BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31684 (500 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g57870.1 68416.m06451 ubiquitin-conjugating enzyme, putative ... 27 5.4 At3g13600.1 68416.m01712 calmodulin-binding family protein conta... 27 7.1 >At3g57870.1 68416.m06451 ubiquitin-conjugating enzyme, putative strong similarity to SP|P50550 Ubiquitin-like protein SUMO-1 conjugating enzyme (EC 6.3.2.19) (SUMO- 1-protein ligase) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme UbcE2A) {Xenopus laevis}; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 160 Score = 27.5 bits (58), Expect = 5.4 Identities = 13/52 (25%), Positives = 25/52 (48%) Frame = -1 Query: 200 TPCWRLRNLQFSFKQSFHIKQLTVGSLDIVQSVQMEQYFQITYYYLTIEHPV 45 T C + N + ++ + +KQ+ VG D++ + Q Y+L + PV Sbjct: 92 TVCLSILNEDYGWRPAITVKQILVGIQDLLDTPNPADPAQTDGYHLFCQDPV 143 >At3g13600.1 68416.m01712 calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 605 Score = 27.1 bits (57), Expect = 7.1 Identities = 17/37 (45%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -3 Query: 183 KKSSI*F*TIFPHQTTHSRFSRHRTECSN-GAVFSNN 76 K+SSI F I H+T SR+SR RT + G S N Sbjct: 146 KRSSISFFDIEKHETAISRWSRARTRAAKVGKGLSKN 182 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,081,529 Number of Sequences: 28952 Number of extensions: 161037 Number of successful extensions: 229 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 226 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 229 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 888318720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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