BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31677 (400 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 56 2e-09 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 29 0.27 SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein L15b|Schizos... 28 0.46 SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces... 28 0.61 SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 27 1.4 SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 27 1.4 SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr... 24 7.6 >SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 56.0 bits (129), Expect = 2e-09 Identities = 27/74 (36%), Positives = 44/74 (59%), Gaps = 2/74 (2%) Frame = +2 Query: 179 WEKXHKKTFTAWCNSHLRKAGT-GIENIEDDFRNGLKLMLLLEVIXGETLPKPDRG-KMR 352 W+ +TFT W N+ L + ++ D +G+ L+ LLE+I E L + +R +MR Sbjct: 6 WQSVQNRTFTKWFNTKLSSRDLPSVFDLRKDLSDGILLIQLLEIIGDENLGRYNRNPRMR 65 Query: 353 FHKIANVNKALDFI 394 H++ NVNKAL++I Sbjct: 66 VHRLENVNKALEYI 79 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 29.1 bits (62), Expect = 0.27 Identities = 17/46 (36%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = +2 Query: 260 EDDFRNGLKLMLLLEVIXGETLPKPD-RGKMRFHKIANVNKALDFI 394 E RNG+ L LL++ + L K +++F N+NK LDFI Sbjct: 66 EQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFI 111 >SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein L15b|Schizosaccharomyces pombe|chr 1|||Manual Length = 201 Score = 28.3 bits (60), Expect = 0.46 Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 5/51 (9%) Frame = +1 Query: 205 HSXVQQSPQKSW-----HRHREHRGRLPQWXEAHVAAGGHLRRDSAQARPW 342 H +++ P+ +W H+HRE RG ++ GH +S Q W Sbjct: 139 HKAIRRDPRINWIVNPVHKHRESRGLTSIGKKSRGIGKGHRYNNSPQHATW 189 >SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces pombe|chr 3|||Manual Length = 201 Score = 27.9 bits (59), Expect = 0.61 Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 5/51 (9%) Frame = +1 Query: 205 HSXVQQSPQKSW-----HRHREHRGRLPQWXEAHVAAGGHLRRDSAQARPW 342 H +++ P+ +W H+HRE RG ++ GH +S Q W Sbjct: 139 HKAIRRDPRINWIVNPVHKHRESRGLTSIGKKSRGIGKGHRFNNSPQHATW 189 >SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 26.6 bits (56), Expect = 1.4 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +1 Query: 283 EAHVAAGGHLRRDSAQARPW 342 EAHVAA +LR+ RPW Sbjct: 250 EAHVAALDYLRQHFVSCRPW 269 >SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 218 Score = 26.6 bits (56), Expect = 1.4 Identities = 11/35 (31%), Positives = 17/35 (48%) Frame = +2 Query: 155 REGLLHPAWEKXHKKTFTAWCNSHLRKAGTGIENI 259 REG+ P WE H KT W + + G ++ + Sbjct: 97 REGV-RPEWEDVHNKTGGKWAFQNKGRGGNALDEM 130 >SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 473 Score = 24.2 bits (50), Expect = 7.6 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = -1 Query: 79 SYMVTALTVDSMFVRAYDR 23 S VTAL DS F +AY+R Sbjct: 178 SLAVTALEHDSAFAKAYER 196 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,423,746 Number of Sequences: 5004 Number of extensions: 25225 Number of successful extensions: 78 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 77 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 134126124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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