Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31727
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 82 4e-17
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 81 1e-16
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 55 5e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 41 1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 40 2e-04
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.55
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 1.7
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.7
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 26 3.8
SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase Ubp9|Schizosac... 25 5.1
SPBC18E5.07 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 5.1
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 6.7
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 8.9
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 82.2 bits (194), Expect = 4e-17
Identities = 45/122 (36%), Positives = 66/122 (54%), Gaps = 2/122 (1%)
Frame = +1
Query: 157 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 336
++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87
Query: 337 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 510
M + PIN LT + + S +D ++ AF TFD + GKI +R AL +
Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146
Query: 511 GD 516
GD
Sbjct: 147 GD 148
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 80.6 bits (190), Expect = 1e-16
Identities = 44/111 (39%), Positives = 66/111 (59%), Gaps = 6/111 (5%)
Frame = +1
Query: 202 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 369
+ +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 370 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGD 516
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G+
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116
Score = 37.1 bits (82), Expect = 0.002
Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%)
Frame = +1
Query: 220 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLT 387
E +EAF++ D D +G I +L SLG S++E+ +M+ EA G IN+ +
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145
Query: 388 LFANR 402
+ +++
Sbjct: 146 VISSK 150
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.2 bits (127), Expect = 5e-09
Identities = 32/107 (29%), Positives = 59/107 (55%), Gaps = 2/107 (1%)
Frame = +1
Query: 202 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 381
S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 382 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGD 516
++ +N++ E++ I AF+ FD++ G I++ + + T G+
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGE 109
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 40.7 bits (91), Expect = 1e-04
Identities = 18/42 (42%), Positives = 26/42 (61%)
Frame = +1
Query: 220 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 345
EF + FQ+ D D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 39.9 bits (89), Expect = 2e-04
Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 6/103 (5%)
Frame = +1
Query: 202 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 369
+++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 370 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLR 492
+ + ++ D + + AF+ F DE GKI LR
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLR 133
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.55
Identities = 21/59 (35%), Positives = 28/59 (47%)
Frame = -2
Query: 488 SLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDAS 312
S S S SSS ++ +TSSSS L++ S +S ISSSS S +S
Sbjct: 224 SSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSS 282
Score = 26.2 bits (55), Expect = 2.9
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = -2
Query: 488 SLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDAS 312
S S S SSS ++I++SSSS ++ + S +SPT +SS+ S +S
Sbjct: 256 SSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISS---SSSSSSSPTSTSSTISSSS 311
Score = 25.8 bits (54), Expect = 3.8
Identities = 22/60 (36%), Positives = 27/60 (45%), Gaps = 1/60 (1%)
Frame = -2
Query: 488 SLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGP-EASPTISSSSFSDAS 312
S S S SSS + ++SSSS P + S P S TISSSS S +S
Sbjct: 257 SSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSSPTSTSSTISSSSSSSSS 316
Score = 24.6 bits (51), Expect = 8.9
Identities = 21/64 (32%), Positives = 29/64 (45%)
Frame = -2
Query: 488 SLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASL 309
S+S S SSS + +SSSS P ++ S K +S SSSS S ++L
Sbjct: 325 SMSSSSSFSSSPTSSSSTISSSSSSPSSSSFSSTTSS-SKSSSSFSSTVSSSSSTSSSTL 383
Query: 308 PRES 297
S
Sbjct: 384 TSSS 387
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 27.1 bits (57), Expect = 1.7
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = -2
Query: 485 LSESIF--PSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDAS 312
LS S F P+SS + +L+++SS S ASPT SSSS + +S
Sbjct: 66 LSSSSFTSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTSSSSSHALSS 125
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.1 bits (57), Expect = 1.7
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 220 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 345
E +EAF L D G I DLR + LG ++++L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 317
Score = 25.8 bits (54), Expect = 3.8
Identities = 20/65 (30%), Positives = 29/65 (44%)
Frame = -2
Query: 491 RSLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDAS 312
+S S S SSS ++SSSS ++ KS ++S SSSSF ++
Sbjct: 171 KSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSSSSSKSSASPSSSKSSSKFSSSSFITST 230
Query: 311 LPRES 297
P S
Sbjct: 231 TPASS 235
>SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase
Ubp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 585
Score = 25.4 bits (53), Expect = 5.1
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -2
Query: 500 RACRSLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS 378
+A R S S FPS ++ +S S+ P D L + KS
Sbjct: 521 KAVRRESRSFFPSLTRKRSKFFGSSQSNSPKDSPLRDTHKS 561
>SPBC18E5.07 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 615
Score = 25.4 bits (53), Expect = 5.1
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = -2
Query: 491 RSLSESIFPS-SSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGPEASP 345
RS + PS SS + ++SSSSEP ++ N VK +++ E P
Sbjct: 348 RSKKKHTGPSLSSASQPSAASSSSSSEPSNLDKINDVKKNIEVSANEPQP 397
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 211 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 324
+ A+FK+ F DKD + + +L A + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 24.6 bits (51), Expect = 8.9
Identities = 15/53 (28%), Positives = 25/53 (47%)
Frame = -2
Query: 515 SPQVMRACRSLSESIFPSSSKVLKALITTSSSSEPPDMRLANRVKS*VKLMGP 357
+P V+ + S P+S KA +TTS+ E P +++A + L P
Sbjct: 467 TPNVLSSLSSNLGKTNPTSVYQSKANVTTSADVEKPQVKVATSSRVDYDLKSP 519
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.133 0.373
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,607,859
Number of Sequences: 5004
Number of extensions: 25137
Number of successful extensions: 144
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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