BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31783 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g24550.1 68416.m03083 protein kinase family protein contains ... 31 0.46 At1g13830.1 68414.m01623 beta-1,3-glucanase-related similar to b... 31 0.61 At2g40070.1 68415.m04923 expressed protein 30 0.80 At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 30 1.1 At3g22070.1 68416.m02785 proline-rich family protein contains pr... 28 3.2 At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 28 3.2 At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin fa... 28 4.3 At3g28790.1 68416.m03593 expressed protein 27 5.7 At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family pro... 27 5.7 At1g06260.1 68414.m00662 cysteine proteinase, putative contains ... 27 5.7 At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 27 7.5 At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138... 27 9.9 At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 27 9.9 At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 27 9.9 At1g26150.1 68414.m03192 protein kinase family protein similar t... 27 9.9 >At3g24550.1 68416.m03083 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain Length = 652 Score = 31.1 bits (67), Expect = 0.46 Identities = 26/76 (34%), Positives = 32/76 (42%), Gaps = 6/76 (7%) Frame = +3 Query: 24 PSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTP---WPSTPHRXMPIPEWVRNPAILP-- 188 PSS P L P S T P Q + SA +TP P+TP P + P P Sbjct: 56 PSSPLPPSLPPPSPPGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGPPNTPSG 115 Query: 189 -IARAASNSVPKYPAD 233 R SN+ P P+D Sbjct: 116 STPRTPSNTKPSPPSD 131 >At1g13830.1 68414.m01623 beta-1,3-glucanase-related similar to beta-1,3-glucanase-like protein (GI:14279169) [Olea europaea] similar to Glucan endo-1,3-beta-glucosidase precursor (EC 3.2.1.39) ((1-3)-beta- glucan endohydrolase) ((1-3)-beta-glucanase) (Beta-1,3- endoglucanase) (Swiss-Prot:P52409) [Triticum aestivum] Length = 197 Score = 30.7 bits (66), Expect = 0.61 Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Frame = +3 Query: 54 PASTQ-PDSTQPFAQTTRSAMLTPWPSTPHRXMPIP 158 P+ST+ P ST P TT + TP+P TP P P Sbjct: 110 PSSTRSPPSTTPPTGTTPTNGTTPFPGTPFPGTPFP 145 >At2g40070.1 68415.m04923 expressed protein Length = 607 Score = 30.3 bits (65), Expect = 0.80 Identities = 23/78 (29%), Positives = 36/78 (46%), Gaps = 1/78 (1%) Frame = +3 Query: 9 RXSCSPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPW-PSTPHRXMPIPEWVRNPAIL 185 R + PS R P + +P ++ A TT + ++ PS+P P+P +NPA+ Sbjct: 301 RPTLPPSKTISRSSTP-TRRPIASASAATTTANPTISQIKPSSPAPAKPMPTPSKNPALS 359 Query: 186 PIARAASNSVPKYPADFP 239 A S P P+D P Sbjct: 360 RAASPTVRSRPWKPSDMP 377 >At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catalytic subunit, putative similar to SP|O48653 DNA polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza sativa}; contains Pfam profiles: PF03175 DNA polymerase type B, organellar and viral, PF00136 DNA polymerase family B, PF03104 DNA polymerase family B, exonuclease domain Length = 1492 Score = 29.9 bits (64), Expect = 1.1 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = +2 Query: 86 ICPNYPFCDADALAKYT 136 +CPNYP C+ L KYT Sbjct: 1407 VCPNYPNCNGTLLRKYT 1423 >At3g22070.1 68416.m02785 proline-rich family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 