Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31663
(490 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 72 4e-14
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 58 9e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 47 1e-06
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 38 8e-04
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 38 0.001
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 30 0.22
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 0.66
SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 2.0
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 27 2.0
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 26 2.7
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 2.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 2.7
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 2.7
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 26 3.5
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 3.5
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 26 3.5
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 25 4.6
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 25 8.1
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 25 8.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 72.1 bits (169), Expect = 4e-14
Identities = 45/133 (33%), Positives = 71/133 (53%), Gaps = 6/133 (4%)
Frame = +2
Query: 26 KQVAEFKGGIPANGITTRDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINF 193
+Q+AEF+ +DG I N+L SLG+ + EL +M+ E +G I+F
Sbjct: 9 EQIAEFREAFSLFD-RDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67
Query: 194 TQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDEA 367
T+ LT+ A +M +D ++ V AFK FD++G I E L H L + G++ S +EV +
Sbjct: 68 TEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADM 126
Query: 368 YDQMDIDDKGYID 406
+ D D G I+
Sbjct: 127 IREADTDGDGVIN 139
Score = 28.7 bits (61), Expect = 0.50
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +2
Query: 278 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 436
D G ID +E L D + +EV EA+ D D GYI +L +LT+
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 57.6 bits (133), Expect = 9e-10
Identities = 33/96 (34%), Positives = 50/96 (52%), Gaps = 2/96 (2%)
Frame = +2
Query: 80 DGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVI 259
DG IG+ D++ SL + ASE ++ M + PIN LT + + S +D ++
Sbjct: 62 DGNIGREDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRND-LL 120
Query: 260 NAFKTFD--EEGKIDSERLRHALMTWGDKFSADEVD 361
AF TFD + GKI +R AL + GD+ EV+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 47.2 bits (107), Expect = 1e-06
Identities = 29/122 (23%), Positives = 60/122 (49%), Gaps = 2/122 (1%)
Frame = +2
Query: 77 RDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVV 256
+DG+I + + + SLG ++ EL ++ E I+ + ++ +N++ E++
Sbjct: 22 KDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEEE-Y 80
Query: 257 INAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 430
I AF+ FD++ G I++ + + T G+K S +EV + D + G D + +
Sbjct: 81 IKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQRI 140
Query: 431 TA 436
A
Sbjct: 141 MA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 37.9 bits (84), Expect = 8e-04
Identities = 27/124 (21%), Positives = 57/124 (45%), Gaps = 6/124 (4%)
Frame = +2
Query: 77 RDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDE 244
+D I ++LRA +LG A + E+ +++ + G + + + ++ D
Sbjct: 50 KDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVE-RDP 108
Query: 245 DDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKL 418
+ + AF+ FD++ GKI LR + E++ ++ D+D G I+ +
Sbjct: 109 LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEF 168
Query: 419 IAML 430
IA++
Sbjct: 169 IAIM 172
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 37.5 bits (83), Expect = 0.001
Identities = 29/123 (23%), Positives = 57/123 (46%), Gaps = 4/123 (3%)
Frame = +2
Query: 83 GIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGS--DEDDVV 256
G I K + + G+ + E+ E+ ++ Q L + NR +G + +
Sbjct: 21 GRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NRPNGFDMPGDPEEF 79
Query: 257 INAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 430
+ F+ FD++ G I LR+ L + G+K S +E+DE + + D G ++ + M+
Sbjct: 80 VKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD-GMVNYHDFVQMI 138
Query: 431 TAS 439
A+
Sbjct: 139 LAN 141
>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1364
Score = 29.9 bits (64), Expect = 0.22
Identities = 16/40 (40%), Positives = 20/40 (50%)
Frame = +2
