Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31794
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g39420.1 68418.m04775 protein kinase family protein contains ... 35 0.037
At5g44310.2 68418.m05424 late embryogenesis abundant domain-cont... 34 0.065
At5g44310.1 68418.m05423 late embryogenesis abundant domain-cont... 34 0.065
At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 tr... 30 0.80
At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 tr... 30 0.80
At4g27595.1 68417.m03964 protein transport protein-related low s... 29 1.9
At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi... 29 2.5
At4g32160.1 68417.m04574 phox (PX) domain-containing protein con... 28 3.2
At5g20440.1 68418.m02430 mob1/phocein family protein contains Pf... 28 4.3
At5g46070.1 68418.m05665 guanylate-binding family protein contai... 27 5.7
At5g07780.1 68418.m00890 formin homology 2 domain-containing pro... 27 5.7
At4g36120.1 68417.m05141 expressed protein 27 5.7
At3g50100.1 68416.m05477 exonuclease family protein contains exo... 27 5.7
At3g10730.1 68416.m01292 sad1/unc-84-like 2 family protein conta... 27 5.7
At5g04990.1 68418.m00528 sad1/unc-84 protein-related contains we... 27 7.5
At2g41640.1 68415.m05145 expressed protein contains Pfam domain,... 27 7.5
At2g06750.1 68415.m00753 hypothetical protein similar to At5g282... 27 7.5
At5g51370.1 68418.m06369 F-box family protein similar to unknown... 27 9.9
At2g32240.1 68415.m03940 expressed protein contains Pfam profile... 27 9.9
>At5g39420.1 68418.m04775 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 34.7 bits (76), Expect = 0.037
Identities = 24/87 (27%), Positives = 42/87 (48%)
Frame = +3
Query: 144 PHQGSSIRTLEQQFNSLTKSKDAQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXK 323
P S + + ++ SL S DFS+ +D + L+ L +S+ G L +GK
Sbjct: 561 PSHESKLSRIGERHGSLDGS--GLDFSQREEDSPKKTLEHLQFGKQSISGPLIFKSGKID 618
Query: 324 EALEQSRQNIERTAEELRKGHPDVEKN 404
E L+++ NI + +RK H E++
Sbjct: 619 EILQRNESNIR---QAVRKSHLQREQD 642
>At5g44310.2 68418.m05424 late embryogenesis abundant
domain-containing protein / LEA domain-containing
protein low similarity to 51 kDa seed maturation protein
[Glycine max] GI:414977; contains Pfam profile PF02987:
Late embryogenesis abundant protein
Length = 331
Score = 33.9 bits (74), Expect = 0.065
Identities = 27/127 (21%), Positives = 56/127 (44%), Gaps = 5/127 (3%)
Frame = +3
Query: 147 HQGSSIRTLEQQFNSLTKSKD-AQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXK 323
++G+S R ++ + + K+KD A D + KD E ++N A + K K
Sbjct: 116 NEGAS-RAADKAYETKEKAKDKAYDVKEKTKDYAEEAKDKVNEGASRAADKAYETKEKAK 174
Query: 324 EAL----EQSRQNIERTAEELRKGHPDVEKNATALREKLQAAVQNTVQESHKLXXKVXSN 491
+ E+++ E T E++ +G A ++EK + + T + ++ +
Sbjct: 175 DKAYDVKEKTKDFAEETKEKVNEGASRAADKAYDVKEKTKNYAEQTKDKVNEGASRAADK 234
Query: 492 VQETNEK 512
+ET +K
Sbjct: 235 AEETKDK 241