178 Score = 28.3 bits (60), Expect = 3.2 Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 1/70 (1%) Frame = +3 Query: 57 ASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPADF 236 +ST P S+ + ++ S +TP+P+ P P + + P ++S S+ P Sbjct: 45 SSTDPSSSSSSSSSSTSPFITPFPNPNPNPNPNPPVLGSSPPSPTDSSSSTSISPNP--- 101 Query: 237 PAALC-PNYP 263 PA + PN P Sbjct: 102 PAPIVNPNPP 111 >At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-containing protein low similarity to AHM1 [Triticum aestivum] GI:6691467; contains Pfam profile PF00226: DnaJ domain Length = 797 Score = 28.3 bits (60), Expect = 3.2 Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 4/75 (5%) Frame = +3 Query: 51 WPASTQPDSTQPFAQTTRSAMLTPWP----STPHRXMPIPEWVRNPAILPIARAASNSVP 218 +P S P +++PF + S P+P S + + + + + LP++++ NS P Sbjct: 393 FPLSQPPSNSKPFPMSQSSQNSKPFPVSQSSQKSKPLLVSQSSQRSKPLPVSQSLQNSNP 452 Query: 219 KYPADFPAALCPNYP 263 +P P++ +P Sbjct: 453 -FPVSQPSSNSKPFP 466 Score = 28.3 bits (60), Expect = 3.2 Identities = 17/75 (22%), Positives = 39/75 (52%), Gaps = 4/75 (5%) Frame = +3 Query: 51 WPASTQPDSTQPF--AQTTRSA--MLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVP 218 +P S +++PF +Q+++ + +L S + +P+ + ++N P+++ +SNS P Sbjct: 405 FPMSQSSQNSKPFPVSQSSQKSKPLLVSQSSQRSKPLPVSQSLQNSNPFPVSQPSSNSKP 464 Query: 219 KYPADFPAALCPNYP 263 +P P +P Sbjct: 465 -FPVSQPQPASNPFP 478 >At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin family protein contains Pfam profile PF01190: Pollen proteins Ole e I family Length = 401 Score = 27.9 bits (59), Expect = 4.3 Identities = 22/69 (31%), Positives = 27/69 (39%), Gaps = 2/69 (2%) Frame = +3 Query: 54 PASTQPDSTQPFAQTTRSAMLTPWPSTPHRX-MP-IPEWVRNPAILPIARAASNSVPKYP 227 P S P P T + L P P TP +P IP P + P+ S+P P Sbjct: 295 PPSLPPIPLIPTPPTLPTIPLLPTPPTPTLPPIPTIPTLPPLPVLPPVPIVNPPSLPPPP 354 Query: 228 ADFPAALCP 254 FP L P Sbjct: 355 PSFPVPLPP 363 >At3g28790.1 68416.m03593 expressed protein Length = 608 Score = 27.5 bits (58), Expect = 5.7 Identities = 17/57 (29%), Positives = 21/57 (36%) Frame = +3 Query: 60 STQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPA 230 +T D+T + + S TP PSTP P P P S P PA Sbjct: 261 NTYKDTTGSSSGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPSTPAPSTPA 317 >At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family protein low similarity to ubiquitin-conjugating BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831, ubiquitin-conjugating enzyme [Mus musculus] GI:3319990; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 907 Score = 27.5 bits (58), Expect = 5.7 Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 4/34 (11%) Frame = +2 Query: 92 PNYPFCDADALAKYTPQG----NADTRMGAQPSH 181 P++ FC +D + K P+G NADT + + H Sbjct: 397 PDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKH 430 >At1g06260.1 68414.m00662 cysteine proteinase, putative contains similarity to thiol-protease, pre-pro-TPE4A protein GI:3688528 [Pisum sativum] Length = 343 Score = 27.5 bits (58), Expect = 5.7 Identities = 18/73 (24%), Positives = 30/73 (41%) Frame = -3 Query: 244 AAGKSAGYLGTEFDAARAIGRMAGLRTHSGIGIXLWGVLGQGVSIAERVVWANGWVESGW 65 + G AG + ++ G + G+ + +GV G + W GW E G+ Sbjct: 259 SVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEEGY 318 Query: 64 VLAGQSYRGQSED 26 + + RG SED Sbjct: 319 I---RMERGVSED 328 >At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor family protein low similarity to extensin [Volvox carteri] GI:21992 Length = 312 Score = 27.1 bits (57), Expect = 7.5 Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 1/82 (1%) Frame = +3 Query: 15 SCSPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIA 194 S SPSS P L P+S P ++ S L+P P + P + + P++ Sbjct: 52 SLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSPPSSSPSSAPPSSLSPSSPPPLS 111 Query: 195 RAASNSVPKYPADFP-AALCPN 257 + S+ P P+ P ++L P+ Sbjct: 112 LSPSSPPPPPPSSSPLSSLSPS 133 Score = 26.6 bits (56), Expect = 9.9 Identities = 25/83 (30%), Positives = 33/83 (39%) Frame = +3 Query: 15 SCSPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIA 194 S SPSS P L P+S P S P + +P S P P P+ P + Sbjct: 42 SSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSP-----PSSSPSS 96 Query: 195 RAASNSVPKYPADFPAALCPNYP 263 S+ P P P +L P+ P Sbjct: 97 APPSSLSPSSPP--PLSLSPSSP 117 >At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'PsRT17-1 like protein' based on similarity to PsRT17-1 (GP:1778376) [Pisum sativum] which was based upon similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 519 Score = 26.6 bits (56), Expect = 9.9 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -3 Query: 142 LWGVLGQGVSIAERVVWANGWVESGWVLAGQSYRGQS 32 L G L + +SI ++V + + GW +SYRG S Sbjct: 60 LQGALDRQISIRQQVELWSPLADQGWKPCTESYRGAS 96 >At3g19020.1 68416.m02415 leucine-rich repeat family protein / extensin family protein similar to extensin-like protein [Lycopersicon esculentum] gi|5917664|gb|AAD55979; contains leucine-rich repeats, Pfam:PF00560; contains proline rich extensin domains, INTERPRO:IPR002965 Length = 956 Score = 26.6 bits (56), Expect = 9.9 Identities = 21/82 (25%), Positives = 28/82 (34%) Frame = +3 Query: 21 SPSSLWPR*LWPASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARA 200 SP P+ P + QP Q T +P P P + P PE P + Sbjct: 533 SPKPQPPKQETPKPEESPKPQPPKQETPKPEESPKPQPPKQETPKPEESPKPQPPKQEQP 592 Query: 201 ASNSVPKYPADFPAALCPNYPY 266 PK + + PN PY Sbjct: 593 PKTEAPKMGSPPLESPVPNDPY 614 >At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 665 Score = 26.6 bits (56), Expect = 9.9 Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 4/69 (5%) Frame = +3 Query: 54 PAST-QPDSTQPFAQTTRSAMLTP--WPSTPHRXM-PIPEWVRNPAILPIARAASNSVPK 221 P+S +P+ P+ A TP P P R P P + +PA P + V K Sbjct: 338 PSSVFRPERVSPWELEPLVANSTPSSQPQPPQRNKRPRPPGLPSPATGPSGPVTPDGVWK 397 Query: 222 YPADFPAAL 248 PAD P+++ Sbjct: 398 SPADTPSSV 406 >At1g26150.1 68414.m03192 protein kinase family protein similar to Pto kinase interactor 1 GI:3668069 from [Lycopersicon esculentum] Length = 760 Score = 26.6 bits (56), Expect = 9.9 Identities = 19/70 (27%), Positives = 28/70 (40%) Frame = +3 Query: 54 PASTQPDSTQPFAQTTRSAMLTPWPSTPHRXMPIPEWVRNPAILPIARAASNSVPKYPAD 233 P T+ T P + P P +P +P P+ NP P S+S P++ Sbjct: 127 PPPTEAPPTTPITSPSPPTNPPPPPESPPS-LPAPDPPSNPLPPPKLVPPSHSPPRHLPS 185 Query: 234 FPAALCPNYP 263 PA+ P P Sbjct: 186 PPASEIPPPP 195 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,661,732 Number of Sequences: 28952 Number of extensions: 280101 Number of successful extensions: 704 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 675 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 702 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -