BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31710 (435 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 3.8 SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 25 3.8 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 5.0 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 25.4 bits (53), Expect = 3.8 Identities = 10/29 (34%), Positives = 15/29 (51%) Frame = +3 Query: 141 YTCEQQASRTPTSVLCKVNEVSPRNSSKE 227 Y C+Q+ TSV+C + S +KE Sbjct: 1301 YACDQRGENCETSVVCSLRLTSAAAHAKE 1329 >SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 25.4 bits (53), Expect = 3.8 Identities = 15/57 (26%), Positives = 25/57 (43%) Frame = +3 Query: 198 EVSPRNSSKEARPGGGKT*S*TSNKLEQANKDLEEKEKQLTATVSRGRCPQQESAAD 368 +VSP+ S KEA+ S S K ++ +K EE + + S S ++ Sbjct: 48 DVSPKKSKKEAKRASSPEPSKKSVKKQKKSKKKEESSSESESESSSSESESSSSESE 104 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.0 bits (52), Expect = 5.0 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = +1 Query: 334 EVXALNRKVQQIEEDL*KSEE 396 +V +L + +Q ++EDL KSEE Sbjct: 683 DVDSLEKNIQTLKEDLRKSEE 703 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,416,292 Number of Sequences: 5004 Number of extensions: 23678 Number of successful extensions: 56 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 56 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 56 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 156095170 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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