>At5g44310.1 68418.m05423 late embryogenesis abundant
domain-containing protein / LEA domain-containing
protein low similarity to 51 kDa seed maturation protein
[Glycine max] GI:414977; contains Pfam profile PF02987:
Late embryogenesis abundant protein
Length = 295
Score = 33.9 bits (74), Expect = 0.065
Identities = 27/127 (21%), Positives = 56/127 (44%), Gaps = 5/127 (3%)
Frame = +3
Query: 147 HQGSSIRTLEQQFNSLTKSKD-AQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXK 323
++G+S R ++ + + K+KD A D + KD E ++N A + K K
Sbjct: 80 NEGAS-RAADKAYETKEKAKDKAYDVKEKTKDYAEEAKDKVNEGASRAADKAYETKEKAK 138
Query: 324 EAL----EQSRQNIERTAEELRKGHPDVEKNATALREKLQAAVQNTVQESHKLXXKVXSN 491
+ E+++ E T E++ +G A ++EK + + T + ++ +
Sbjct: 139 DKAYDVKEKTKDFAEETKEKVNEGASRAADKAYDVKEKTKNYAEQTKDKVNEGASRAADK 198
Query: 492 VQETNEK 512
+ET +K
Sbjct: 199 AEETKDK 205
>At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2
transmembrane domains; similar to DNA double-strand
break repair rad50 ATPase. (Swiss-Prot:O33600)
[Sulfolobus acidocaldarius]
Length = 440
Score = 30.3 bits (65), Expect = 0.80
Identities = 20/73 (27%), Positives = 34/73 (46%)
Frame = +3
Query: 207 DAQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXKEALEQSRQNIERTAEELRKGH 386
D D K DG + L QLNA ++L+ + D K + E+ E+ +E +
Sbjct: 30 DGGDEPKLRSDGGDIELDQLNAKIRALESQIDDKT-KELKGREELVTEKEKLLQERQDKV 88
Query: 387 PDVEKNATALREK 425
+E ++LR+K
Sbjct: 89 ASLETEVSSLRKK 101
>At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2
transmembrane domains; similar to DNA double-strand
break repair rad50 ATPase. (Swiss-Prot:O33600)
[Sulfolobus acidocaldarius]
Length = 440
Score = 30.3 bits (65), Expect = 0.80
Identities = 20/73 (27%), Positives = 34/73 (46%)
Frame = +3
Query: 207 DAQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXKEALEQSRQNIERTAEELRKGH 386
D D K DG + L QLNA ++L+ + D K + E+ E+ +E +
Sbjct: 30 DGGDEPKLRSDGGDIELDQLNAKIRALESQIDDKT-KELKGREELVTEKEKLLQERQDKV 88
Query: 387 PDVEKNATALREK 425
+E ++LR+K
Sbjct: 89 ASLETEVSSLRKK 101
>At4g27595.1 68417.m03964 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 1212
Score = 29.1 bits (62), Expect = 1.9
Identities = 28/72 (38%), Positives = 35/72 (48%), Gaps = 3/72 (4%)
Frame = +3
Query: 303 DXNGKXKEAL---EQSRQNIERTAEELRKGHPDVEKNATALREKLQAAVQNTVQESHKLX 473
D K KE+L E QNI + AEELR D K E+L AA ++ V++ KL
Sbjct: 647 DQLSKVKESLVDKETKLQNIIQEAEELRVKEIDYLKKI----EELSAAKESLVEKETKL- 701
Query: 474 XKVXSNVQETNE 509
S VQE E
Sbjct: 702 ---LSTVQEAEE 710
>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
histidine--tRNA ligase, putative similar to SP|P12081
Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
PF00587: tRNA synthetase class II core domain (G, H, P,
S and T), PF03129: Anticodon binding domain
Length = 479
Score = 28.7 bits (61), Expect = 2.5
Identities = 22/67 (32%), Positives = 29/67 (43%)
Frame = +3
