BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31880 (487 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 300 6e-83 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 166 1e-42 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 154 8e-39 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 119 2e-28 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 93 2e-20 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 90 1e-19 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 81 7e-17 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 42 4e-05 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 36 0.004 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.092 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.16 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.6 SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 6.1 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 6.1 SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.0 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 8.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 300 bits (737), Expect = 6e-83 Identities = 134/149 (89%), Positives = 144/149 (96%) Frame = +2 Query: 41 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 220 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 221 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 400 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 401 IMFETFNSPAMYVAIQAVLSLYAXGRTTG 487 I+FETFN+PA YVAIQAVLSLYA GRTTG Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTG 150 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 166 bits (404), Expect = 1e-42 Identities = 77/148 (52%), Positives = 103/148 (69%), Gaps = 1/148 (0%) Frame = +2 Query: 47 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 226 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 227 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 403 +L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 404 MFETFNSPAMYVAIQAVLSLYAXGRTTG 487 +ET N PA+ ++Q VL+LYA RTTG Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTG 155 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 154 bits (373), Expect = 8e-39 Identities = 73/147 (49%), Positives = 104/147 (70%), Gaps = 2/147 (1%) Frame = +2 Query: 53 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 226 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 227 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 406 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 407 FETFNSPAMYVAIQAVLSLYAXGRTTG 487 FE + +YVAIQAVLSLYA G ++G Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSG 150 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 119 bits (287), Expect = 2e-28 Identities = 66/161 (40%), Positives = 90/161 (55%), Gaps = 24/161 (14%) Frame = +2 Query: 59 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 187 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 188 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 346 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 347 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYA 469 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L A Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAA 167 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.5 bits (222), Expect = 2e-20 Identities = 51/147 (34%), Positives = 79/147 (53%) Frame = +2 Query: 44 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 223 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 224 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 403 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 404 MFETFNSPAMYVAIQAVLSLYAXGRTT 484 MFE+ PA Y+A Q + +A G+ T Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGT 147 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 90.2 bits (214), Expect = 1e-19 Identities = 48/147 (32%), Positives = 85/147 (57%), Gaps = 1/147 (0%) Frame = +2 Query: 29 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 208 F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72 Query: 209 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 385 + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +TE NP R Sbjct: 73 TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131 Query: 386 EKMTQIMFETFNSPAMYVAIQAVLSLY 466 MT+++FE +N+P++ I + S Y Sbjct: 132 STMTELLFELYNAPSVAYGIDGLFSFY 158 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 81.4 bits (192), Expect = 7e-17 Identities = 44/149 (29%), Positives = 81/149 (54%), Gaps = 2/149 (1%) Frame = +2 Query: 44 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 223 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 224 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 397 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 398 QIMFETFNSPAMYVAIQAVLSLYAXGRTT 484 + FE + PA Y+ QAV +A ++T Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKST 150 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 42.3 bits (95), Expect = 4e-05 Identities = 24/81 (29%), Positives = 38/81 (46%), Gaps = 4/81 (4%) Frame = +2 Query: 245 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 412 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 413 TFNSPAMYVAIQAVLSLYAXG 475 P +A + ++ LYA G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIG 192 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 35.5 bits (78), Expect = 0.004 Identities = 35/146 (23%), Positives = 60/146 (41%), Gaps = 4/146 (2%) Frame = +2 Query: 59 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 238 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 239 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 406 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 407 FETFNSPAMYVAIQAVLSLYAXGRTT 484 FE F A A L + G T Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFT 143 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.092 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 104 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 247 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.16 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +2 Query: 305 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 448 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 2.6 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 347 LLTEAPLNPKANREKMTQIMFETFN 421 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Schizosaccharomyces pombe|chr 3|||Manual Length = 791 Score = 25.0 bits (52), Expect = 6.1 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = -2 Query: 300 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 154 WC+ S + SQ+ + +G F PL L WA + + P ITPWW Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 6.1 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 164 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 250 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 >SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 24.6 bits (51), Expect = 8.0 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%) Frame = +2 Query: 71 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 223 N +GM + R F S VG + VG Q+DSY+ D++ S+R Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 24.6 bits (51), Expect = 8.0 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = -1 Query: 268 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 164 GDD+VLD V +D L L AH D H Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,837,545 Number of Sequences: 5004 Number of extensions: 36980 Number of successful extensions: 130 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 188065158 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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