BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31757 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 25 6.7 >SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.0 bits (52), Expect = 6.7 Identities = 42/183 (22%), Positives = 76/183 (41%), Gaps = 14/183 (7%) Frame = +3 Query: 3 SLKVYITYKICMFTCECFLNCKSYLLTKPSTICNIFTKFLIVVFILINKIFIVYSKRYI* 182 +LK YI + IC+F L Y S + F I++ + I ++V +++ Sbjct: 159 NLKNYIAFPICLFVINHILILLGYFQCSYSVF--YASVFYILLGVFIRVFYLVNKEKF-- 214 Query: 183 LRRNKKSI*LF*ISLRNYCLIK--LVLYG*IIIGCC-FTFYSFNDIYIIS-SGKT----- 335 KK + S+ CLI+ ++ G I + FT F I+ I+ G Sbjct: 215 ---EKKELAFLFSSIVVACLIQFNVLPLGTINLSVTRFTILCFMQIFCINWDGIARIQFY 271 Query: 336 --NFESKLVSFSINELINK*SDKGCGILVSVIILRKFYY---NSNIYVSQVSLMNKKICN 500 F+ L+ I+ +INK + + ++S + F + S+I + N +I Sbjct: 272 LGKFDISLIMALISAIINKTASQNSIKIISCLYQVGFCFFKIGSSITANLKLPDNSRIYR 331 Query: 501 IYN 509 +YN Sbjct: 332 LYN 334 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,747,192 Number of Sequences: 5004 Number of extensions: 29368 Number of successful extensions: 50 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 49 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 50 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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