) Frame = +1 Query: 1 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLC 180 G++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK C Sbjct: 163 GLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCC 222 Query: 181 YVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHET 360 YVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ G+H Sbjct: 223 YVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQ 282 Query: 361 VYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKI 516 ++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ ++KI Sbjct: 283 LFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKI 334 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 99.1 bits (236), Expect = 3e-22 Identities = 60/190 (31%), Positives = 97/190 (51%), Gaps = 20/190 (10%) Frame = +1 Query: 7 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 186 +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV Sbjct: 168 THIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNMME--ETYIVNEIKESVCYV 225 Query: 187 ALDFEQEMATAAAS--TSLEKSYELPD------------------GQVITIGNERFRCPE 306 + +F+++M + LE Y LPD QV+ + NERF PE Sbjct: 226 SQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNERFMIPE 285 Query: 307 ALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITA 486 LF PS + + GI E V S+ +I+ L N V GG +PG R+ E+ + Sbjct: 286 LLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRS 345 Query: 487 LAPSTIKIKI 516 LAP+ ++K+ Sbjct: 346 LAPANWEVKV 355 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 94.3 bits (224), Expect = 1e-20 Identities = 59/175 (33%), Positives = 90/175 (51%), Gaps = 7/175 (4%) Frame = +1 Query: 1 GVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEK 174 GV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER IKE+ Sbjct: 189 GVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEE 243 Query: 175 LCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFLGMES 342 CYV D +E + L+ + E G TI G ERF PE F P + Sbjct: 244 CCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDF 303 Query: 343 CG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI 504 + E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 304 LTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERI 358 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 85.8 bits (203), Expect = 3e-18 Identities = 48/132 (36%), Positives = 70/132 (53%), Gaps = 9/132 (6%) Frame = +1 Query: 124 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 303 S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299 Query: 304 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 456 E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+ Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359 Query: 457 ADRMQKEITALA 492 ++R+Q E+ LA Sbjct: 360 SERLQAEVQRLA 371 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 48.8 bits (111), Expect(2) = 2e-11 Identities = 24/56 (42%), Positives = 35/56 (62%), Gaps = 1/56 (1%) Frame = +1 Query: 346 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKI 510 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P S +KI Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGSRLKI 381 Score = 34.3 bits (75), Expect(2) = 2e-11 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +1 Query: 217 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 321 ++ +T E +E PDG + G ER++ PE LF P Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 54.4 bits (125), Expect = 1e-08 Identities = 22/77 (28%), Positives = 47/77 (61%) Frame = +1 Query: 286 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 465 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 466 MQKEITALAPSTIKIKI 516 +++E+T++ P I + Sbjct: 654 IKRELTSIMPVGSSINV 670 Score = 31.5 bits (68), Expect = 0.077 Identities = 18/79 (22%), Positives = 33/79 (41%) Frame = +1 Query: 7 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 186 SH +P+ G + R+ G + YL+K+ + SF + C+V Sbjct: 177 SHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHV 236 Query: 187 ALDFEQEMATAAASTSLEK 243 + D+ ++A A LE+ Sbjct: 237 SSDYTHDIAHALDRDILER 255 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 2.2 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 23 STKVTLCPTPSSVWTW 70 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.8 bits (54), Expect = 3.8 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = +1 Query: 400 KDLYANTVMSGGTTMYPGIADRMQKEI 480 K+LY++ ++ GG +PG A +++ I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERI 553 Score = 25.4 bits (53), Expect = 5.1 Identities = 9/36 (25%), Positives = 21/36 (58%) Frame = +1 Query: 22 IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 129 + EG +P++ ++++ G D+T MK+L + + Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 5.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +3 Query: 351 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 479 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 327 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +3 Query: 24 LRRLRSAPRHPPSGLGWSRLDRLPHED 104 L++ + +H G WS LDR H+D Sbjct: 254 LKKSKRVKKHDYGGEDWSLLDRKFHDD 280 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = +1 Query: 178 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 303 CYV+ +E + + SL SY +PD + I + R+ P Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81 >SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 24.6 bits (51), Expect = 8.9 Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%) Frame = +2 Query: 8 PTPYPSTKVTLC-PTP 52 P P P TKV +C PTP Sbjct: 256 PAPSPETKVLICGPTP 271 >SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 24.6 bits (51), Expect = 8.9 Identities = 16/36 (44%), Positives = 19/36 (52%) Frame = +1 Query: 163 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 270 IKEKL VA EQE A +SL+ S + G V Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,852,458 Number of Sequences: 5004 Number of extensions: 35042 Number of successful extensions: 142 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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