Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31786
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 191 2e-49
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 191 3e-49
At5g34940.2 68418.m04121 glycosyl hydrolase family 79 N-terminal... 29 1.4
At5g34940.1 68418.m04120 glycosyl hydrolase family 79 N-terminal... 29 1.4
At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putat... 29 2.5
At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putat... 29 2.5
At1g56000.1 68414.m06425 amine oxidase-related contains Pfam pro... 27 5.7
At1g55980.1 68414.m06421 expressed protein 27 5.7
At5g57710.1 68418.m07214 heat shock protein-related contains sim... 27 7.5
At5g24040.1 68418.m02824 F-box family protein similar to unknown... 27 7.5
At3g57290.1 68416.m06377 eukaryotic translation initiation facto... 27 7.5
At1g52530.1 68414.m05930 expressed protein ; expression supporte... 27 7.5
At5g58640.1 68418.m07348 selenoprotein-related contains weak sim... 27 9.9
At2g26360.1 68415.m03164 mitochondrial substrate carrier family ... 27 9.9
At1g73340.1 68414.m08489 cytochrome P450 family protein similar ... 27 9.9
At1g28710.1 68414.m03536 expressed protein similar to GI:2827651... 27 9.9
At1g15260.1 68414.m01826 expressed protein EST gb|N65467 comes f... 27 9.9
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1025
Score = 191 bits (466), Expect = 2e-49
Identities = 91/166 (54%), Positives = 117/166 (70%), Gaps = 6/166 (3%)
Frame = +2
Query: 26 FIGEKEKALPLREILNRLEQAYCNNIGIEFMFINSLEQCNWIRQRMEPPNVTKMNADQKR 205
F+ E LR IL+RLEQAYC IG E+M I ++CNW+R ++E P + N++++
Sbjct: 181 FLSENRPVQTLRSILSRLEQAYCGTIGYEYMHIADRDKCNWLRDKIETPTPRQYNSERRM 240
Query: 206 LILARLTRSTGFENFLAKKWSSEKRFGLEGCEILIPAMKQVIDVSTKLGVESIIMGMPHR 385
+I RLT ST FENFLA KW++ KRFGLEG E LIP MK++ D S LGVE+I++GMPHR
Sbjct: 241 VIYDRLTWSTQFENFLATKWTTAKRFGLEGAESLIPGMKEMFDRSADLGVENIVIGMPHR 300
Query: 386 GRLNVLANVCRKPLHQLFTQFAGLEAEDD------GSGDVKYHLGT 505
GRLNVL NV RKPL Q+F++F+G D G+GDVKYHLGT
Sbjct: 301 GRLNVLGNVVRKPLRQIFSEFSGGTRPVDEVGLYTGTGDVKYHLGT 346
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1017
Score = 191 bits (465), Expect = 3e-49
Identities = 91/165 (55%), Positives = 118/165 (71%), Gaps = 5/165 (3%)
Frame = +2
Query: 26 FIGEKEKALPLREILNRLEQAYCNNIGIEFMFINSLEQCNWIRQRMEPPNVTKMNADQKR 205
F+ E LR IL RLEQAYC NIG E+M I ++CNW+R+++E P + N +++
Sbjct: 178 FMSENRPVQTLRSILTRLEQAYCGNIGFEYMHIADRDKCNWLREKIETPTPWRYNRERRE 237
Query: 206 LILARLTRSTGFENFLAKKWSSEKRFGLEGCEILIPAMKQVIDVSTKLGVESIIMGMPHR 385
+IL RL ST FENFLA KW++ KRFGLEG E LIP MK++ D + LGVESI++GM HR
Sbjct: 238 VILDRLAWSTQFENFLATKWTTAKRFGLEGGESLIPGMKEMFDRAADLGVESIVIGMSHR 297
Query: 386 GRLNVLANVCRKPLHQLFTQFA-GLEAEDD----GSGDVKYHLGT 505
GRLNVL NV RKPL Q+F++F+ G+ D+ G+GDVKYHLGT
Sbjct: 298 GRLNVLGNVVRKPLRQIFSEFSGGIRPVDEVGYTGTGDVKYHLGT 342
>At5g34940.2 68418.m04121 glycosyl hydrolase family 79 N-terminal
domain-containing protein similar to beta-glucuronidase
precursor [Scutellaria baicalensis] GI:8918740; contains
