Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31678
(357 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to calm... 52 9e-08
At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to calm... 52 9e-08
At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to calmod... 52 1e-07
At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to ca... 52 1e-07
At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to... 52 1e-07
At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost i... 52 1e-07
At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identica... 52 1e-07
At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identica... 52 1e-07
At4g14640.1 68417.m02252 calmodulin-8 (CAM8) identical to calmod... 46 6e-06
At3g22930.1 68416.m02889 calmodulin, putative strong similarity ... 44 4e-05
At1g12310.1 68414.m01423 calmodulin, putative similar to calmodu... 39 0.001
At1g62820.1 68414.m07092 calmodulin, putative similar to calmodu... 38 0.002
At1g24620.1 68414.m03097 polcalcin, putative / calcium-binding p... 37 0.005
At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identica... 33 0.042
At5g37770.1 68418.m04547 touch-responsive protein / calmodulin-r... 31 0.17
At5g17470.1 68418.m02050 calmodulin-related protein, putative si... 31 0.30
At3g50360.1 68416.m05507 caltractin / centrin identical to caltr... 31 0.30
At3g63150.1 68416.m07092 GTP-binding protein-related low similar... 29 0.69
At2g36180.1 68415.m04440 calmodulin-related protein, putative si... 29 1.2
At3g55400.1 68416.m06153 methionyl-tRNA synthetase / methionine-... 28 1.6
At3g03430.1 68416.m00341 polcalcin, putative / calcium-binding p... 28 2.1
At4g37010.1 68417.m05243 caltractin, putative / centrin, putativ... 26 6.4
At4g18490.1 68417.m02742 hypothetical protein 26 6.4
At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative / phos... 26 8.5
>At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to
calmodulin 4 [Arabidopsis thaliana] GI:16223, SP|P25854
Calmodulin-1/4 {Arabidopsis thaliana}
Length = 149
Score = 52.4 bits (120), Expect = 9e-08
Identities = 33/109 (30%), Positives = 51/109 (46%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ AKK KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to
calmodulin [Arabidopsis thaliana] GI:16223; nearly
identical to SP|P25854 Calmodulin-1/4 {Arabidopsis
thaliana}
Length = 149
Score = 52.4 bits (120), Expect = 9e-08
Identities = 33/109 (30%), Positives = 51/109 (46%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ AKK KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to
calmodulin-6 SP:Q03509 from [Arabidopsis thaliana];
contains Pfam profile: PF00036 EF hand
Length = 149
Score = 51.6 bits (118), Expect = 1e-07
Identities = 32/109 (29%), Positives = 52/109 (47%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to
calmodulin GI:474183 from [Arabidopsis thaliana]; almost
identical to calmodulin-2/3/5 SP:P25069 [Arabidopsis
thaliana]
Length = 149
Score = 51.6 bits (118), Expect = 1e-07
Identities = 32/109 (29%), Positives = 52/109 (47%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to
calmodulin GI:16227 from [Arabidopsis thaliana],
SP|P59220 Calmodulin-7 {Arabidopsis thaliana}
Length = 149
Score = 51.6 bits (118), Expect = 1e-07
Identities = 32/109 (29%), Positives = 52/109 (47%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost
identical to Calmodulin-2/3/5 SP:P25069 from
[Arabidopsis thaliana]
Length = 149
Score = 51.6 bits (118), Expect = 1e-07
Identities = 32/109 (29%), Positives = 52/109 (47%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 181
Score = 51.6 bits (118), Expect = 1e-07
Identities = 32/109 (29%), Positives = 52/109 (47%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 149
Score = 51.6 bits (118), Expect = 1e-07
Identities = 32/109 (29%), Positives = 52/109 (47%), Gaps = 1/109 (0%)