Query: 62 NGITTRDGIIGKNDLRATFDSLGRLASEKELDEMVGEASG 181
N I T D +IG+N R SLG + +EL E A G
Sbjct: 8 NSIATTDNVIGRNKSRKRPHSLGGPGALQELKEHTNPAKG 47
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 28.3 bits (60), Expect = 0.66
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +2
Query: 350 DEVDEAYDQMDIDDKGYID 406
+E +EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
>SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 231
Score = 26.6 bits (56), Expect = 2.0
Identities = 18/41 (43%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -2
Query: 159 SSSSFSDASLPRESKVARKSFLPMMPSLVVIPLA--GMPPL 43
SSSS S +S P + +RK P MP L LA PPL
Sbjct: 3 SSSSSSGSSSPASKRSSRKRQTPPMPELPSFLLAFPHAPPL 43
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 26.6 bits (56), Expect = 2.0
Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
Frame = -2
Query: 240 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 79
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S P PS
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595
>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
Msa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 26.2 bits (55), Expect = 2.7
Identities = 23/63 (36%), Positives = 30/63 (47%)
Frame = -2
Query: 264 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 85
A I S SS+PP R S V+ A ++S+ FS P ES + S LP +
Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326
Query: 84 PSL 76
PSL
Sbjct: 327 PSL 329
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 26.2 bits (55), Expect = 2.7
Identities = 20/75 (26%), Positives = 33/75 (44%)
Frame = -2
Query: 255 TTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSL 76
++SSSS ++ S V + +S SSSS S +S + SF+ M S
Sbjct: 188 SSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSFITTMSSS 247
Query: 75 VVIPLAGMPPLNSAT 31
I + P +S++
Sbjct: 248 TFISTVTVTPSSSSS 262
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 26.2 bits (55), Expect = 2.7
Identities = 15/57 (26%), Positives = 24/57 (42%)
Frame = +2
Query: 26 KQVAEFKGGIPANGITTRDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFT 196
K + G +P +TT+ K +L FD+ +E ++ V E S FT
Sbjct: 766 KFLGSLAGNLPQESLTTKSKRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLYPFT 822
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 26.2 bits (55), Expect = 2.7
Identities = 21/65 (32%), Positives = 27/65 (41%), Gaps = 1/65 (1%)
Frame = -1
Query: 196 SEVDGARSLTDHLIELLLGR-EPAQGVEGGAQVVLADDAIPCRDPISWNASLELGDLLXV 20
+ V ++ H IEL R + AQ V DD P S SL GDL
Sbjct: 82 TSVTAIENVVKHFIELANKRVQEAQEKADKISVEYVDDLEATETPESIMMSLVSGDLSKS 141
Query: 19 RTERS 5
RT+R+
Sbjct: 142 RTDRA 146
>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1014
Score = 25.8 bits (54), Expect = 3.5
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 65 GITTRDGIIGKNDLRATFDSLGRL 136
G+T DG + + LR+ F+S G+L
Sbjct: 877 GVTNVDGTVNEARLRSLFESYGKL 900
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 3.5
Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = -2
Query: 174 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 79
+SP++ +++ + P++S V+RKSF P P+
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 25.8 bits (54), Expect = 3.5
Identities = 11/48 (22%), Positives = 21/48 (43%)
Frame = -2
Query: 186 MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSLVVIPLAGMPPL 43
M P+ P + F + R + F+P M ++ + G+PP+
Sbjct: 644 MAPQFIPPYAMPQFPPSGNGRGASAPATYFVPQMGGMMAYTMNGVPPM 691
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 25.4 bits (53), Expect = 4.6
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = -2
Query: 147 FSDASLPRESKVARKSFLPMMPSLVVIPLAGMPPLNS 37
F E KV +FLP++ L ++ A PPL S
Sbjct: 11 FDSYDFKHEGKVHLSNFLPIINDLQLLHPASAPPLLS 47
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 24.6 bits (51), Expect = 8.1
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 278 DEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 391
D++ K+ + LR T D DEVD Y+++ +DD
Sbjct: 103 DQKSKV-IQCLRTERKTIPDDLVEDEVDRFYNKLGLDD 139
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 24.6 bits (51), Expect = 8.1
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 278 DEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDK 394
DEE +I S R+ T+ S E+DE + +DI +
Sbjct: 524 DEESRIGSSSTRNDNSTFQTSDSFQELDERTNLLDISKR 562
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,510,779
Number of Sequences: 5004
Number of extensions: 25104
Number of successful extensions: 137
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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