BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31653 (516 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_57888| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.32 SB_9222| Best HMM Match : 7tm_1 (HMM E-Value=4.1e-06) 31 0.74 SB_46293| Best HMM Match : Keratin_B2 (HMM E-Value=0.00077) 30 0.98 SB_8282| Best HMM Match : NHL (HMM E-Value=9.2e-23) 28 4.0 SB_3680| Best HMM Match : Keratin_B2 (HMM E-Value=0.0024) 28 5.3 >SB_57888| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 565 Score = 31.9 bits (69), Expect = 0.32 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP+ A W HP+ A W+HP Sbjct: 218 HPNKTQANWYHPNKTQANWNHP 239 Score = 31.1 bits (67), Expect = 0.56 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W+HP A W+HP Sbjct: 178 HPTKTQANWNHPSKTRANWNHP 199 Score = 30.7 bits (66), Expect = 0.74 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W+HP A W+HP Sbjct: 78 HPTKTHANWNHPSKTRANWNHP 99 Score = 30.7 bits (66), Expect = 0.74 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP+ A W+HP A W HP Sbjct: 228 HPNKTQANWNHPTKTQANWYHP 249 Score = 29.9 bits (64), Expect = 1.3 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W+HP Sbjct: 528 HPTKTQANWCHPTKTQANWNHP 549 Score = 29.1 bits (62), Expect = 2.3 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP + W+HP Sbjct: 268 HPSKTQANWYHPSKTQSNWNHP 289 Score = 29.1 bits (62), Expect = 2.3 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 408 HPSKTHANWCHPTKTQANWCHP 429 Score = 29.1 bits (62), Expect = 2.3 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 418 HPTKTQANWCHPTKTQANWCHP 439 Score = 29.1 bits (62), Expect = 2.3 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 428 HPTKTQANWCHPTKTQANWCHP 449 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/23 (43%), Positives = 11/23 (47%) Frame = +1 Query: 403 SHPHTLPARWSHPHTLTAXWSHP 471 +HP A W HP A W HP Sbjct: 237 NHPTKTQANWYHPTKTQANWYHP 259 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 258 HPTKTQANWYHPSKTQANWYHP 279 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP T A W +P A W HP Sbjct: 308 HPTTTHANWCYPTKTQANWCHP 329 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 458 HPTKTQANWYHPSKTQANWYHP 479 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 468 HPSKTQANWYHPTKTQANWYHP 489 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 478 HPTKTQANWYHPSKTKANWYHP 499 Score = 28.7 bits (61), Expect = 3.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 518 HPTKTQANWYHPTKTQANWCHP 539 Score = 28.3 bits (60), Expect = 4.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 248 HPTKTQANWYHPTKTQANWYHP 269 Score = 28.3 bits (60), Expect = 4.0 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP T A W +P Sbjct: 298 HPTKTHANWCHPTTTHANWCYP 319 Score = 28.3 bits (60), Expect = 4.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 508 HPTKTQANWYHPTKTQANWYHP 529 Score = 27.9 bits (59), Expect = 5.3 Identities = 8/23 (34%), Positives = 13/23 (56%) Frame = +1 Query: 403 SHPHTLPARWSHPHTLTAXWSHP 471 +HP + + W HP+ A W +P Sbjct: 47 NHPIKIQSNWCHPNKTQANWCYP 69 Score = 27.1 bits (57), Expect = 9.2 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP PA HP+ A W HP Sbjct: 208 HPTKTPAYLCHPNKTQANWYHP 229 Score = 27.1 bits (57), Expect = 9.2 Identities = 9/22 (40%), Positives = 9/22 (40%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP W HP Sbjct: 338 HPTKTQANWYHPSKTQVNWYHP 359 Score = 27.1 bits (57), Expect = 9.2 