Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31872
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,... 184 3e-47
At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic, p... 173 4e-44
At5g55820.1 68418.m06956 expressed protein 31 0.35
At1g25220.1 68414.m03130 anthranilate synthase beta subunit (ASB... 30 0.80
At1g31460.1 68414.m03852 expressed protein 30 1.1
At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar... 29 1.9
At1g61670.1 68414.m06956 expressed protein similar to membrane p... 29 1.9
At1g64900.1 68414.m07357 cytochrome P450, putative similar to cy... 28 3.2
At4g15380.1 68417.m02350 cytochrome P450 family protein similar ... 28 4.3
At2g12190.1 68415.m01316 cytochrome P450, putative 28 4.3
At1g64950.1 68414.m07362 cytochrome P450, putative similar to cy... 28 4.3
At1g64940.1 68414.m07361 cytochrome P450, putative similar to cy... 28 4.3
At1g24807.1 68414.m03108 anthranilate synthase beta subunit, put... 28 4.3
At5g57890.1 68418.m07242 anthranilate synthase beta subunit, put... 27 5.7
At3g24710.1 68416.m03102 hypothetical protein 27 5.7
At3g07430.1 68416.m00886 YGGT family protein contains Pfam profi... 27 5.7
At5g51380.1 68418.m06370 F-box family protein contains Pfam PF00... 27 7.5
At5g46470.1 68418.m05723 disease resistance protein (TIR-NBS-LRR... 27 7.5
At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 ... 27 7.5
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 27 7.5
At1g08460.1 68414.m00936 histone deacetylase family protein (HDA... 27 7.5
At4g17090.1 68417.m02575 beta-amylase (CT-BMY) / 1,4-alpha-D-glu... 27 9.9
At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase, put... 27 9.9
At1g25155.1 68414.m03123 anthranilate synthase beta subunit, put... 27 9.9
At1g25083.1 68414.m03118 anthranilate synthase beta subunit, put... 27 9.9
At1g24909.1 68414.m03112 anthranilate synthase beta subunit, put... 27 9.9
>At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,
putative similar to Triosephosphate isomerase,
chloroplast precursor: SP|P48496 from Spinacia oleracea,
SP|P46225 from Secale cereale
Length = 315
Score = 184 bits (448), Expect = 3e-47
Identities = 83/158 (52%), Positives = 111/158 (70%)
Frame = +3
Query: 42 KFVVGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVIVGVPAIYLSYVKTIIPDNVEVAAQ 221
KF VGGNWK NG K+ I +++++L L+ +V+V+V P +Y+ VK+ + D ++++ Q
Sbjct: 65 KFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRIDISGQ 124
Query: 222 NCWKSPKGAFTGEISPAMIKDVGVNWVILGHSERRTIFGEKDELVAEKVAHALESGLKVI 401
N W GAFTGEIS +KD+G WVILGHSERR + GEKDE + +K A+AL GL VI
Sbjct: 125 NSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVI 184
Query: 402 ACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIV 515
ACIGE LEERE+GKT +V F QLKA A+ W+NIV
Sbjct: 185 ACIGEKLEEREAGKTFDVCFAQLKAFADAV-PSWDNIV 221
>At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic,
putative strong similarity to triosephosphate isomerase,
cytosolic from Petunia hybrida [SP|P48495], from Coptis
japonica [SP|P21820]
Length = 254
Score = 173 bits (422), Expect = 4e-44
Identities = 84/163 (51%), Positives = 107/163 (65%), Gaps = 2/163 (1%)
Frame = +3
Query: 33 MGRKFVVGGNWKMNGDKNQINEIVNNLKKG--PLDPNVEVIVGVPAIYLSYVKTIIPDNV 206
M RKF VGGNWK NG ++ +IVN L + P VEV+V P ++L VK+ + +