Pfam profile PF03662: Glycosyl hydrolase family 79,
N-terminal domain
Length = 536
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = -1
Query: 186 IFVTFGGSMRCRIQLHCSREFMNMNSIPMLLQYACSKRFRISLNGS 49
+F TF G+ + R HC+R+ + + M L + ++ LN S
Sbjct: 395 LFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNS 440
>At5g34940.1 68418.m04120 glycosyl hydrolase family 79 N-terminal
domain-containing protein similar to beta-glucuronidase
precursor [Scutellaria baicalensis] GI:8918740; contains
Pfam profile PF03662: Glycosyl hydrolase family 79,
N-terminal domain
Length = 401
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = -1
Query: 186 IFVTFGGSMRCRIQLHCSREFMNMNSIPMLLQYACSKRFRISLNGS 49
+F TF G+ + R HC+R+ + + M L + ++ LN S
Sbjct: 260 LFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNS 305
>At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profiles PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase, PF01593: amine oxidase, flavin-containing
Length = 867
Score = 28.7 bits (61), Expect = 2.5
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +3
Query: 363 SLWACPIEGASTYSRTCAVSLCTSCSH 443
S+W+CP +G ++S +S C C+H
Sbjct: 173 SIWSCPSDGVLSFSAYSVLSFC--CNH 197
>At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profile PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase
Length = 867
Score = 28.7 bits (61), Expect = 2.5
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +3
Query: 363 SLWACPIEGASTYSRTCAVSLCTSCSH 443
S+W+CP +G ++S +S C C+H
Sbjct: 173 SIWSCPSDGVLSFSAYSVLSFC--CNH 197
>At1g56000.1 68414.m06425 amine oxidase-related contains Pfam
profile PF01593: amine oxidase, flavin-containing
Length = 384
Score = 27.5 bits (58), Expect = 5.7
Identities = 8/32 (25%), Positives = 18/32 (56%)
Frame = +2
Query: 68 LNRLEQAYCNNIGIEFMFINSLEQCNWIRQRM 163
+N + +A CN G++ MF + + W+ + +
Sbjct: 136 MNSISKALCNESGVKSMFGTGIAKMEWLEEEI 167
>At1g55980.1 68414.m06421 expressed protein
Length = 464
Score = 27.5 bits (58), Expect = 5.7
Identities = 8/32 (25%), Positives = 18/32 (56%)
Frame = +2
Query: 68 LNRLEQAYCNNIGIEFMFINSLEQCNWIRQRM 163
+N + +A CN G++ MF + + W+ + +
Sbjct: 274 MNSISKALCNESGVKSMFGTGIAKMEWLEEEI 305
>At5g57710.1 68418.m07214 heat shock protein-related contains
similarity to 101 kDa heat shock protein; HSP101
[Triticum aestivum] gi|11561808|gb|AAC83689
Length = 990
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +2
Query: 8 KLPSTTFIGEKEKALPLREILNRLEQAYCNNIGIEFMFINSLEQ 139
K + +G+ E +REIL ++E N+ ++ + SLE+
Sbjct: 230 KKKNPVLVGDSEPGRVIREILKKIEVGEVGNLAVKNSKVVSLEE 273
>At5g24040.1 68418.m02824 F-box family protein similar to unknown
protein (gb|AAD22308.1) ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 373
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -1
Query: 264 HFLARKFSNPVDLVRRARMSLFWSAFIFVTFG--GSMRCRIQL 142
H ++ K NP DL+ + FW + + F S+RC + L
Sbjct: 13 HLISLKIDNPFDLIHFRSVCSFWRSSSLLKFRHMTSLRCPLPL 55
>At3g57290.1 68416.m06377 eukaryotic translation initiation factor
3E / eIF3e (TIF3E1) identical to eukaryotic initiation