Query: 246 ESVLQQLNAXAKSLQGALGDXNGKXKEALEQSRQNIERTAEELRKGHPDVEKNATALREK 425
E +L N +S L K KE +E N R +L KG D K A+REK
Sbjct: 9 EQILNLFNPEGRSFDSLLD----KVKEIVES---NENRRLPKLPKGTRDFAKEQMAVREK 61
Query: 426 LQAAVQN 446
+ +QN
Sbjct: 62 AFSIIQN 68
>At4g32160.1 68417.m04574 phox (PX) domain-containing protein
contains Pfam profile PF00787: PX domain
Length = 723
Score = 28.3 bits (60), Expect = 3.2
Identities = 25/97 (25%), Positives = 44/97 (45%), Gaps = 10/97 (10%)
Frame = +3
Query: 249 SVLQQLNAXAKSLQGALGDXNGKXKEALEQS------RQN-IERTAEELRKGHPDVEKNA 407
+V LN L+G L + KE LEQ+ R N ++ EELR+ ++E
Sbjct: 423 AVKDYLNKKVNDLEGELETTKQRSKENLEQAIMSERERFNQMQWDMEELRQKSYEMEMKL 482
Query: 408 TALRE---KLQAAVQNTVQESHKLXXKVXSNVQETNE 509
+ + + VQ+T+ E H L ++ + Q+ +
Sbjct: 483 KSREDGSSHAEPTVQSTISEKHVLSKELDARKQQLED 519
>At5g20440.1 68418.m02430 mob1/phocein family protein contains Pfam
profile: PF03637 mob1/phocein family
Length = 217
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = +3
Query: 339 SRQNIERTAEELRKGHPDVEKNATALREKLQAAVQNTVQESHKLXXKVXSN 491
S N+ RT ++ HP+ + + +RE + + ++E+ +L V N
Sbjct: 9 SSNNVVRTVPSKKRKHPEYKSKGSQIRELISGIRSDNLREAVRLPQGVDIN 59
>At5g46070.1 68418.m05665 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding protein,
C-terminal domain
Length = 1060
Score = 27.5 bits (58), Expect = 5.7
Identities = 27/108 (25%), Positives = 44/108 (40%), Gaps = 7/108 (6%)
Frame = +3
Query: 156 SSIRTLEQQFNSLTKSK-DAQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXKEAL 332
+ I E+Q +L + K D +D + + ++ ++ + KE
Sbjct: 809 AQIERAERQVENLERQKTDLEDELDRLRVSEMEAVSKVTILEARVEEREKEIGSLIKETN 868
Query: 333 EQSRQNI---ERTAEELRKGHPDVEKNATALREKLQAA---VQNTVQE 458
Q N+ E+ +E RK H + A AL +LQAA V N QE
Sbjct: 869 AQRAHNVKSLEKLLDEERKAHIAANRRAEALSLELQAAQAHVDNLQQE 916
>At5g07780.1 68418.m00890 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 464
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/35 (37%), Positives = 15/35 (42%)
Frame = -1
Query: 381 PCGAPRPCARCSASTVPKPPXPXRSXLRALPGDSW 277
P PRPC+R + P RALPG W
Sbjct: 61 PPPLPRPCSRPPKTKCSLKPLHWVKKTRALPGSLW 95
>At4g36120.1 68417.m05141 expressed protein
Length = 981
Score = 27.5 bits (58), Expect = 5.7
Identities = 21/87 (24%), Positives = 39/87 (44%)
Frame = +3
Query: 171 LEQQFNSLTKSKDAQDFSKXWKDGXESVLQQLNAXAKSLQGALGDXNGKXKEALEQSRQN 350
LE++ ++K K S+ + E+ L+ + KSL + K K +LE+ +
Sbjct: 775 LEEKEQLISKLKSQLTSSEDLQSLAETQLKCVTESYKSLDLHAKELEAKVK-SLEEETKR 833
Query: 351 IERTAEELRKGHPDVEKNATALREKLQ 431
+E + GH + L+EK+Q
Sbjct: 834 LEMAFTTEKHGHEETLAKCRDLQEKMQ 860
>At3g50100.1 68416.m05477 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 406
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +3
Query: 258 QQLNAXAKSLQGA-LGDXNGKXKEALEQSRQNIERTAEELRKGHPDVEKNATALREK 425
Q L+ K LQ L NG KE L +N + ++ R+ H D+ + T ++K
Sbjct: 11 QVLDELVKLLQSRDLRGENGNWKEFLHVYDKNADSPSDPSRRSHEDLVQFLTTFKKK 67