Sbjct: 1 MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDF 60
Query: 207 EVAAQNCWKSPKGAFTGEISPAMIKDVGVNWVILGHSERRTIFGEKDELVAEKVAHALES 386
VAAQNCW GAFTGE+S M+ ++ + WVILGHSERR I E E V +KVA+AL
Sbjct: 61 FVAAQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQ 120
Query: 387 GLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIV 515
GLKVIAC+GETLEERE+G T +VV Q KA+ + W N+V
Sbjct: 121 GLKVIACVGETLEEREAGSTMDVVAAQTKAIADRV-TNWSNVV 162
>At5g55820.1 68418.m06956 expressed protein
Length = 1826
Score = 31.5 bits (68), Expect = 0.35
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = -2
Query: 458 NNFLSLARFSLFESLTNTGNDFQARFKGMSNLFSNKFIFFTKNCSSFRMTKYNPIYSNVF 279
+ +S AR SL E + ND++A G ++ ++K + + S R T P++ + F
Sbjct: 737 HGIISRARSSLIEE--ESANDYKALSDGSNHKSADKQLEVREGNSLLR-TPDRPVFVDNF 793
Query: 278 DHSG*NFTSKSSFWRLPT 225
D N KSS ++PT
Sbjct: 794 DEVPENSREKSSMEKVPT 811
>At1g25220.1 68414.m03130 anthranilate synthase beta subunit (ASB1)
identical to anthranilate synthase beta subunit
GI:403434 from [Arabidopsis thaliana]
Length = 276
Score = 30.3 bits (65), Expect = 0.80
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 221 LGCNFDIIRNDSLHIRQVNSRNTNNYL 141
LGC+F++ RND L + ++ +N L
Sbjct: 95 LGCHFEVYRNDELTVEELKKKNPRGVL 121
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 499 LSPMADTKAFNCLKTTSSVLPDSLSSRVSPIQAMTFKP 386
LS + A NC T S LPD L SR ++++ P
Sbjct: 25 LSDLEQVAAINCSGFTDSTLPDDLESRFRRLKSLPAAP 62
>At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar to
RNA helicase (RH25) [Arabidopsis thaliana] GI:3776023;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain
Length = 798
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = -2
Query: 464 SKNNFLSLARFSLFESLTNTGNDFQARFKGMSNLFSNKF 348
S+ F S++RF ES + G+DF A+ + FS KF
Sbjct: 170 SRRGFSSMSRFKRNESSCDEGDDFDAKKLDTLSPFSPKF 208
>At1g61670.1 68414.m06956 expressed protein similar to membrane
protein PTM1 precursor isolog GI:1931644 from
[Arabidopsis thaliana]
Length = 513
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Frame = +3
Query: 144 VIVGVPAIYLSYVKTIIP--DNVEVAAQNCWKSPKGAFTGEISPAM 275
V++ +Y Y P D + + WK+P G G+++P M
Sbjct: 175 VVINKTGMYYLYFMICDPELDGTRIKGRTVWKNPDGYLPGKVAPLM 220
>At1g64900.1 68414.m07357 cytochrome P450, putative similar to
cytochrome p450 GI:438240 from [Solanum melongena]
Length = 506
Score = 28.3 bits (60), Expect = 3.2
Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = +3
Query: 228 WKSPKGAFTGEI-SPAMIKDVGV--NWVILGHSERRTIFGEKDELVAEKVAHALESGLKV 398
W+ + T EI P+ ++ +WV+ ER G ++ +V H L V
Sbjct: 125 WRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNHGGEEPIVLIHHLHYAMFALLV 184
Query: 399 IACIGETLEERESGKTEEVVFRQLKAL 479
+ C G+ L+E++ + E + QL +L
Sbjct: 185 LMCFGDKLDEKQIKEVEFIQRLQLLSL 211
>At4g15380.1 68417.m02350 cytochrome P450 family protein similar to
CYTOCHROME P450 93A3 (P450 CP5) (SP:O81973) [Glycine
max]
Length = 517
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = -3
Query: 295 FTPTS-LIIAGEISPVKAPFGDFQQF 221
FTPT + AG S + AP+GD+ +F
Sbjct: 112 FTPTDDSLFAGSFSFISAPYGDYWKF 137
>At2g12190.1 68415.m01316 cytochrome P450, putative
Length = 512
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 336 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 470
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 168 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 211
>At1g64950.1 68414.m07362 cytochrome P450, putative similar to
cytochrome P450 89A2 (CYPLXXXIX) (SP:Q42602)
[Arabidopsis thaliana];similar to cytochrome P450
(GI:438242) [Solanum melongena]
Length = 510
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 336 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 470
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 168 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 211
>At1g64940.1 68414.m07361 cytochrome P450, putative similar to
cytochrome p450 GI:438242 from [Solanum melongena]
Length = 511
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = +3
Query: 336 GEKDELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQL 470
GE+ +V + + +A+ + L V+ C G+ L+E++ + E V RQL
Sbjct: 169 GEEPIVVVDHLHYAMFA-LLVLMCFGDKLDEKQIKQVEYVQRRQL 212
>At1g24807.1 68414.m03108 anthranilate synthase beta subunit,
putative similar to anthranilate synthase beta chain
GI:403434; similar to ESTs dbj|AV540153.1,
dbj|AV557490.1, gb|AI997696.1, gb|AW004516.1,
dbj|AV521371.1
Length = 235
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = -2
Query: 263 NFTSKSSFWRLPTVLGCNFDIIRNDSLHIRQVNSRNTNNYL 141
NF + F + L C+F++ RND L + ++ +N L
Sbjct: 40 NFENCYLFLQYMGELQCHFEVYRNDELTVEELKRKNPRGVL 80
>At5g57890.1 68418.m07242 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
chain GI:403434 [Arabidopsis thaliana]
Length = 273
Score = 27.5 bits (58), Expect = 5.7
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 221 LGCNFDIIRNDSLHIRQVNSRNTNNYL 141
LGC+F++ RND L + ++ + L
Sbjct: 92 LGCHFEVYRNDELTVEELKRKKPRGLL 118
>At3g24710.1 68416.m03102 hypothetical protein
Length = 126
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3
Query: 345 DELVAEKVAHALESGLKVIACIGETLEERESGKTEEVVFRQLKALVSA 488
D V + E V++ +GE + E E+G ++EV R+LKAL A
Sbjct: 11 DGAVVYVAGSSTEMPSDVMSALGEIVSE-ETGGSKEVASRRLKALEKA 57
>At3g07430.1 68416.m00886 YGGT family protein contains Pfam profile
PF02325: YGGT family (unknown function)
Length = 232
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -3
Query: 310 PSITQFTPTSLIIAGEISPVKAPFGDFQQFWAATSTLSG 194
PS++ P S+ I+ SP+ P ++ A +STL+G
Sbjct: 44 PSLSIQNPKSIRISASASPITTPILQTEKSTARSSTLTG 82
>At5g51380.1 68418.m06370 F-box family protein contains Pfam
PF00646: F-box domain; similar to F-box protein FBL2
(GI:6063090) [Homo sapiens]
Length = 479
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = -3
Query: 514 TIFSHLSPMADTKAFNCLKTTSSVLPDSLSSRVSPIQAMTFKPDSR 377
++ H + + +C S + +LSS S ++ +T++PD+R
Sbjct: 411 SVILHWEELESMRVVSCKNIKDSEISAALSSLFSLLKELTWRPDTR 456
>At5g46470.1 68418.m05723 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1127
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 224 VLGCNFDIIRNDSLHIRQVNSRNTNNYLNIRI 129
VLG DI D LHI + + + +N L ++I
Sbjct: 533 VLGITLDIDETDELHIHESSFKGMHNLLFLKI 564
>At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 /
alpha-SNAP2 / ASNAP2 identical to alpha-soluble NSF
attachment protein 2 / alpha-SNAP2 SP:Q9SPE6 from
[Arabidopsis thaliana]
Length = 289
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = -1
Query: 441 CQILSLRESHQYRQ*LSSQIQGHEQPFQQQVHLFHQKLFFFQNDQV 304
