Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31733
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 30 1.1
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 30 1.1
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 29 1.4
At1g05790.1 68414.m00605 lipase class 3 family protein contains ... 29 1.9
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 29 2.5
At1g72110.1 68414.m08335 expressed protein 29 2.5
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 3.2
At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to a... 27 5.7
At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to e... 27 5.7
At3g13780.1 68416.m01739 expressed protein 27 5.7
At1g26930.1 68414.m03283 kelch repeat-containing F-box family pr... 27 5.7
At1g09070.1 68414.m01012 C2 domain-containing protein / src2-lik... 27 5.7
At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 27 7.5
At5g56970.1 68418.m07111 FAD-binding domain-containing protein /... 27 9.9
At3g53080.1 68416.m05850 galactose-binding lectin family protein... 27 9.9
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +1
Query: 220 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 390
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +1
Query: 220 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 390
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 29.5 bits (63), Expect = 1.4
Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +1
Query: 304 TNNGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGXA 477
T+ G + G G P P + S +S TSP P S +Y Y PT +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614
Query: 478 I-PTSP 492
PTSP
Sbjct: 1615 YSPTSP 1620
Score = 29.1 bits (62), Expect = 1.9
Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 1/72 (1%)
Frame = +1
Query: 280 GSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 459
G + ++ G + G G P P +S TSP P S Y GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587
Query: 460 QPTGXAI-PTSP 492
PT A PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S Y GY PT + PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/43 (39%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSPPVPEQIAR 516
S+S TSP P S Y Y PT + PTSP Q A+
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAK 1733
Score = 27.9 bits (59), Expect = 4.3
Identities = 27/97 (27%), Positives = 39/97 (40%), Gaps = 5/97 (5%)
Frame = +1
Query: 217 PKVSEGYGAETVKFGNEINPDGSYTY--FYETNNGIAAQEQGVPRNLGGNPPAVPVVA-- 384
P S GY + + + +P S T + T+ G + + G P P +
Sbjct: 1539 PSSSPGYSPSSPGY-SPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPT 1597
Query: 385 QGSFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1598 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1634
Score = 27.5 bits (58), Expect = 5.7
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1607 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1641
Score = 27.5 bits (58), Expect = 5.7
Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S Y Y PT A PTSP
Sbjct: 1628 SYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSP 1662
Score = 27.5 bits (58), Expect = 5.7
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1663 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1697
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/35 (42%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +1
Query: 391 SFSWTSPEGVPISVNYVADENGYQPTGXAI-PTSP 492
S+S TSP P S +Y Y PT PTSP
Sbjct: 1677 SYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSP 1711
>At1g05790.1 68414.m00605 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 687
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -1
Query: 486 SGDSXTSGLVSVLVGDVIDADGNTFR 409
SG+S +SG +S L+GD + DG + R
Sbjct: 398 SGESESSGFLSSLIGDGCECDGYSIR 423
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 28.7 bits (61), Expect = 2.5
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -3
Query: 421 EHLQEMSRRMSPVQRREQQGGFHQGYVELPVLGQ 320
E+ +E S+R+ P R+Q+ QG+++ P GQ
Sbjct: 27 ENREEHSKRLRPTSFRDQKVTIPQGWLDCPRFGQ 60
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +1
Query: 235 YGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNL 351
+G+ T+ F N + PD ++F IAA GVP+ L
Sbjct: 388 FGSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +2
Query: 344 VTLVETPLLFPSLHRAHSPGHLLKVFPSASITSPTRT 454
++LV TPLLF + A + G LL+ FPS + +SP +
Sbjct: 518 LSLVTTPLLFKLIPSAMNLGVLLRWFPSEN-SSPNES 553
>At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to
alpha-expansin 4 precursor GI:16923355 from [Cucumis
sativus]; alpha-expansin gene family, PMID:11641069
Length = 260
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -3
Query: 190 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 83
++GW + W + T G+ TG G S R+ G+
Sbjct: 201 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 236
>At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to
expansin2 GI:4884433 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 259
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -3
Query: 190 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 83
++GW + W + T G+ TG G S R+ G+
Sbjct: 200 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 235
>At3g13780.1 68416.m01739 expressed protein
Length = 309
Score = 27.5 bits (58), Expect = 5.7
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -1
Query: 210 SDGGNRGDRDGQKRSRSGHGNER 142
++G + GD DG K+ R G G +R
Sbjct: 177 TNGNSDGDEDGDKKGRKGLGKKR 199
>At1g26930.1 68414.m03283 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 421
Score = 27.5 bits (58), Expect = 5.7
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +1
Query: 208 RVVPKVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGN 360
R K S G + + FG E++ Y Y TN+ A+ +PR L G+
Sbjct: 157 RYADKESLAVGTDLLVFGWEVSSYVIYRYSLLTNSWSTAKSMNMPRCLFGS 207
>At1g09070.1 68414.m01012 C2 domain-containing protein / src2-like
protein, putative similar to cold-regulated gene SRC2
[Glycine max] GI:2055230; contains Pfam profile PF00168:
C2 domain; identical to cDNA src2-like protein
GI:3426059
Length = 324
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/55 (30%), Positives = 21/55 (38%), Gaps = 6/55 (10%)
Frame = +1
Query: 325 QEQGVPRNLGGNPPAVPVVAQGSFSWTSPE------GVPISVNYVADENGYQPTG 471
Q+ G P GG PP QG + P+ G P Y + GY P G
Sbjct: 204 QQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPGYPPQGPYGYPQQGYPPQG 258
>At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 889
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +3
Query: 78 RPSPV*K*ILQEEPIPVQHIPAVRSRDHSYSVSAHPDLLYCPRQSS 215
RPSP+ ++EE P+ ++P +RS + S S LY P SS
Sbjct: 67 RPSPMRGLPMEEETKPIVYLPRIRSEVFASSPSLLN--LYSPSSSS 110
>At5g56970.1 68418.m07111 FAD-binding domain-containing protein /
cytokinin oxidase family protein similar to cytokinin
oxidase from Zea mays [gi:3882018] [gi:3341978]
Length = 523
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 401 GHLLKVFPSASITSPTRTDTSPLVMLS 481
GH+ K+FPSA + + D + L+ LS
Sbjct: 63 GHVTKIFPSAVLIPSSVEDITDLIKLS 89
>At3g53080.1 68416.m05850 galactose-binding lectin family protein
contains Pfam domain PF02140: Galactose binding lectin
domain
Length = 155
Score = 26.6 bits (56), Expect = 9.9
Identities = 17/65 (26%), Positives = 27/65 (41%)
Frame = +1
Query: 232 GYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSP 411
GY + F + NP G+ +F N G A + V +N G +V F +
Sbjct: 81 GYVITKINFADYGNPTGTCGHFRRGNCGARATMRIVKKNCLGKEKCHLLVTDEMFGPSKC 140
Query: 412 EGVPI 426
+G P+
Sbjct: 141 KGAPM 145
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.136 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,371,858
Number of Sequences: 28952
Number of extensions: 252028
Number of successful extensions: 771
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 718
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 760
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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