SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31856
         (443 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   265   2e-72
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   145   3e-36
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   128   3e-31
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   103   2e-23
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    83   1e-17
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    81   1e-16
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    69   3e-13
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    34   0.009
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    31   0.060
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.080
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    29   0.32 
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   2.3  
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch...    25   5.2  
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo...    25   5.2  
SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   6.9  
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch...    25   6.9  
SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|...    24   9.1  
SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma...    24   9.1  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    24   9.1  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  265 bits (650), Expect = 2e-72
 Identities = 116/129 (89%), Positives = 125/129 (96%)
 Frame = +2

Query: 56  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 235
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 236 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 415
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 416 IMFETFNXP 442
           I+FETFN P
Sbjct: 122 IIFETFNAP 130


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  145 bits (351), Expect = 3e-36
 Identities = 66/128 (51%), Positives = 88/128 (68%), Gaps = 1/128 (0%)
 Frame = +2

Query: 62  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 241
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 242 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 418
           +L ++ PIE GII NW DME+IW + + + +L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 419 MFETFNXP 442
            +ET N P
Sbjct: 128 FYETLNVP 135


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  128 bits (310), Expect = 3e-31
 Identities = 59/124 (47%), Positives = 87/124 (70%), Gaps = 2/124 (1%)
 Frame = +2

Query: 68  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 241
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 242 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 421
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 422 FETF 433
           FE +
Sbjct: 124 FERY 127


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  103 bits (246), Expect = 2e-23
 Identities = 58/145 (40%), Positives = 78/145 (53%), Gaps = 24/145 (16%)
 Frame = +2

Query: 74  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 202
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 203 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 361
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 362 LLTEAPLNPKANREKMTQIMFETFN 436
           LLTE PLNP  NRE   +IMFE+FN
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFN 151


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 83.4 bits (197), Expect = 1e-17
 Identities = 45/134 (33%), Positives = 78/134 (58%), Gaps = 1/134 (0%)
 Frame = +2

Query: 44  FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 223
           F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +     + +  + VG++
Sbjct: 18  FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72

Query: 224 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 400
              + G  ++ + P E  +I+NWD ME++  +TF  +L +   EHP+ +TE   NP   R
Sbjct: 73  TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131

Query: 401 EKMTQIMFETFNXP 442
             MT+++FE +N P
Sbjct: 132 STMTELLFELYNAP 145


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 80.6 bits (190), Expect = 1e-16
 Identities = 44/128 (34%), Positives = 68/128 (53%)
 Frame = +2

Query: 59  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 238
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 239 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 418
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 419 MFETFNXP 442
           MFE+   P
Sbjct: 121 MFESLRCP 128


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 68.9 bits (161), Expect = 3e-13
 Identities = 37/130 (28%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
 Frame = +2

Query: 59  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 238
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 239 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 412
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 413 QIMFETFNXP 442
           +  FE  + P
Sbjct: 122 EAAFEQLHVP 131


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 34.3 bits (75), Expect = 0.009
 Identities = 30/124 (24%), Positives = 54/124 (43%), Gaps = 4/124 (3%)
 Frame = +2

Query: 74  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 253
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 254 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 421
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 422 FETF 433
           FE F
Sbjct: 118 FEEF 121


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 31.5 bits (68), Expect = 0.060
 Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 4/65 (6%)
 Frame = +2

Query: 260 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 427
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 428 TFNXP 442
               P
Sbjct: 172 ECQVP 176


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.080
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +2

Query: 119 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 262
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 29.1 bits (62), Expect = 0.32
 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
 Frame = +2

Query: 320  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNXP 442
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 2.3
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +2

Query: 362 LLTEAPLNPKANREKMTQIMFETFN 436
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
           family|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 791

 Score = 25.0 bits (52), Expect = 5.2
 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
 Frame = -3

Query: 315 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 169
           WC+  S + SQ+  +   +G F    PL L WA   +  +    P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346


>SPBC1683.09c |frp1||ferric-chelate reductase
           Frp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 564

 Score = 25.0 bits (52), Expect = 5.2
 Identities = 11/29 (37%), Positives = 17/29 (58%)
 Frame = +2

Query: 179 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 265
           +M+G     +Y+G  AQ KR +LT +  I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193


>SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 485

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%)
 Frame = +2

Query: 86  NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 238
           N +GM         + R  F S VG  +     VG       Q+DSY+ D++ S+R
Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373


>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
           Y|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1002

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 14/35 (40%), Positives = 16/35 (45%)
 Frame = -2

Query: 283 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 179
           GDD+VLD V   +D    L L        AH D H
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710


>SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 341

 Score = 24.2 bits (50), Expect = 9.1
 Identities = 12/41 (29%), Positives = 26/41 (63%), Gaps = 3/41 (7%)
 Frame = +2

Query: 275 IITNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAPLNP 388
           +I + DD    +W+  HTF+++ +  P EH + ++++ +NP
Sbjct: 271 VIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310


>SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 319

 Score = 24.2 bits (50), Expect = 9.1
 Identities = 8/31 (25%), Positives = 17/31 (54%)
 Frame = +2

Query: 257 YPIEHGIITNWDDMEKIWHHTFYNELRVAPE 349
           YP   G++ +W ++E +       E+R+ P+
Sbjct: 244 YPRTDGLLFSWSELESMNSENMKPEIRLIPK 274


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 24.2 bits (50), Expect = 9.1
 Identities = 10/26 (38%), Positives = 15/26 (57%)
 Frame = -3

Query: 162  RPTMDGKTARGASSPAKPALHMPEPL 85
            RP+ DG+ A G    A P++  P P+
Sbjct: 950  RPSNDGRKASGPRPAAPPSIPPPLPV 975


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,658,836
Number of Sequences: 5004
Number of extensions: 33350
Number of successful extensions: 119
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 162176800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -