Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31650
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    71   1e-13
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    71   1e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    54   1e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    31   0.10 
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   3.8  
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe...    25   5.1  
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb...    25   6.7  
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    25   6.7  

>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 44/136 (32%), Positives = 71/136 (52%)
 Frame = +3

Query: 54  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 233
           AFS++D  G G+I   ++GDLLRA   NPTLA I                  FL + ++ 
Sbjct: 11  AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67

Query: 234 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 413
                 G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K       
Sbjct: 68  NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124

Query: 414 DDGMIPYAAFLKKVMA 461
            DGM+ Y  F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 70.5 bits (165), Expect = 1e-13
 Identities = 47/149 (31%), Positives = 74/149 (49%), Gaps = 3/149 (2%)
 Frame = +3

Query: 18  TSAXNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXX 191
           T    D + A F  AFS++D +  G I +  LG ++R+L  +PT A +            
Sbjct: 3   TRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGN 62

Query: 192 XXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDD 368
                  FL + ++  KD D    E+  E  K++DK+ NG +   ELTH L +LGE+L  
Sbjct: 63  GTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQ 120

Query: 369 SEVAEVTKDCMDPEDDDGMIPYAAFLKKV 455
            EVA++ ++     D DG+I Y  F + +
Sbjct: 121 EEVADMIREA--DTDGDGVINYEEFSRVI 147


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 35/136 (25%), Positives = 68/136 (50%)
 Frame = +3

Query: 54  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 233
           AF +YD +  G I   ++G +LR+L  N T A +                  F+     +
Sbjct: 14  AFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFV-----S 68

Query: 234 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 413
            K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP  
Sbjct: 69  NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-T 126

Query: 414 DDGMIPYAAFLKKVMA 461
           + G   Y  F++++MA
Sbjct: 127 NSGSFDYYDFVQRIMA 142



 Score = 30.7 bits (66), Expect = 0.14
 Identities = 14/60 (23%), Positives = 34/60 (56%)
 Frame = +3

Query: 261 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 440
           ++  E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
 Frame = +3

Query: 54  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 230
           AF ++D +    ID   L   +RAL  N   + +                   F+ + ++
Sbjct: 42  AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101

Query: 231 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 410
              ++D    E+     +L+D +E G +    L      L E +DD E+  + ++     
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157

Query: 411 DDDGMIPYAAFLKKVM 458
           D DG I    F+  +M
Sbjct: 158 DQDGEINEQEFIAIMM 173



 Score = 29.5 bits (63), Expect = 0.31
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = +3

Query: 222 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 395
           Y+  + +  +   +D  E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 24  YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81



 Score = 25.4 bits (53), Expect = 5.1
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +3

Query: 36  VERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 137
           +E    AF ++D +  GKI   NL  + + LN N
Sbjct: 109 LEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +3

Query: 348 LGEKLDDSEVAEVTKDCMDPED 413
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 551

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
 Frame = +1

Query: 31  TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 204
           T      +PS    L+A  + S+P+ +L  S E +      + +R  ++  ++  S +  
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368

Query: 205 KSSFPSTAKQRKTKT 249
           KSSFPST K +   T
Sbjct: 369 KSSFPSTIKDQPNLT 383


>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 490

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 19/78 (24%), Positives = 38/78 (48%)
 Frame = +1

Query: 22  QQXTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSH 201
           ++ ++L  +L+P    T KAK  S+ S    S   S+ +  W     + V++  A   S 
Sbjct: 345 REPSSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SP 401

Query: 202 SKSSFPSTAKQRKTKTRE 255
           S++S P+ +K  +   ++
Sbjct: 402 SEASLPNLSKYSRKNAKK 419


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
 Frame = +3

Query: 33  DVER-ASFAFSIYDFEGKGKID 95
           DV R  SF F +YDF G G +D
Sbjct: 612 DVMRNISFIFELYDFNGDGFMD 633


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,873,808
Number of Sequences: 5004
Number of extensions: 33861
Number of successful extensions: 115
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)