>At3g10730.1 68416.m01292 sad1/unc-84-like 2 family protein contains
1 transmembrane domain; similar to Sad1 unc-84 domain
protein 2 (GI:6538749) [Homo sapiens]; similar to
Sad1/unc-84-like protein 2 (Fragment)
(Swiss-Prot:Q9UH99) [Homo sapiens]
Length = 455
Score = 27.5 bits (58), Expect = 5.7
Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = +3
Query: 249 SVLQQLNAXAKSLQGALGDXNGKXKEALEQSRQNIERTAEELRKGH-PDVEKNATALREK 425
S L+++ + + LQ ++ + N K SR+ +ER EEL+KG D + N LR
Sbjct: 203 SELKKVESKTERLQVSVDELNAKPLV----SREELERVYEELKKGKVGDSDVNIDKLRAY 258
Query: 426 LQAAVQNTV 452
+ V+ +
Sbjct: 259 ARDIVEKEI 267
>At5g04990.1 68418.m00528 sad1/unc-84 protein-related contains weak
similarity to Sad1/unc-84 protein-like 1
(Swiss-Prot:O94901) [Homo sapiens]
Length = 471
Score = 27.1 bits (57), Expect = 7.5
Identities = 16/71 (22%), Positives = 40/71 (56%)
Frame = +3
Query: 246 ESVLQQLNAXAKSLQGALGDXNGKXKEALEQSRQNIERTAEELRKGHPDVEKNATALREK 425
+S L+++ + +SL+ ++ + N K ++ +ER EEL+KG+ D + ++
Sbjct: 202 QSELKKIESRTESLEKSVDEVNAKPWV----TKDELERIYEELKKGNVDDSAFSEISIDE 257
Query: 426 LQAAVQNTVQE 458
L+A ++ +++
Sbjct: 258 LRAYARDIMEK 268
>At2g41640.1 68415.m05145 expressed protein contains Pfam domain,
PF04577: Protein of unknown function (DUF563)
Length = 500
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/48 (27%), Positives = 22/48 (45%)
Frame = +3
Query: 138 RTPHQGSSIRTLEQQFNSLTKSKDAQDFSKXWKDGXESVLQQLNAXAK 281
RT SSI N+ TK + + +++ W+ +Q+LN K
Sbjct: 118 RTNSASSSIFLFTSSTNNNTKPEKIKPYTRKWETSVMDTVQELNLITK 165
>At2g06750.1 68415.m00753 hypothetical protein similar to At5g28270,
At2g12100, At2g05450, At1g45090, At2g16180
Length = 435
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -3
Query: 292 PWRLLAKALSCCSTDSXPSFQXLLKSCASF 203
PW+ LA AL + SF L CASF
Sbjct: 133 PWKKLALALIIIVVEGRTSFLLTLDRCASF 162
>At5g51370.1 68418.m06369 F-box family protein similar to unknown
protein (emb|CAB82288.1) ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 355
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = -3
Query: 265 SCCSTDSXPSFQXLLKSCASFDLVSELNCC 176
SC D+ P + LL+SC + + + CC
Sbjct: 286 SCRKIDASPGPEKLLRSCPAMESLQLKRCC 315
>At2g32240.1 68415.m03940 expressed protein contains Pfam profile:
PF04508 viral A-type inclusion protein repeat
Length = 775
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/58 (24%), Positives = 28/58 (48%)
Frame = +3
Query: 327 ALEQSRQNIERTAEELRKGHPDVEKNATALREKLQAAVQNTVQESHKLXXKVXSNVQE 500
ALE ++ E + D+ K T+ EKLQ+ + + +E++++ S +E
Sbjct: 476 ALEAEKEQTANELEASKTTIEDLTKQLTSEGEKLQSQISSHTEENNQVNAMFQSTKEE 533
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,411,721
Number of Sequences: 28952
Number of extensions: 143682
Number of successful extensions: 715
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 713
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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