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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31643
         (468 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...    59   4e-08
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    57   2e-07
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...    57   2e-07
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...    57   2e-07
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L...    56   5e-07
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:...    42   0.005
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ...    41   0.016
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba...    35   1.0  
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba...    34   1.8  
UniRef50_Q4P3P6 Cluster: Putative uncharacterized protein; n=1; ...    33   2.4  
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la...    33   2.4  
UniRef50_Q54GW2 Cluster: Putative uncharacterized protein; n=1; ...    32   5.4  

>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score = 59.3 bits (137), Expect = 4e-08
 Identities = 26/28 (92%), Positives = 26/28 (92%)
 Frame = +2

Query: 383 NSLAVVLXRTDWENPGVTQLNRLAAHPP 466
           NSLAVVL R DWENPGVTQLNRLAAHPP
Sbjct: 20  NSLAVVLQRRDWENPGVTQLNRLAAHPP 47


>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 57.2 bits (132), Expect = 2e-07
 Identities = 24/27 (88%), Positives = 25/27 (92%)
 Frame = +3

Query: 387 HWPSFYXVLTGKTLALPNXIALQHIPL 467
           HWPSFY V+TGKTLALPN IALQHIPL
Sbjct: 5   HWPSFYNVVTGKTLALPNLIALQHIPL 31


>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score = 57.2 bits (132), Expect = 2e-07
 Identities = 25/29 (86%), Positives = 25/29 (86%)
 Frame = +2

Query: 380 YNSLAVVLXRTDWENPGVTQLNRLAAHPP 466
           Y  LAVVL R DWENPGVTQLNRLAAHPP
Sbjct: 65  YYGLAVVLQRRDWENPGVTQLNRLAAHPP 93


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score = 57.2 bits (132), Expect = 2e-07
 Identities = 25/28 (89%), Positives = 26/28 (92%)
 Frame = +2

Query: 383 NSLAVVLXRTDWENPGVTQLNRLAAHPP 466
           +SLAVVL R DWENPGVTQLNRLAAHPP
Sbjct: 6   DSLAVVLQRRDWENPGVTQLNRLAAHPP 33


>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
           protein - Phage M13mp18
          Length = 102

 Score = 55.6 bits (128), Expect = 5e-07
 Identities = 24/27 (88%), Positives = 25/27 (92%)
 Frame = +2

Query: 386 SLAVVLXRTDWENPGVTQLNRLAAHPP 466
           +LAVVL R DWENPGVTQLNRLAAHPP
Sbjct: 25  ALAVVLQRRDWENPGVTQLNRLAAHPP 51


>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
           Beta-galactosidase - Yersinia pseudotuberculosis
          Length = 1066

 Score = 42.3 bits (95), Expect = 0.005
 Identities = 17/27 (62%), Positives = 20/27 (74%)
 Frame = +2

Query: 386 SLAVVLXRTDWENPGVTQLNRLAAHPP 466
           SL  +L R DWENP +TQ +RL AHPP
Sbjct: 14  SLPQILSRRDWENPQITQYHRLEAHPP 40


>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
           Enterobacter sakazakii ATCC BAA-894|Rep: Putative
           uncharacterized protein - Enterobacter sakazakii ATCC
           BAA-894
          Length = 1043

 Score = 40.7 bits (91), Expect = 0.016
 Identities = 15/34 (44%), Positives = 21/34 (61%)
 Frame = +2

Query: 365 YXEXXYNSLAVVLXRTDWENPGVTQLNRLAAHPP 466
           + E     LA +L R DW+NP +T +NRL +H P
Sbjct: 10  FNELQTRPLATILARNDWQNPAITSVNRLPSHTP 43


>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
           Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
           pneumoniae
          Length = 1034

 Score = 34.7 bits (76), Expect = 1.0
 Identities = 14/22 (63%), Positives = 15/22 (68%)
 Frame = +2

Query: 398 VLXRTDWENPGVTQLNRLAAHP 463
           VL R DW N  +T LNRL AHP
Sbjct: 17  VLAREDWHNQTITHLNRLPAHP 38


>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
           Gammaproteobacteria|Rep: Beta-galactosidase -
           Pseudoalteromonas haloplanktis (Alteromonas
           haloplanktis)
          Length = 1039

 Score = 33.9 bits (74), Expect = 1.8
 Identities = 13/27 (48%), Positives = 18/27 (66%)
 Frame = +2

Query: 386 SLAVVLXRTDWENPGVTQLNRLAAHPP 466
           SL  ++ R DWENP   Q+N++ AH P
Sbjct: 3   SLQHIINRRDWENPITVQVNQVKAHSP 29


>UniRef50_Q4P3P6 Cluster: Putative uncharacterized protein; n=1;
           Ustilago maydis|Rep: Putative uncharacterized protein -
           Ustilago maydis (Smut fungus)
          Length = 215

 Score = 33.5 bits (73), Expect = 2.4
 Identities = 18/54 (33%), Positives = 30/54 (55%)
 Frame = -2

Query: 233 ALRVVL*HPVXIYXHILFLFFSLXACYLIRXISXLKTNRDKDRETEREHALQTY 72
           +  VVL   V I   + FL+ S  A YL++ +  L+    + R ++R+HA +TY
Sbjct: 149 SFHVVLFLAVVINTALSFLYESFLAKYLVKFVKALQRFSRRSRRSKRKHANKTY 202


>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
           lactis|Rep: Beta-galactosidase - Lactococcus lactis
           subsp. lactis (Streptococcus lactis)
          Length = 998

 Score = 33.5 bits (73), Expect = 2.4
 Identities = 14/23 (60%), Positives = 15/23 (65%)
 Frame = +2

Query: 398 VLXRTDWENPGVTQLNRLAAHPP 466
           VL R DWENP V+  NRL  H P
Sbjct: 9   VLERKDWENPVVSNWNRLPMHTP 31


>UniRef50_Q54GW2 Cluster: Putative uncharacterized protein; n=1;
           Dictyostelium discoideum AX4|Rep: Putative
           uncharacterized protein - Dictyostelium discoideum AX4
          Length = 1059

 Score = 32.3 bits (70), Expect = 5.4
 Identities = 17/70 (24%), Positives = 36/70 (51%)
 Frame = -2

Query: 311 GMLYSVTTVLKYCEWNCYKGPFNLLSALRVVL*HPVXIYXHILFLFFSLXACYLIRXISX 132
           G LY ++ +LK+CE+N  K        +  V   P+ +   +L+ F       +++ I+ 
Sbjct: 418 GWLYIISAILKFCEFNVLK------EIIESVPDFPLKLISKLLYSFDLNCPSQMVKLINL 471

Query: 131 LKTNRDKDRE 102
           LK+ +++D +
Sbjct: 472 LKSKQEEDND 481


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 359,898,474
Number of Sequences: 1657284
Number of extensions: 5024287
Number of successful extensions: 6868
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6688
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6866
length of database: 575,637,011
effective HSP length: 94
effective length of database: 419,852,315
effective search space used: 25610991215
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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