BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31679 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 71 8e-14 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 71 1e-13 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 54 2e-08 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 34 0.015 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 33 0.019 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 25 6.7 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 71.3 bits (167), Expect = 8e-14 Identities = 43/110 (39%), Positives = 60/110 (54%), Gaps = 6/110 (5%) Frame = +1 Query: 205 KQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMVGE----ASGPINFT 372 +Q AE +EAF L D D+DG I N+L SLG EL +M+ E +G I+FT Sbjct: 9 EQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFT 68 Query: 373 QLLTXFANRMSGGSDEXDVVINAFKTFXEEGK--IDSERLTHALMTWGDK 516 + LT A +M +D + V AFK F ++G I E LTH L + G++ Sbjct: 69 EFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117 Score = 32.3 bits (70), Expect = 0.044 Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 4/57 (7%) Frame = +1 Query: 217 EXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMVGEA----SGPINFTQ 375 E +EAF + D D +G I +L SLG +E+ +M+ EA G IN+ + Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEE 142 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 70.5 bits (165), Expect = 1e-13 Identities = 41/123 (33%), Positives = 61/123 (49%), Gaps = 2/123 (1%) Frame = +1 Query: 154 SRKAKRTGSNVFSMFSXKQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELD 333 ++ AKR S F+ + Q E KEAF L+D D DG IG+ D++ SL A + ++ Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87 Query: 334 EMVGEASGPINFTQLLTXFANRMSGGSDEXDVVINAFKTF--XEEGKIDSERLTHALMTW 507 M + PIN LT + + S D ++ AF TF + GKI + AL + Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146 Query: 508 GDK 516 GD+ Sbjct: 147 GDR 149 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 53.6 bits (123), Expect = 2e-08 Identities = 33/108 (30%), Positives = 56/108 (51%), Gaps = 2/108 (1%) Frame = +1 Query: 199 SXKQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMVGEASGPINFTQL 378 S +Q E KEAF L D DKDG+I + + + SLG D EL ++ E I+ + Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63 Query: 379 LTXFANRMSGGSDEXDVVINAFKTFXEE--GKIDSERLTHALMTWGDK 516 ++ +N++ E + I AF+ F ++ G I++ + + T G+K Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEK 110 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 33.9 bits (74), Expect = 0.015 Identities = 17/42 (40%), Positives = 23/42 (54%) Frame = +1 Query: 205 KQXAEXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKEL 330 +Q + EAF L D DKD I ++LRA +LG A E+ Sbjct: 34 EQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEV 75 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 33.5 bits (73), Expect = 0.019 Identities = 15/42 (35%), Positives = 24/42 (57%) Frame = +1 Query: 217 EXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMV 342 E + F + D D G+IG +LR SLG ++E+DE++ Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 25.0 bits (52), Expect = 6.7 Identities = 15/42 (35%), Positives = 20/42 (47%) Frame = +1 Query: 217 EXKEAFXLMDHDKDGIIGKNDLRATFDSLGXLAXDKELDEMV 342 E +EAF L D G I DLR + LG ++L M+ Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.315 0.132 0.370 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,381,548 Number of Sequences: 5004 Number of extensions: 17334 Number of successful extensions: 62 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 58 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 62 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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