factor 3E subunit [Arabidopsis thaliana]
gi|12407658|gb|AAG53613
Length = 441
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/58 (25%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Frame = -1
Query: 255 ARKFSNPVDLVRRARMSLFWSAFIFVTFGGSMRCRIQLHCSREFMN--MNSIPMLLQY 88
++ FS+P++ V+ + W +IF I L +++N S P LL+Y
Sbjct: 200 SKSFSSPLNQVQNRIWLMHWGLYIFFNHDNGRTQIIDLFNQDKYLNAIQTSAPHLLRY 257
>At1g52530.1 68414.m05930 expressed protein ; expression supported
by MPSS
Length = 344
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 324 CFIAGIKISHPSKPKRFSEDHFLARKFSNPVDLVRRARMSLF 199
C I+ ++ PS +RF E F A N V+L+ + + F
Sbjct: 7 CLISSTQLQFPSSSRRFREMKFKAFLTENGVNLLEKRFLPAF 48
>At5g58640.1 68418.m07348 selenoprotein-related contains weak
similarity to Selenoprotein W (Swiss-Prot:P49904)
[Rattus norvegicus]
Length = 228
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/39 (28%), Positives = 18/39 (46%)
Frame = +3
Query: 360 PSLWACPIEGASTYSRTCAVSLCTSCSHSSPVSKLRMMV 476
PS + GA Y T ++ C SCS+ ++ M+
Sbjct: 59 PSQKTSGLGGAIGYGNTVEINFCVSCSYKGTAVTMKKML 97
>At2g26360.1 68415.m03164 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 303
Score = 26.6 bits (56), Expect = 9.9
Identities = 20/63 (31%), Positives = 28/63 (44%)
Frame = -3
Query: 508 VRAQVIFHVAGTIILSFETGELCEQLVQRLTAHVREYVEAPSMGHAHNDGFDSEFRGNVD 329
V+ Q I GT+ L CE L QRL A+ + + ++ H +G FRG
Sbjct: 117 VKVQSIASFIGTV-LGTTLRIPCEVLKQRLQANQFDNIVEATVSTWHQEGLKGLFRGTGV 175
Query: 328 DLL 320
LL
Sbjct: 176 TLL 178
>At1g73340.1 68414.m08489 cytochrome P450 family protein similar to
Cytochrome P450 90A1 (SP:Q42569) [Arabidopsis thaliana];
contains Pfam profile: PF00067 cytochrome P450
Length = 512
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = -3
Query: 199 LVSVHLCYVRWLHALSDPVALFQGIYEHELNTDVVAVRLLQTIQNFSQRE 50
+++VH R LH+++ + + H L +V+ V +LQT+ NF E
Sbjct: 154 VITVHGDQQRRLHSIASSMMRHDQLKTHFL--EVIPVVMLQTLSNFKDGE 201
>At1g28710.1 68414.m03536 expressed protein similar to GI:2827651,
GI:7527728, GI:4406788, GI:6063544, GI:10764853 from
[Arabidopsis thaliana]
Length = 340
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/53 (26%), Positives = 29/53 (54%)
Frame = +2
Query: 77 LEQAYCNNIGIEFMFINSLEQCNWIRQRMEPPNVTKMNADQKRLILARLTRST 235
L+ + N +G+ F+N+ E + + + VT ++A+ R ILA+++ T
Sbjct: 251 LDSGFFNQLGLNVGFLNTTEFSGFCQDSHDMGVVTTVHANCCRHILAKISDLT 303
>At1g15260.1 68414.m01826 expressed protein EST gb|N65467 comes from
this gene
Length = 178
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/23 (43%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = -1
Query: 138 CSREFMNM-NSIPMLLQYACSKR 73
CS ++ N+ N P+L Y+CS+R
Sbjct: 28 CSSDYKNLPNDTPLLSPYSCSRR 50
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,962,060
Number of Sequences: 28952
Number of extensions: 250719
Number of successful extensions: 744
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 732
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 741
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -