Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31815
(418 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 59 3e-10
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 56 2e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 2e-05
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 1.2
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 1.2
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 27 1.5
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 27 1.5
SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 26 2.0
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 3.6
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 25 4.7
SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pom... 25 6.2
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 25 6.2
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 24 8.2
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 24 8.2
SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces po... 24 8.2
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 58.8 bits (136), Expect = 3e-10
Identities = 35/103 (33%), Positives = 54/103 (52%)
Frame = +2
Query: 107 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 286
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 287 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 415
G E+F++ +++DK+ G++ EL + L +LGEKL
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKL 110
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 56.4 bits (130), Expect = 2e-09
Identities = 38/120 (31%), Positives = 60/120 (50%), Gaps = 3/120 (2%)
Frame = +2
Query: 65 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 238
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 239 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 415
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 42.7 bits (96), Expect = 2e-05
Identities = 27/113 (23%), Positives = 52/113 (46%)
Frame = +2
Query: 77 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 256
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 257 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 415
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKL 111
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.1 bits (57), Expect = 1.2
Identities = 31/122 (25%), Positives = 50/122 (40%)
Frame = +3
Query: 24 SVPSRSSLADVQTK*ATSARTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQ 203
S+ S SSLA T TSA T S S +++ A + S+LA S S+
Sbjct: 84 SLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSLASSSTTSSSLASSSITSSSLASSS 143
Query: 204 PSRNSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKISWNV*SCMTRTKTVSCLALSX 383
+ +S+ S S + SS ST T + +S S + +++ +L+
Sbjct: 144 ITSSSLAS-SSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSATSSSLASSSLNS 202
Query: 384 HT 389
T
Sbjct: 203 TT 204
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 1.2
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 65 MSDLSKNDVER-ASFAFSIYDFEGKGKID 148
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 26.6 bits (56), Expect = 1.5
Identities = 24/69 (34%), Positives = 32/69 (46%), Gaps = 2/69 (2%)
Frame = -2
Query: 204 VASVGFELSALRRSPRLKASILPLPSKS*IEKAKDALSTSFLLRSLILSERLPVKTGR-- 31
V S GF SA + A + PS++ E K+AL FL IL E L +T +
Sbjct: 77 VISTGFAWSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKIL-ENLDEQTRKLS 135
Query: 30 EPTNTTQNE 4
E T Q+E
Sbjct: 136 ERTEKQQDE 144
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 26.6 bits (56), Expect = 1.5
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +2
Query: 74 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 190
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 630
Score = 26.2 bits (55), Expect = 2.0
Identities = 23/81 (28%), Positives = 37/81 (45%)
Frame = +3
Query: 21 LSVPSRSSLADVQTK*ATSARTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHW 200
LSV S S +A +T AT ++ R + + + A + PS I + +TP+
Sbjct: 347 LSV-SNSKVASPETPNATHTAPKVEMRYASAAAAAAAALAKESPSHHYIMQQVRPETPN- 404
Query: 201 QPSRNSVVQRRRARSCSHSKS 263
P NS V + + S H+ S
Sbjct: 405 SPRLNSTVIQSKWDSLGHTAS 425
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 3.6
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +3
Query: 84 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 257
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 258 KSSFPSTAKQRKTKT 302
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting
protein 3 homolog Bud6|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1385
Score = 25.0 bits (52), Expect = 4.7
Identities = 17/60 (28%), Positives = 30/60 (50%)
Frame = +3
Query: 30 PSRSSLADVQTK*ATSARTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPS 209
P +AD ATS R L+ ++ ++S+ +++ S+ L++ STQ QPS
Sbjct: 482 PKLEDIADEVETDATSQRELLERQIQKAESSEDTSEIESLQGKLSLPQVSSTQ-QEIQPS 540
>SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 561
Score = 24.6 bits (51), Expect = 6.2
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2
Query: 405 PSARSVCVSSAPSMRPFSFLSYSFRHSRKSSYAP 304
PSA S+ A + LS + R SR+S Y+P
Sbjct: 382 PSAPSLSKGRAMTADNMDMLSLTTRRSRRSLYSP 415
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1254
Score = 24.6 bits (51), Expect = 6.2
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = -2
Query: 138 PLPSKS*IEKAKDALSTSFLLRSLILSERLPVKTGREPTNTTQN 7
P+P K +++ ++L S+ L S I S V PT + +N
Sbjct: 273 PIPKKESRKESSNSLYESYRLGSQIASPSPSVSGSASPTKSNKN 316
>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 24.2 bits (50), Expect = 8.2
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 89 VERASFAFSIYDFEGKGKIDAFNLG 163
+E A SIYDF +I+A N+G
Sbjct: 117 IEDAPPTQSIYDFSSSRQINALNVG 141
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 24.2 bits (50), Expect = 8.2
Identities = 19/75 (25%), Positives = 36/75 (48%)
Frame = +3
Query: 84 TTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKS 263
++L +L+P T KAK S+ S S S+ + W + V++ A S S++
Sbjct: 348 SSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SPSEA 404
Query: 264 SFPSTAKQRKTKTRE 308
S P+ +K + ++
Sbjct: 405 SLPNLSKYSRKNAKK 419
>SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 399
Score = 24.2 bits (50), Expect = 8.2
Identities = 14/38 (36%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
Frame = +3
Query: 216 SVVQRRRARS-CSHSKSSFPSTAKQRKTKTRERMKISW 326
S+ R A S SHS +T ++ KT RER+ W
Sbjct: 294 SLAMRSLAHSGTSHSLDLHGATVREAKTIVRERVAAWW 331
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,626,242
Number of Sequences: 5004
Number of extensions: 28380
Number of successful extensions: 114
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 146319408
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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