BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31773 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 353 1e-98 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 205 3e-54 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 188 3e-49 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 145 3e-36 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 106 2e-24 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 104 9e-24 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 93 3e-20 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 53 2e-08 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 38 0.001 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.10 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.18 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.9 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 3.8 SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 6.7 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 6.7 SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.9 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 8.9 SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.9 SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 353 bits (867), Expect = 1e-98 Identities = 159/172 (92%), Positives = 168/172 (97%) Frame = +1 Query: 1 VAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGI 180 +AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSKRGI Sbjct: 5 IAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGI 64 Query: 181 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 360 LTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+F Sbjct: 65 LTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIF 124 Query: 361 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 516 ETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+ Sbjct: 125 ETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIM 176 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 205 bits (500), Expect = 3e-54 Identities = 92/169 (54%), Positives = 126/169 (74%), Gaps = 1/169 (0%) Frame = +1 Query: 10 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 189 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71 Query: 190 KYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 366 + PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + +ET Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131 Query: 367 FNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 513 N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI Sbjct: 132 LNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAI 180 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 188 bits (459), Expect = 3e-49 Identities = 87/172 (50%), Positives = 123/172 (71%), Gaps = 2/172 (1%) Frame = +1 Query: 4 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 177 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 178 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 357 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 358 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 513 FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLV 175 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 145 bits (352), Expect = 3e-36 Identities = 82/200 (41%), Positives = 113/200 (56%), Gaps = 32/200 (16%) Frame = +1 Query: 10 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 138 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 139 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 297 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 298 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 453 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186 Query: 454 GDGVSHTVPIYEGYALPHAI 513 GDGV+H +P+ EGY + +I Sbjct: 187 GDGVTHIIPVAEGYVIGSSI 206 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 106 bits (254), Expect = 2e-24 Identities = 55/164 (33%), Positives = 97/164 (59%), Gaps = 3/164 (1%) Frame = +1 Query: 10 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 189 LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++ Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGNDTLIEVGSRSI 82 Query: 190 -KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 366 + P E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE Sbjct: 83 ARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFEL 141 Query: 367 FNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEG 492 +N+P++ I + S Y + + ++GIVL+ G+ SH +P+ G Sbjct: 142 YNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 104 bits (249), Expect = 9e-24 Identities = 58/167 (34%), Positives = 89/167 (53%) Frame = +1 Query: 1 VAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGI 180 V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S G Sbjct: 11 VSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNPG- 62 Query: 181 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 360 + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 361 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 501 E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 92.7 bits (220), Expect = 3e-20 Identities = 49/173 (28%), Positives = 92/173 (53%), Gaps = 2/173 (1%) Frame = +1 Query: 1 VAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGI 180 + +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q I Sbjct: 10 IPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHAPI 63 Query: 181 --LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 354 + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ + Sbjct: 64 PGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEA 123 Query: 355 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 513 FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I Sbjct: 124 AFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGI 176 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 53.2 bits (122), Expect = 2e-08 Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 4/103 (3%) Frame = +1 Query: 196 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 363 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 364 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 492 P +A + ++ LYA G G+V+D G + PI +G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 37.5 bits (83), Expect = 0.001 Identities = 42/174 (24%), Positives = 70/174 (40%), Gaps = 6/174 (3%) Frame = +1 Query: 10 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 189 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 190 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 357 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 358 FETFNSPAMYVAIQAVLSLYASGRTTGIVLD--SGDGVSHTVPIYEGYALPHAI 513 FE F A A L + G T D +G + I GY+ H I Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHII 171 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.10 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +1 Query: 55 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 198 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.18 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 256 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 399 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 2.9 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 298 LLTEAPLNPKANREKMTQIMFETFN 372 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 25.8 bits (54), Expect = 3.8 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = -2 Query: 98 RPTMDGKTARGASSPAKPALHMPEPLS 18 RP+ DG+ A G A P++ P P+S Sbjct: 950 RPSNDGRKASGPRPAAPPSIPPPLPVS 976 >SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Schizosaccharomyces pombe|chr 3|||Manual Length = 791 Score = 25.0 bits (52), Expect = 6.7 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = -2 Query: 251 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 105 WC+ S + SQ+ + +G F PL L WA + + P ITPWW Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +1 Query: 115 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 201 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 >SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 24.6 bits (51), Expect = 8.9 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%) Frame = +1 Query: 22 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 174 N +GM + R F S VG + VG Q+DSY+ D++ S+R Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 24.6 bits (51), Expect = 8.9 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = -1 Query: 219 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 115 GDD+VLD V +D L L AH D H Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710 >SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 24.6 bits (51), Expect = 8.9 Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%) Frame = +2 Query: 467 PTPYPSTKVTLC-PTP 511 P P P TKV +C PTP Sbjct: 256 PAPSPETKVLICGPTP 271 >SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 24.6 bits (51), Expect = 8.9 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = +1 Query: 451 SGDGVSHTVPIYEGYALP 504 +G G+ H + + +GYA+P Sbjct: 157 AGRGIGHQIMVEQGYAMP 174 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,968,074 Number of Sequences: 5004 Number of extensions: 39163 Number of successful extensions: 163 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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