C+I L + +Y + ++ + +Q F+Q + F + FFQN++V
Sbjct: 103 CEIGRLNMAARYYKEIAEYYES-DQKFEQAIAYFEKAAEFFQNEEV 147
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -1
Query: 459 KQLPQSCQILSLRESHQYRQ*LSSQIQGHEQPFQQQ 352
+Q+PQ Q + Q +Q SQ+Q H+Q QQQ
Sbjct: 712 QQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQ 747
>At1g08460.1 68414.m00936 histone deacetylase family protein (HDA8)
identical to HDA8 [Arabidopsis thaliana] GI:21360988low
similarity to SP|Q9Z2V5 Histone deacetylase 6 (HD6)
(Histone deacetylase mHDA2) {Mus musculus}; contains
Pfam profile PF00850: Histone deacetylase family;
supporting cDNA gi|21360987|gb|AF510167.1|
Length = 377
Score = 27.1 bits (57), Expect = 7.5
Identities = 9/28 (32%), Positives = 20/28 (71%)
Frame = +3
Query: 84 NQINEIVNNLKKGPLDPNVEVIVGVPAI 167
+++ +++ L++GP+ P+V G+PAI
Sbjct: 44 DRVRNMLSILRRGPIAPHVNWFTGLPAI 71
>At4g17090.1 68417.m02575 beta-amylase (CT-BMY) / 1,4-alpha-D-glucan
maltohydrolase identical to beta-amylase enzyme
GI:6065749 from [Arabidopsis thaliana]
Length = 548
Score = 26.6 bits (56), Expect = 9.9
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
Frame = +3
Query: 33 MGRKFVVGGNWKMNGDKNQINEIVNNLKKGPLDPNV--EVIVGVPAIYLSYVKTIIPDNV 206
M ++ G NW+ Q+ E V N+K+G + E G +Y+ +VK I +NV
Sbjct: 490 MNKRLFEGQNWQ------QLVEFVKNMKEGGHGRRLSKEDTTGSD-LYVGFVKGKIAENV 542
Query: 207 EVAA 218
E AA
Sbjct: 543 EEAA 546
>At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase,
putative similar to glycerol-3-phosphate dehydrogenase
GB:BAA08926 from Mus musculus [SP|Q64521], Homo sapiens
[GI:1020315], Rattus norvegicus [SP|P35571]; contains
Pfam profile PF01266 FAD dependent oxidoreductase
Length = 629
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 384 SGLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKW 503
SG++ +A + T + ES + VVF + LV+ G KW
Sbjct: 401 SGIRPLA-MDPTAKSTESISRDHVVFEENPGLVTITGGKW 439
>At1g25155.1 68414.m03123 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
subunit GI:403434 from (Arabidopsis thaliana)
Length = 222
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 221 LGCNFDIIRNDSLHIRQVNSRNTNNYL 141
L C+F++ RND L + ++ +N L
Sbjct: 41 LQCHFEVYRNDELTVEELKRKNPRGVL 67
>At1g25083.1 68414.m03118 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
subunit GI:403434 from (Arabidopsis thaliana); similar
to ESTs dbj|AV540153.1, dbj|AV557490.1, gb|AI997696.1,
gb|AW004516.1, dbj|AV521371.1
Length = 222
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 221 LGCNFDIIRNDSLHIRQVNSRNTNNYL 141
L C+F++ RND L + ++ +N L
Sbjct: 41 LQCHFEVYRNDELTVEELKRKNPRGVL 67
>At1g24909.1 68414.m03112 anthranilate synthase beta subunit,
putative strong similarity to anthranilate synthase beta
subunit GI:403434 from (Arabidopsis thaliana)
Length = 222
Score = 26.6 bits (56), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -2
Query: 221 LGCNFDIIRNDSLHIRQVNSRNTNNYL 141
L C+F++ RND L + ++ +N L
Sbjct: 41 LQCHFEVYRNDELTVEELKRKNPRGVL 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,887,186
Number of Sequences: 28952
Number of extensions: 218071
Number of successful extensions: 649
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 648
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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