Frame = +2
Query: 32 MSD-LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXX 208
M+D L+ + + AFS++D +G G I LG ++R+L NPT A +
Sbjct: 1 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60
Query: 209 XXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 61 NGTIDFPEF-LNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 108
>At4g14640.1 68417.m02252 calmodulin-8 (CAM8) identical to
calmodulin 8 GI:5825600 from [Arabidopsis thaliana]
Length = 151
Score = 46.4 bits (105), Expect = 6e-06
Identities = 28/105 (26%), Positives = 49/105 (46%)
Frame = +2
Query: 41 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXX 220
L+K+ + AF ++D +G G I L ++R+L+ NPT +
Sbjct: 6 LTKDQITEFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTI 65
Query: 221 XXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ AKK ++ A E+ E K+ DK++NG + +EL+H
Sbjct: 66 EFAEF-LNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELSH 109
>At3g22930.1 68416.m02889 calmodulin, putative strong similarity to
calmodulin 8 GI:5825600 from [Arabidopsis thaliana];
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 173
Score = 43.6 bits (98), Expect = 4e-05
Identities = 27/114 (23%), Positives = 51/114 (44%)
Frame = +2
Query: 14 RQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXX 193
+Q + +L++ + AF ++D +G G I A L ++R+L+ NPT +
Sbjct: 19 QQQQQQQQELTQEQIMEFKEAFCLFDKDGDGCITADELATVIRSLDQNPTEQELQDMITE 78
Query: 194 XXXXXXXXXXXXXXPIYSQAKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
+ A + ++ A E+ E K+ DK++NG + +EL H
Sbjct: 79 IDSDGNGTIEFSEF-LNLMANQLQETDADEELKEAFKVFDKDQNGYISASELRH 131
>At1g12310.1 68414.m01423 calmodulin, putative similar to calmodulin
SP:P04465 from [Trypanosoma brucei gambiense]
Length = 148
Score = 38.7 bits (86), Expect = 0.001
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +2
Query: 41 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 175
LS + V AF ++D +G GKI LG L+R+L NPT A +
Sbjct: 6 LSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQL 50
>At1g62820.1 68414.m07092 calmodulin, putative similar to calmodulin
SP:P04465 from [Trypanosoma brucei gambiense]; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 148
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/41 (43%), Positives = 24/41 (58%)
Frame = +2
Query: 41 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPT 163
LS + V AF ++D +G GKI LG L+R+L NPT
Sbjct: 6 LSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPT 46
>At1g24620.1 68414.m03097 polcalcin, putative / calcium-binding
pollen allergen, putative similar to polcalcin Jun o 2
(calcium-binding pollen allergen Jun o 2) SP:O64943 from
[Juniperus oxycedrus]
Length = 186
Score = 36.7 bits (81), Expect = 0.005
Identities = 18/44 (40%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Frame = +2
Query: 41 LSKNDV-ERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLA 169
+ +NDV E AFS+YD +G G I A L ++LR+L ++A
Sbjct: 102 MDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIA 145
>At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 113
Score = 33.5 bits (73), Expect = 0.042
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 251 AKKDKDXGAYEDFLECLKLXDKNENGLMLGAELTH 355
A+K KD + E+ E ++ DK++NG + AEL H
Sbjct: 38 ARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRH 72
>At5g37770.1 68418.m04547 touch-responsive protein /
calmodulin-related protein 2, touch-induced (TCH2)
identical to calmodulin-related protein 2,touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 161
Score = 31.5 bits (68), Expect = 0.17
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +2
Query: 44 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRAL 148
++NDV AF +YD +G G+I A L +++ L
Sbjct: 88 NRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNL 122
>At5g17470.1 68418.m02050 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 146
