BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31703 (400 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.35 SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 27 1.4 SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 4.3 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 5.7 SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 25 5.7 SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb... 25 5.7 SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 24 10.0 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 24 10.0 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 24 10.0 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.7 bits (61), Expect = 0.35 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -1 Query: 250 PPMAVPIPPNEVAMPSRPFSILSSSIPWSA 161 PPM+VP PP+ MP+ P S +P S+ Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739 >SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 26.6 bits (56), Expect = 1.4 Identities = 21/74 (28%), Positives = 31/74 (41%) Frame = -1 Query: 229 PPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPGVVVSKSRSH 50 P + V + S LSSS+ S + PA S K E+P P P V S SR Sbjct: 227 PGSFVYEDAEKLSQLSSSLISSFSEQPKEHSPATS--KHEPEETPASPTPSVSASTSRER 284 Query: 49 GVQIPESMVSQXRS 8 + S ++ ++ Sbjct: 285 STSVAPSFITSDQA 298 >SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 700 Score = 25.0 bits (52), Expect = 4.3 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -1 Query: 256 RAPPMAVPIPPNEVAMPSRPFS 191 R P M +P PP++ +PS P S Sbjct: 24 RQPSMNLPTPPHDDGLPSIPRS 45 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 24.6 bits (51), Expect = 5.7 Identities = 14/52 (26%), Positives = 27/52 (51%) Frame = -1 Query: 247 PMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPK 92 P + P PP + + S+P S SS++P K F+ + S ++++ P+ Sbjct: 193 PYSSPFPPPRIVLGSKPVS--SSTLPSKPKSFQVFSDASSSRDSQNASDLPQ 242 >SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1191 Score = 24.6 bits (51), Expect = 5.7 Identities = 25/76 (32%), Positives = 34/76 (44%), Gaps = 1/76 (1%) Frame = -1 Query: 253 APPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPGV 74 AP + + P + +P RP LS E+S PAL+ LSS SP P P Sbjct: 347 APASEMSLIPEKPRLPPRPSHTLS---------ELS--SPALTSENLSSKPSPLFPPPPP 395 Query: 73 -VVSKSRSHGVQIPES 29 V S + + V +P S Sbjct: 396 RVKSLATNKPVSMPVS 411 >SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 386 Score = 24.6 bits (51), Expect = 5.7 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = -1 Query: 136 PALSLPKLSSAESPKLPNPGVVVSKSRSHGVQI 38 P+ S+PK ++P L N SKS S G + Sbjct: 191 PSNSIPKTEPLDTPSLSNSQERFSKSPSDGQNV 223 >SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 23.8 bits (49), Expect = 10.0 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -1 Query: 205 SRPFSILSSSIPWSAKLEISFT 140 ++P SI +S PWS + ++SF+ Sbjct: 301 NQPISIYIASGPWSLRDDLSFS 322 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 23.8 bits (49), Expect = 10.0 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = -1 Query: 232 IPPNEVAMPSRPFSILSSSIPWSAK 158 +P + + + PFS+LSS P S++ Sbjct: 835 VPGSTSPLNASPFSVLSSDFPGSSR 859 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 23.8 bits (49), Expect = 10.0 Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 2/67 (2%) Frame = -1 Query: 274 FAIFEVRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPK-LSSAES 98 F F R P P N ++PS P SSS P + + + + + +P L S++ Sbjct: 98 FKNFPFRPPTRLTSTPSNSSSLPSIP---SSSSTPSISSIPHTTSSVSNDIPSVLGSSDH 154 Query: 97 P-KLPNP 80 P L NP Sbjct: 155 PIDLDNP 161 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,538,730 Number of Sequences: 5004 Number of extensions: 30794 Number of successful extensions: 97 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 97 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 134126124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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