BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31856 (443 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 265 2e-72 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 145 3e-36 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 128 3e-31 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 103 2e-23 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 83 1e-17 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 81 1e-16 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 69 3e-13 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 34 0.009 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 31 0.060 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.080 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.32 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.3 SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 5.2 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 5.2 SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 6.9 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 6.9 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 24 9.1 SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 24 9.1 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 24 9.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 265 bits (650), Expect = 2e-72 Identities = 116/129 (89%), Positives = 125/129 (96%) Frame = +2 Query: 56 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 235 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 236 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 415 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 416 IMFETFNXP 442 I+FETFN P Sbjct: 122 IIFETFNAP 130 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 145 bits (351), Expect = 3e-36 Identities = 66/128 (51%), Positives = 88/128 (68%), Gaps = 1/128 (0%) Frame = +2 Query: 62 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 241 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 242 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 418 +L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 419 MFETFNXP 442 +ET N P Sbjct: 128 FYETLNVP 135 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 128 bits (310), Expect = 3e-31 Identities = 59/124 (47%), Positives = 87/124 (70%), Gaps = 2/124 (1%) Frame = +2 Query: 68 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 241 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 242 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 421 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 422 FETF 433 FE + Sbjct: 124 FERY 127 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 103 bits (246), Expect = 2e-23 Identities = 58/145 (40%), Positives = 78/145 (53%), Gaps = 24/145 (16%) Frame = +2 Query: 74 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 202 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 203 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 361 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 362 LLTEAPLNPKANREKMTQIMFETFN 436 LLTE PLNP NRE +IMFE+FN Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFN 151 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 83.4 bits (197), Expect = 1e-17 Identities = 45/134 (33%), Positives = 78/134 (58%), Gaps = 1/134 (0%) Frame = +2 Query: 44 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 223 F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72 Query: 224 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 400 + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +TE NP R Sbjct: 73 TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131 Query: 401 EKMTQIMFETFNXP 442 MT+++FE +N P Sbjct: 132 STMTELLFELYNAP 145 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 80.6 bits (190), Expect = 1e-16 Identities = 44/128 (34%), Positives = 68/128 (53%) Frame = +2 Query: 59 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 238 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 239 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 418 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 419 MFETFNXP 442 MFE+ P Sbjct: 121 MFESLRCP 128 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 68.9 bits (161), Expect = 3e-13 Identities = 37/130 (28%), Positives = 70/130 (53%), Gaps = 2/130 (1%) Frame = +2 Query: 59 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 238 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 239 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 412 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 413 QIMFETFNXP 442 + FE + P Sbjct: 122 EAAFEQLHVP 131 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 34.3 bits (75), Expect = 0.009 Identities = 30/124 (24%), Positives = 54/124 (43%), Gaps = 4/124 (3%) Frame = +2 Query: 74 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 253 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 254 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 421 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 422 FETF 433 FE F Sbjct: 118 FEEF 121 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 31.5 bits (68), Expect = 0.060 Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 4/65 (6%) Frame = +2 Query: 260 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 427 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 428 TFNXP 442 P Sbjct: 172 ECQVP 176 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.080 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 119 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 262 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 29.1 bits (62), Expect = 0.32 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +2 Query: 320 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNXP 442 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N P Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 2.3 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 362 LLTEAPLNPKANREKMTQIMFETFN 436 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Schizosaccharomyces pombe|chr 3|||Manual Length = 791 Score = 25.0 bits (52), Expect = 5.2 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = -3 Query: 315 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 169 WC+ S + SQ+ + +G F PL L WA + + P ITPWW Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 5.2 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 179 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 265 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 >SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 24.6 bits (51), Expect = 6.9 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%) Frame = +2 Query: 86 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 238 N +GM + R F S VG + VG Q+DSY+ D++ S+R Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 24.6 bits (51), Expect = 6.9 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = -2 Query: 283 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 179 GDD+VLD V +D L L AH D H Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 24.2 bits (50), Expect = 9.1 Identities = 12/41 (29%), Positives = 26/41 (63%), Gaps = 3/41 (7%) Frame = +2 Query: 275 IITNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAPLNP 388 +I + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 271 VIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 >SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 24.2 bits (50), Expect = 9.1 Identities = 8/31 (25%), Positives = 17/31 (54%) Frame = +2 Query: 257 YPIEHGIITNWDDMEKIWHHTFYNELRVAPE 349 YP G++ +W ++E + E+R+ P+ Sbjct: 244 YPRTDGLLFSWSELESMNSENMKPEIRLIPK 274 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 24.2 bits (50), Expect = 9.1 Identities = 10/26 (38%), Positives = 15/26 (57%) Frame = -3 Query: 162 RPTMDGKTARGASSPAKPALHMPEPL 85 RP+ DG+ A G A P++ P P+ Sbjct: 950 RPSNDGRKASGPRPAAPPSIPPPLPV 975 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,658,836 Number of Sequences: 5004 Number of extensions: 33350 Number of successful extensions: 119 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 162176800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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