BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31703
(400 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.35
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 27 1.4
SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 4.3
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 5.7
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 25 5.7
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb... 25 5.7
SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 24 10.0
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 24 10.0
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 24 10.0
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.7 bits (61), Expect = 0.35
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -1
Query: 250 PPMAVPIPPNEVAMPSRPFSILSSSIPWSA 161
PPM+VP PP+ MP+ P S +P S+
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 26.6 bits (56), Expect = 1.4
Identities = 21/74 (28%), Positives = 31/74 (41%)
Frame = -1
Query: 229 PPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPGVVVSKSRSH 50
P + V + S LSSS+ S + PA S K E+P P P V S SR
Sbjct: 227 PGSFVYEDAEKLSQLSSSLISSFSEQPKEHSPATS--KHEPEETPASPTPSVSASTSRER 284
Query: 49 GVQIPESMVSQXRS 8
+ S ++ ++
Sbjct: 285 STSVAPSFITSDQA 298
>SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 700
Score = 25.0 bits (52), Expect = 4.3
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 256 RAPPMAVPIPPNEVAMPSRPFS 191
R P M +P PP++ +PS P S
Sbjct: 24 RQPSMNLPTPPHDDGLPSIPRS 45
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 24.6 bits (51), Expect = 5.7
Identities = 14/52 (26%), Positives = 27/52 (51%)
Frame = -1
Query: 247 PMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPK 92
P + P PP + + S+P S SS++P K F+ + S ++++ P+
Sbjct: 193 PYSSPFPPPRIVLGSKPVS--SSTLPSKPKSFQVFSDASSSRDSQNASDLPQ 242
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 24.6 bits (51), Expect = 5.7
Identities = 25/76 (32%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = -1
Query: 253 APPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPGV 74
AP + + P + +P RP LS E+S PAL+ LSS SP P P
Sbjct: 347 APASEMSLIPEKPRLPPRPSHTLS---------ELS--SPALTSENLSSKPSPLFPPPPP 395
Query: 73 -VVSKSRSHGVQIPES 29
V S + + V +P S
Sbjct: 396 RVKSLATNKPVSMPVS 411
>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 386
Score = 24.6 bits (51), Expect = 5.7
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 136 PALSLPKLSSAESPKLPNPGVVVSKSRSHGVQI 38
P+ S+PK ++P L N SKS S G +
Sbjct: 191 PSNSIPKTEPLDTPSLSNSQERFSKSPSDGQNV 223
>SPCC553.09c |spb70|pol12|DNA polymerase alpha
B-subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 23.8 bits (49), Expect = 10.0
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -1
Query: 205 SRPFSILSSSIPWSAKLEISFT 140
++P SI +S PWS + ++SF+
Sbjct: 301 NQPISIYIASGPWSLRDDLSFS 322
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 23.8 bits (49), Expect = 10.0
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -1
Query: 232 IPPNEVAMPSRPFSILSSSIPWSAK 158
+P + + + PFS+LSS P S++
Sbjct: 835 VPGSTSPLNASPFSVLSSDFPGSSR 859
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 23.8 bits (49), Expect = 10.0
Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 2/67 (2%)
Frame = -1
Query: 274 FAIFEVRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPK-LSSAES 98
F F R P P N ++PS P SSS P + + + + + +P L S++
Sbjct: 98 FKNFPFRPPTRLTSTPSNSSSLPSIP---SSSSTPSISSIPHTTSSVSNDIPSVLGSSDH 154
Query: 97 P-KLPNP 80
P L NP
Sbjct: 155 PIDLDNP 161
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,538,730
Number of Sequences: 5004
Number of extensions: 30794
Number of successful extensions: 97
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 97
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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