Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31654
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein...    78   7e-16
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      65   5e-12
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    38   7e-04
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos...    27   2.2  
SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   2.9  
SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po...    26   2.9  
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi...    26   3.8  
SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|...    25   5.1  
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   6.7  
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom...    25   6.7  
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||...    25   6.7  
SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch...    25   6.7  
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M...    25   6.7  
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo...    25   8.9  
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy...    25   8.9  
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|...    25   8.9  

>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
           Stg1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 174

 Score = 78.2 bits (184), Expect = 7e-16
 Identities = 55/146 (37%), Positives = 77/146 (52%), Gaps = 4/146 (2%)
 Frame = +3

Query: 66  EQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 239
           E+E   WI + L   L N   +  D L+ GVILC++  K A G+  + +E    F  MEN
Sbjct: 6   EKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMEN 63

Query: 240 IQRF-QAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKM 416
           I  F   A +   VP +++FQT+DLFERRN  QV   +++  R   K        LGPK+
Sbjct: 64  ISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKL 123

Query: 417 ADKNERTFTEEQLRAHNAELN-LQMG 491
           A+K  R F+ +Q R     +N LQ G
Sbjct: 124 AEKKPRVFSAQQQREFREGVNSLQYG 149


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 65.3 bits (152), Expect = 5e-12
 Identities = 35/98 (35%), Positives = 52/98 (53%), Gaps = 2/98 (2%)
 Frame = +3

Query: 72  EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 245
           E   WI + LG  L P   +E  LR+GV+L  L  K  P  + KI       F+  +NI 
Sbjct: 46  EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105

Query: 246 RFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 359
           +F   I   G+PE   F+  D++E +N+P+V  C++AL
Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 38.3 bits (85), Expect = 7e-04
 Identities = 30/101 (29%), Positives = 45/101 (44%), Gaps = 5/101 (4%)
 Frame = +3

Query: 72  EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 239
           E   W+ +       N   + D L +G +LC+LA K  P      + R   +Q+ E    
Sbjct: 69  EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125

Query: 240 -IQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 359
            +  F   +   G+     F+T DL  R NIP+V  CL+AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166


>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
           Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -3

Query: 235 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 113
           S+++KL  +      DP    LAN +++  SLS  +Y P G
Sbjct: 33  SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73


>SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 163

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +2

Query: 152 DPLQIGQQISSWISEKNPRERNQLPAHG 235
           D LQ+ ++  SWI+EK   E  +L + G
Sbjct: 11  DSLQVDEKAKSWIAEKVEEELRRLESVG 38


>SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 452

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 17/64 (26%), Positives = 27/64 (42%)
 Frame = -3

Query: 220 LVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLFLAGI 41
           L+ ++W    +P   +   +Q++ PS+ T   A      P   E         + FL GI
Sbjct: 257 LMHMAWALLDNPNLFVEPYVQQLMPSILTCLVAKRLGSDPNNHEHYALRDL--AAFLLGI 314

Query: 40  VCRR 29
           VC R
Sbjct: 315 VCDR 318


>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 749

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = -3

Query: 172 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 83
           L+N   +TPS S S Y P      +T  +Q
Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718


>SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 623

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 8/18 (44%), Positives = 11/18 (61%)
 Frame = -3

Query: 346 HNVTWGMLRLSKRSAVWN 293
           HN  WG  R  + +A+WN
Sbjct: 491 HNFVWGRFRELEHAAMWN 508


>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 780

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/39 (28%), Positives = 23/39 (58%)
 Frame = -3

Query: 172 LANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSL 56
           L++   I+  L  ++ +PF S S   +E++ RTS ++ +
Sbjct: 26  LSSYTSISRELDYANQSPFSSNSFSPTELKARTSATFDV 64


>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = +1

Query: 364 ELLKNIPSLQDLS*DLKWLIRTNVPSRKNNFGPTTLNLTSK 486
           EL+ N PS  D +  L + +   VP+ +  FG  +  + +K
Sbjct: 564 ELIPNDPSDDDYAAALNYAVNEYVPTYRKTFGNDSKKIKNK 604


>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1101

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = +1

Query: 211 EEPTSSSWRTFKGSKRQSRNTEYQKKKYSKPPIF 312
           E+P++S W+T  GS+    ++  +KK  S  P F
Sbjct: 185 EDPSNSYWKTVIGSREMFEDS--RKKTSSPSPSF 216


>SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 701

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 19/68 (27%), Positives = 34/68 (50%)
 Frame = -2

Query: 443 REGTFVLISHFRS*LRSCKLGMFLSNSSQSVQT*CYLGNVTPLKKIGGLEYFFFWYSVFL 264
           +EGT  L +     ++S     +++ +S  V     + N T +K +GGLE F    +  L
Sbjct: 376 KEGTEKLFALMEE-IKSVNDNRYITETSNKV-----MENFTEIKNLGGLE-FLRSEASSL 428

Query: 263 DCRLEPLN 240
           DC ++ +N
Sbjct: 429 DCLIDKVN 436


>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 535

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 13/25 (52%), Positives = 15/25 (60%)
 Frame = -2

Query: 266 LDCRLEPLNVLHELEVGSSLLDFFH 192
           LD  LEPL  LHE    S+ L FF+
Sbjct: 238 LDAYLEPLVHLHEYSRRSNTLFFFY 262


>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
           Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 778

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 13/43 (30%), Positives = 22/43 (51%)
 Frame = -3

Query: 295 NISSSGTPYFLIAAWNL*MFSMSWKLVPLSWIFFTDPGANLLA 167
           N   +G PY  I AW   + S+   + P+ ++   + G +LLA
Sbjct: 136 NYKDNGVPYVTIRAWPALLSSL---VPPVVFLIMKESGYDLLA 175


>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2052

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 11/27 (40%), Positives = 13/27 (48%)
 Frame = +2

Query: 146  RCDPLQIGQQISSWISEKNPRERNQLP 226
            RC P Q G   S W +E  P   + LP
Sbjct: 1617 RCSPKQSGATRSVWFNELGPLTLSFLP 1643


>SPBC1826.01c |mot1||TATA-binding protein associated factor
           Mot1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1953

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -3

Query: 97  TSEIQVRTSCSWSLFLAGIVCRRNLV 20
           T  I V+TSCSW   L   +C +N++
Sbjct: 638 TKFISVQTSCSWITGLTLRLCFQNVL 663


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,408,707
Number of Sequences: 5004
Number of extensions: 52776
Number of successful extensions: 181
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)