Score = 30.7 bits (66), Expect = 0.30
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +2
Query: 74 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLA 169
AF +YD +G GKI A + +L+ L T+A
Sbjct: 81 AFDLYDIDGDGKISASEIHVVLKRLGEKQTIA 112
>At3g50360.1 68416.m05507 caltractin / centrin identical to
caltractin; centrin GI:3688162 from [Arabidopsis
thaliana]
Length = 169
Score = 30.7 bits (66), Expect = 0.30
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +2
Query: 14 RQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPT 163
++ P + L+ + AF ++D +G G IDA L +RAL T
Sbjct: 11 KEKPRRHHGLTTQKKQEIKEAFELFDTDGSGTIDAKELNVAMRALGFEMT 60
>At3g63150.1 68416.m07092 GTP-binding protein-related low similarity
to SP|Q38912 RAC-like GTP binding protein ARAC3
(GTP-binding protein ROP6) {Arabidopsis thaliana};
contains Pfam profile PF00036: EF hand (domain)
Length = 643
Score = 29.5 bits (63), Expect = 0.69
Identities = 13/51 (25%), Positives = 25/51 (49%)
Frame = +2
Query: 14 RQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTL 166
+Q+P + +L+ ++ S F +YD + G + L DL + +P L
Sbjct: 303 KQSPDQSIELTNEAMDFLSGIFQLYDLDNDGALQPAELDDLFQTAPDSPWL 353
>At2g36180.1 68415.m04440 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 144
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 74 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTL 166
AF +YD +G GKI A + +L+ L T+
Sbjct: 80 AFDLYDMDGDGKISASEIHVVLKRLGEKHTM 110
>At3g55400.1 68416.m06153 methionyl-tRNA synthetase /
methionine--tRNA ligase / MetRS (cpMetRS) identical to
methionyl-tRNA synthetase MEtRS [Arabidopsis thaliana]
GI:2266985
Length = 616
Score = 28.3 bits (60), Expect = 1.6
Identities = 20/67 (29%), Positives = 31/67 (46%)
Frame = +3
Query: 9 GTDKHHTK*ATSARTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSV 188
GTD+H K ATSA GR P + R++ L I+ ++ +T P ++
Sbjct: 114 GTDEHGEKIATSA--AANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTT--DPKHEAI 169
Query: 189 VQRXXAR 209
V+ AR
Sbjct: 170 VKEFYAR 176
>At3g03430.1 68416.m00341 polcalcin, putative / calcium-binding
pollen allergen, putative almost identical to polcalcin
Bra r 2/Bra n 2 (calcium-binding pollen allergen Bra r
2/Bra n 2) SP:Q39406 from [Brassica napus]
Length = 83
Score = 27.9 bits (59), Expect = 2.1
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +2
Query: 77 FSIYDFEGKGKIDAFNLGDLLRALNS 154
F +D G GKI A LGD L+ L S
Sbjct: 14 FKKFDANGDGKISAAELGDALKNLGS 39
>At4g37010.1 68417.m05243 caltractin, putative / centrin, putative
similar to Caltractin (Centrin) SP:P41210 from [Atriplex
nummularia]
Length = 167
Score = 26.2 bits (55), Expect = 6.4
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +2
Query: 77 FSIYDFEGKGKIDAFNLGDLLRAL 148
F ++D +G G IDA L +R+L
Sbjct: 32 FDLFDIDGSGSIDASELNVAMRSL 55
>At4g18490.1 68417.m02742 hypothetical protein
Length = 594
Score = 26.2 bits (55), Expect = 6.4
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 51 TTLKGRLSPSQSTTLKAKVRSMPSTLAI 134
TTL G++SP T+ AKV+ + L +
Sbjct: 262 TTLSGKISPGTRTSQTAKVQDLSEKLPL 289
>At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to phosphatidylinositol
transfer-like protein IV (GI:14486707) [Lotus japonicus]
Length = 631
Score = 25.8 bits (54), Expect = 8.5
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = +1
Query: 124 PWRSPESAQLKPHTGNHRETRWYKEXXREAAHTRRVXSHLQPS 252
PW++PE ++ H G HR + K E +V ++ +PS
Sbjct: 341 PWKNPEIVKMVLHGGAHRARQVVKVLNSEG----KVIAYAKPS 379
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,441,950
Number of Sequences: 28952
Number of extensions: 95390
Number of successful extensions: 290
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 278
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 282
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 459356736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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