Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31828
(506 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 81 9e-17
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 62 5e-11
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 52 4e-08
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 46 3e-06
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 30 0.23
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 1.6
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 27 2.1
SPAC3G9.14 |sak1||transcriptional repressor Sak1|Schizosaccharom... 26 2.8
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 26 3.7
SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizos... 25 4.9
SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 25 6.5
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 25 6.5
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 81.0 bits (191), Expect = 9e-17
Identities = 42/152 (27%), Positives = 62/152 (40%), Gaps = 1/152 (0%)
Frame = +2
Query: 20 KCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNEPWHRE 199
+CF C C + F RE + YC Y ++F+ RC C I V N+ +H
Sbjct: 281 QCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHEN 340
Query: 200 CFTCTNCNTSL-AGQRFTSRDEKPYCAECFGELFAKRCTSCTKPITGIGGTRFISFEDRH 376
C C+ RD+ +C C+ +A +C C KPI GI + D
Sbjct: 341 HHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPILGIS----VKGSDGE 396
Query: 377 WHNDCFICAQCKTSLVGKGFITDGQDIICPEC 472
+H+ C+ C C L +G+ IC C
Sbjct: 397 YHSQCWTCGACNALLGDEGYFMIENTPICRPC 428
Score = 45.6 bits (103), Expect = 4e-06
Identities = 26/102 (25%), Positives = 41/102 (40%), Gaps = 1/102 (0%)
Frame = +2
Query: 107 YEDKFATRCVKCNKIITQGGV-TYKNEPWHRECFTCTNCNTSLAGQRFTSRDEKPYCAEC 283
Y C C + G + + + H +CF C C+ +L F R+ K YC
Sbjct: 250 YRGNSEKSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLD 309
Query: 284 FGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQC 409
+ E F+ RC C PI + + + +H + CA C
Sbjct: 310 YHEQFSPRCKHCKTPIE----DQAVHINNDWFHENHHFCAGC 347
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 62.1 bits (144), Expect = 5e-11
Identities = 34/124 (27%), Positives = 57/124 (45%), Gaps = 5/124 (4%)
Frame = +2
Query: 116 KFATRCVKCNKIIT--QGGVTYKNEPWHRECFTCTNCNTSL--AGQRFT-SRDEKPYCAE 280
K +T C +C + + +++ WH++CF CT C+ L + Q + D +P C+
Sbjct: 14 KGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSS 73
Query: 281 CFGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSLVGKGFITDGQDII 460
C A CT+C I +S D + H +CF C C+ ++ F D + I
Sbjct: 74 C-----AHTCTACRMRIKDYA---LMSGYDSY-HRECFRCHDCRKQIIDSNFKRDNRTIF 124
Query: 461 CPEC 472
C +C
Sbjct: 125 CNDC 128
Score = 40.7 bits (91), Expect = 1e-04
Identities = 21/90 (23%), Positives = 33/90 (36%), Gaps = 3/90 (3%)
Frame = +2
Query: 23 CFCCVVCKNPIGTKSFI---PREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNEPWH 193
CFCC C + + + C+ C A C C I + + +H
Sbjct: 44 CFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIKDYALMSGYDSYH 98
Query: 194 RECFTCTNCNTSLAGQRFTSRDEKPYCAEC 283
RECF C +C + F + +C +C
Sbjct: 99 RECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 52.4 bits (120), Expect = 4e-08
Identities = 34/130 (26%), Positives = 52/130 (40%), Gaps = 6/130 (4%)
Frame = +2
Query: 20 KCFCCVVCKNPIGTKSF-----IPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNE 184
+CF C C + + +K F +Q C Y + C C + +T N+
Sbjct: 138 ECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCGMALRGYYITALNK 197
Query: 185 PWHRECFTCTNCNTSLA-GQRFTSRDEKPYCAECFGELFAKRCTSCTKPITGIGGTRFIS 361
+H E FTC+ C T + + K YC + LFA RC C PI + +
Sbjct: 198 KFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRN 257
Query: 362 FEDRHWHNDC 391
++WH C
Sbjct: 258 GVSQNWHVPC 267
Score = 44.8 bits (101), Expect = 8e-06
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 5/100 (5%)
Frame = +2
Query: 131 CVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSLAGQRF-----TSRDEKPYCAECFGEL 295
C C ++I+ V +H ECF C +CN+ +A + F T + P C +
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175
