BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31661 (403 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 31 0.067 SPBC119.16c |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.4 SPBC651.10 |nse5||Smc5-6 complex non-SMC subunit Nse5|Schizosacc... 26 1.9 SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 25 4.4 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 31.1 bits (67), Expect = 0.067 Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 2/58 (3%) Frame = -1 Query: 259 PLFAPFVXVFLQLFIQVRPLLVLTLDEFWISLTLLFRSG*HRDFFF--YFLLLRARIF 92 P+FA + L LF+Q+ P + + FW S+ L+ +G +FF Y + A+I+ Sbjct: 796 PIFAYVISKCLNLFMQIDPSIGVA---FWSSMVLVVAAGSGASYFFSHYIFSISAKIW 850 >SPBC119.16c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 26.6 bits (56), Expect = 1.4 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = -1 Query: 121 YFLLLRARIFLLVSTCWLFI 62 YF L R + L STC+LFI Sbjct: 424 YFWLRRVSLIFLASTCFLFI 443 >SPBC651.10 |nse5||Smc5-6 complex non-SMC subunit Nse5|Schizosaccharomyces pombe|chr 2|||Manual Length = 388 Score = 26.2 bits (55), Expect = 1.9 Identities = 14/26 (53%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Frame = -3 Query: 266 PWPAVCAIRXCIPSTVHPS-PTASGL 192 P PA C IR CI VHP PT+S + Sbjct: 35 PIPA-CYIRMCITLLVHPEYPTSSNI 59 >SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 25.0 bits (52), Expect = 4.4 Identities = 11/20 (55%), Positives = 16/20 (80%) Frame = +2 Query: 176 EFIKRQDQKRSDLDEQLKEY 235 E +KR+DQK+ D+ E L+EY Sbjct: 272 ELLKRRDQKQQDV-EALQEY 290 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,073,463 Number of Sequences: 5004 Number of extensions: 16580 Number of successful extensions: 49 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 47 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 49 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 136158338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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