BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31857 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g05100.1 68416.m00554 expressed protein 27 7.5 At1g21710.1 68414.m02718 8-oxoguanine-DNA glycosylase (OGG1) sim... 27 7.5 At5g10340.1 68418.m01199 F-box protein-related / SLF-related con... 27 9.9 At4g04885.1 68417.m00711 pre-mRNA cleavage complex-related conta... 27 9.9 >At3g05100.1 68416.m00554 expressed protein Length = 336 Score = 27.1 bits (57), Expect = 7.5 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = -2 Query: 338 INFVTGIFRLSSSSASDCSVHGDGICGTAYNAIEVSV 228 ++FV +F L SSS S CS + + + +A+E SV Sbjct: 27 VSFVAAVFFLLSSSFSSCSC-PEALSSSPTDAVEASV 62 >At1g21710.1 68414.m02718 8-oxoguanine-DNA glycosylase (OGG1) similar to SP|O15527 N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase (EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18) {Homo sapiens}; contains Pfam profiles PF00633: Helix-hairpin-helix motif and PF00730: HhH-GPD superfamily base excision DNA repair protein; identical to cDNA 8-oxoguanine-DNA glycosylase (OGG1 gene) GI:16074116 Length = 365 Score = 27.1 bits (57), Expect = 7.5 Identities = 19/54 (35%), Positives = 25/54 (46%) Frame = +1 Query: 124 DFFNFRNYFVCFHRL*RVSIQDWKY*LYGSSTSYATDTSMALYAVPQIPSPWTL 285 D F F F RL RVS ++++ +G Y T T AL A P + W L Sbjct: 186 DGFEFHQ-FPSLDRLSRVSEEEFRKAGFGYRAKYITGTVNALQAKPGGGNEWLL 238 >At5g10340.1 68418.m01199 F-box protein-related / SLF-related contains weak hit to Pfam Pfam PF00646: F-box domain and to TIGRFAM TIGR01640 : F-box protein interaction domain; weakly similar to self-incompatibility (S-) locus F-box (GI:29420811) [Prunus mume] Length = 445 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 2/26 (7%) Frame = +3 Query: 183 SGLEILTIW--KLYVVCNRYLNGIVC 254 + ++I T W KLY VCN +G++C Sbjct: 157 ASVQIPTPWEDKLYFVCNTSCDGLIC 182 >At4g04885.1 68417.m00711 pre-mRNA cleavage complex-related contains weak similarity to Pre-mRNA cleavage complex II protein Pcf11 (Fragment) (Swiss-Prot:O94913) [Homo sapiens] Length = 808 Score = 26.6 bits (56), Expect = 9.9 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +2 Query: 230 QIPQWHCMRYRRFHPHGHCSRWHL 301 ++P+ C+ YR+ HP H S HL Sbjct: 142 RLPEVFCLAYRQAHPSLHPSMRHL 165 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,849,321 Number of Sequences: 28952 Number of extensions: 185719 Number of successful extensions: 385 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 384 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 385 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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