SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31679
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    71   8e-14
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    71   1e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    54   2e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    34   0.015
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    33   0.019
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    25   6.7  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 71.3 bits (167), Expect = 8e-14
 Identities = 43/110 (39%), Positives = 60/110 (54%), Gaps = 6/110 (5%)
 Frame = +1

Query: 205 KQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMVGE----ASGPINFT 372
           +Q AE +EAF L D D+DG I  N+L     SLG      EL +M+ E     +G I+FT
Sbjct: 9   EQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFT 68

Query: 373 QLLTXFANRMSGGSDEXDVVINAFKTFXEEGK--IDSERLTHALMTWGDK 516
           + LT  A +M   +D  + V  AFK F ++G   I  E LTH L + G++
Sbjct: 69  EFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117



 Score = 32.3 bits (70), Expect = 0.044
 Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
 Frame = +1

Query: 217 EXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMVGEA----SGPINFTQ 375
           E +EAF + D D +G I   +L     SLG     +E+ +M+ EA     G IN+ +
Sbjct: 86  EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEE 142


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 70.5 bits (165), Expect = 1e-13
 Identities = 41/123 (33%), Positives = 61/123 (49%), Gaps = 2/123 (1%)
 Frame = +1

Query: 154 SRKAKRTGSNVFSMFSXKQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELD 333
           ++ AKR  S  F+  +  Q  E KEAF L+D D DG IG+ D++    SL   A +  ++
Sbjct: 28  AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87

Query: 334 EMVGEASGPINFTQLLTXFANRMSGGSDEXDVVINAFKTF--XEEGKIDSERLTHALMTW 507
            M    + PIN    LT   + +   S   D ++ AF TF   + GKI    +  AL + 
Sbjct: 88  HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146

Query: 508 GDK 516
           GD+
Sbjct: 147 GDR 149


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 53.6 bits (123), Expect = 2e-08
 Identities = 33/108 (30%), Positives = 56/108 (51%), Gaps = 2/108 (1%)
 Frame = +1

Query: 199 SXKQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMVGEASGPINFTQL 378
           S +Q  E KEAF L D DKDG+I  + + +   SLG    D EL ++  E    I+  + 
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 379 LTXFANRMSGGSDEXDVVINAFKTFXEE--GKIDSERLTHALMTWGDK 516
           ++  +N++     E +  I AF+ F ++  G I++ +    + T G+K
Sbjct: 64  MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEK 110


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 33.9 bits (74), Expect = 0.015
 Identities = 17/42 (40%), Positives = 23/42 (54%)
 Frame = +1

Query: 205 KQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKEL 330
           +Q  +  EAF L D DKD  I  ++LRA   +LG  A   E+
Sbjct: 34  EQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEV 75


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 33.5 bits (73), Expect = 0.019
 Identities = 15/42 (35%), Positives = 24/42 (57%)
 Frame = +1

Query: 217 EXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMV 342
           E  + F + D D  G+IG  +LR    SLG    ++E+DE++
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 15/42 (35%), Positives = 20/42 (47%)
 Frame = +1

Query: 217 EXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMV 342
           E +EAF L D    G I   DLR +   LG     ++L  M+
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.315    0.132    0.370 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,381,548
Number of Sequences: 5004
Number of extensions: 17334
Number of successful extensions: 62
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 62
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)

- SilkBase 1999-2023 -