Identities = 9/22 (40%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP + W HP Sbjct: 488 HPSKTKANWYHPIKTQSNWYHP 509 >SB_9222| Best HMM Match : 7tm_1 (HMM E-Value=4.1e-06) Length = 425 Score = 30.7 bits (66), Expect = 0.74 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = -2 Query: 452 VNVCGCDHLAGNVCGCDWHGNGRL 381 VN GC+ GN GC++ G+ R+ Sbjct: 381 VNTMGCEFQGGNTMGCEFQGDSRI 404 >SB_46293| Best HMM Match : Keratin_B2 (HMM E-Value=0.00077) Length = 677 Score = 30.3 bits (65), Expect = 0.98 Identities = 10/23 (43%), Positives = 12/23 (52%) Frame = +1 Query: 403 SHPHTLPARWSHPHTLTAXWSHP 471 +HP A W HP+ A W HP Sbjct: 282 NHPIKTQANWCHPNKTQANWCHP 304 Score = 30.3 bits (65), Expect = 0.98 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W+HP A W+HP Sbjct: 423 HPTKTHANWNHPSKTWANWNHP 444 Score = 29.9 bits (64), Expect = 1.3 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W+HP A W HP Sbjct: 383 HPTKTQANWNHPSKTQANWYHP 404 Score = 28.3 bits (60), Expect = 4.0 Identities = 9/21 (42%), Positives = 11/21 (52%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSH 468 HP A W+HP A W+H Sbjct: 313 HPTKTQANWNHPSKTQANWNH 333 Score = 28.3 bits (60), Expect = 4.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 473 HPAKTQANWYHPTKTQANWYHP 494 Score = 28.3 bits (60), Expect = 4.0 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP A W HP Sbjct: 649 HPTKTQANWYHPTKTQANWYHP 670 Score = 27.9 bits (59), Expect = 5.3 Identities = 9/23 (39%), Positives = 11/23 (47%) Frame = +1 Query: 403 SHPHTLPARWSHPHTLTAXWSHP 471 +HP A W HP + W HP Sbjct: 392 NHPSKTQANWYHPPKTQSNWYHP 414 Score = 27.5 bits (58), Expect = 6.9 Identities = 8/22 (36%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP+ A W H + + W HP Sbjct: 193 HPYKTQANWCHTSKIQSNWYHP 214 Score = 27.5 bits (58), Expect = 6.9 Identities = 9/22 (40%), Positives = 10/22 (45%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP A W HP + W HP Sbjct: 503 HPTKTQANWYHPSKTQSNWYHP 524 >SB_8282| Best HMM Match : NHL (HMM E-Value=9.2e-23) Length = 877 Score = 28.3 bits (60), Expect = 4.0 Identities = 16/32 (50%), Positives = 18/32 (56%), Gaps = 5/32 (15%) Frame = -2 Query: 443 CG-CD---HLAGNVCGCDWHGNGRL-HDGQGN 363 CG CD AG V CDW G+G L D +GN Sbjct: 667 CGPCDVAVDSAGRVIACDWSGDGVLVFDSRGN 698 >SB_3680| Best HMM Match : Keratin_B2 (HMM E-Value=0.0024) Length = 180 Score = 27.9 bits (59), Expect = 5.3 Identities = 8/23 (34%), Positives = 13/23 (56%) Frame = +1 Query: 403 SHPHTLPARWSHPHTLTAXWSHP 471 +HP + + W HP+ A W +P Sbjct: 127 NHPIKIQSNWCHPNKTQANWCYP 149 Score = 27.9 bits (59), Expect = 5.3 Identities = 9/21 (42%), Positives = 11/21 (52%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSH 468 HP A W+HP A W+H Sbjct: 158 HPTKTHANWNHPSKTRANWNH 178 Score = 27.5 bits (58), Expect = 6.9 Identities = 8/22 (36%), Positives = 11/22 (50%) Frame = +1 Query: 406 HPHTLPARWSHPHTLTAXWSHP 471 HP+ A W H + + W HP Sbjct: 38 HPYKTQANWCHTSKIQSNWYHP 59 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,103,980 Number of Sequences: 59808 Number of extensions: 151813 Number of successful extensions: 486 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 315 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 483 length of database: 16,821,457 effective HSP length: 77 effective length of database: 12,216,241 effective search space used: 1148326654 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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