Query: 296 FAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKT 415
C SC + G +I+ ++ +H + F C+ C T
Sbjct: 176 LDLLCASCGMALRGY----YITALNKKFHIEHFTCSLCYT 211
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 46.0 bits (104), Expect = 3e-06
Identities = 32/121 (26%), Positives = 52/121 (42%), Gaps = 4/121 (3%)
Frame = +2
Query: 131 CVKC-NKIITQGGVTYKNEPWHRECFTCTNCNTSL--AGQRFTSRDEKPYCAECFGELFA 301
C+KC + + V + + WH +CF C NCN L + + F+ D+K + F +L
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQK----QIFCKLCV 79
Query: 302 KRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSLVGKGF-ITDGQDIICPECAK 478
C C+ PI F + + ++ C C+ C+ + F GQ P K
Sbjct: 80 DICNGCSTPI-----CEFNAVSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRK 132
Query: 479 Q 481
Q
Sbjct: 133 Q 133
Score = 35.1 bits (77), Expect = 0.006
Identities = 19/61 (31%), Positives = 26/61 (42%), Gaps = 3/61 (4%)
Frame = +2
Query: 299 AKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSL--VGKGFITDGQ-DIICPE 469
++R C K + T + F + WH+DCF C C L + F D Q I C
Sbjct: 18 SERTCFCIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKL 77
Query: 470 C 472
C
Sbjct: 78 C 78
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 29.9 bits (64), Expect = 0.23
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2
Query: 374 HWHNDCFICAQCKTSLVGKGFI 439
H + C++CA+CKT L KG +
Sbjct: 8 HLKSRCYVCAKCKTHLAFKGHL 29
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.1 bits (57), Expect = 1.6
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +2
Query: 203 FTCTNCNTSLAGQRFTSRDEKPYCAEC 283
FTC C + L GQ++ S A+C
Sbjct: 625 FTCQECMSMLVGQKYGSSSTSTIIAKC 651
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 26.6 bits (56), Expect = 2.1
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = -3
Query: 432 PLPTSEVLHCAQMKQSLCQ-CLSSNEMKRVPPIPVMGLVQEVQRLANSSPKHSAQ*GFSS 256
P P+SEV C +Q + + L +N + P +Q RL N + + F +
Sbjct: 357 PFPSSEVFRCLATRQEILKGMLGANILGFQIPEFAYHFLQTCSRLVNIDIRKNGVVSFEN 416
Query: 255 REVN 244
R+++
Sbjct: 417 RQID 420
>SPAC3G9.14 |sak1||transcriptional repressor
Sak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 766
Score = 26.2 bits (55), Expect = 2.8
Identities = 22/97 (22%), Positives = 42/97 (43%), Gaps = 3/97 (3%)
Frame = -3
Query: 399 QMKQSLCQCLSSNEMKRVPPIPVMGLVQEVQRLAN--SSPKHSAQ*GFSSREVNRW-PAS 229
+ K +L SS+ + + + M L Q + ++N +S S SS +W S
Sbjct: 388 EAKSALMNIYSSHCITLIESVRYMHLKQFLSEISNFPNSLSPSLLALLSSPYFTKWIERS 447
Query: 228 DVLQFVHVKHSRCHGSFLYVTPPCVIILLHLTQRVAN 118
D + + + + V PP +++L HL + + N
Sbjct: 448 DTVMYREILKLLFPMTLQVVPPPVLVLLRHLAENLVN 484
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 25.8 bits (54), Expect = 3.7
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = +2
Query: 206 TCTNCNTSLAGQRFTSRDEKPYCAEC 283
TCTNC T S D +P C C
Sbjct: 634 TCTNCQTRTTPLWRRSPDGQPLCNAC 659
>SPCC1020.10 |oca2||serine/threonine protein kinase Oca2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 650
Score = 25.4 bits (53), Expect = 4.9
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +1
Query: 409 QDLARRQGLHHRRTGHH 459
Q + R+ GLHH + GHH
Sbjct: 213 QFIFRKLGLHHGKHGHH 229
>SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr
1|||Manual
Length = 113
Score = 25.0 bits (52), Expect = 6.5
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = +2
Query: 353 FISFEDRHWHNDCFICAQCKTSL 421
F+ + +H C IC CK SL
Sbjct: 58 FLPWRKAKFHIPCVICGACKNSL 80
>SPCC777.14 |prp4||serine/threonine protein kinase
Prp4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 477
Score = 25.0 bits (52), Expect = 6.5
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
Frame = -3
Query: 444 SVMKPLPTS---EVLHCAQMKQSLCQCLSSNEMKRVPP 340
S +K +PTS E + ++ L +CL N KRVPP
Sbjct: 428 SRLKEVPTSTDEEFIIRQELMDLLEKCLELNPEKRVPP 465
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,574,158
Number of Sequences: 5004
Number of extensions: 29683
Number of successful extensions: 114
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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