BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31640 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 272 3e-74 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 142 3e-35 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 132 4e-32 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 106 2e-24 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 80 2e-16 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 71 8e-14 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 68 1e-12 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 6e-10 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 40 3e-04 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.7 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.1 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 6.7 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 6.7 SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 8.9 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 272 bits (666), Expect = 3e-74 Identities = 126/145 (86%), Positives = 135/145 (93%) Frame = +1 Query: 82 REKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC 261 +EKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ Sbjct: 213 KEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENA 272 Query: 262 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 441 GIHE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+AP Sbjct: 273 GIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAP 332 Query: 442 PERKYSVWIGGSILASLSTFQQMWI 516 PERKYSVWIGGSILASLSTFQQMWI Sbjct: 333 PERKYSVWIGGSILASLSTFQQMWI 357 Score = 50.8 bits (116), Expect = 1e-07 Identities = 23/27 (85%), Positives = 25/27 (92%) Frame = +3 Query: 3 DYLMKILTXRGYSFTTTAEREIVRDIK 83 DYLMKIL RGY+F+TTAEREIVRDIK Sbjct: 187 DYLMKILMERGYTFSTTAEREIVRDIK 213 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 142 bits (344), Expect = 3e-35 Identities = 64/145 (44%), Positives = 94/145 (64%) Frame = +1 Query: 82 REKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC 261 +EK CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ Sbjct: 218 KEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEAS 277 Query: 262 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 441 G+H ++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ ++KI A Sbjct: 278 GLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYAS 337 Query: 442 PERKYSVWIGGSILASLSTFQQMWI 516 PER ++ W+GGSILASLSTF+++ I Sbjct: 338 PERMHNAWLGGSILASLSTFRRLLI 362 Score = 29.5 bits (63), Expect = 0.31 Identities = 13/27 (48%), Positives = 18/27 (66%) Frame = +3 Query: 3 DYLMKILTXRGYSFTTTAEREIVRDIK 83 DYL L G+ ++AE+EIVR+IK Sbjct: 192 DYLQLQLRKSGHELVSSAEKEIVREIK 218 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 132 bits (318), Expect = 4e-32 Identities = 66/157 (42%), Positives = 101/157 (64%), Gaps = 12/157 (7%) Frame = +1 Query: 82 REKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC 261 +EKLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E Sbjct: 213 KEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQP 272 Query: 262 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL--------APST 417 G+ E ++++I DVDIRK LY V+SGG++MY G+ R++KEI L P+ Sbjct: 273 GLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPAR 332 Query: 418 I---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWI 516 + K+KI P R+++V+IGG++LA ++ MW+ Sbjct: 333 LPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWV 369 Score = 30.7 bits (66), Expect = 0.14 Identities = 12/26 (46%), Positives = 19/26 (73%) Frame = +3 Query: 6 YLMKILTXRGYSFTTTAEREIVRDIK 83 YL+ +L +GY+F TA+ E VR++K Sbjct: 188 YLISLLLRKGYAFNRTADFETVREMK 213 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 106 bits (254), Expect = 2e-24 Identities = 58/139 (41%), Positives = 77/139 (55%), Gaps = 11/139 (7%) Frame = +1 Query: 133 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYN 285 A AS+ K +E PDG G ERFR E LF PSF S G+HE VY Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 286 SIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYS 459 SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S I + + Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNA 390 Query: 460 VWIGGSILASLSTFQQMWI 516 VW GGSILASL FQ +W+ Sbjct: 391 VWFGGSILASLDNFQHLWV 409 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 80.2 bits (189), Expect = 2e-16 Identities = 40/88 (45%), Positives = 57/88 (64%), Gaps = 3/88 (3%) Frame = +1 Query: 262 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 441 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 442 ---PERKYSVWIGGSILASLSTFQQMWI 516 ER Y+ W+GGSIL+SL TF Q+WI Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWI 412 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 71.3 bits (167), Expect = 8e-14 Identities = 44/142 (30%), Positives = 68/142 (47%), Gaps = 20/142 (14%) Frame = +1 Query: 82 REKLCYVALDFEQEMATAAAS--TSLEKSYELPD------------------GQVITIGN 201 +E +CYV+ +F+++M + LE Y LPD QV+ + N Sbjct: 219 KESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSN 278 Query: 202 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 381 ERF PE LF PS + + GI E V S+ +I+ L N V GG +PG R Sbjct: 279 ERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKR 338 Query: 382 MQKEITALAPSTIKIKIIAPPE 447 + E+ +LAP+ ++K+ P + Sbjct: 339 LSSELRSLAPANWEVKVFEPSD 360 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 67.7 bits (158), Expect = 1e-12 Identities = 48/173 (27%), Positives = 78/173 (45%), Gaps = 19/173 (10%) Frame = +1 Query: 40 RSPPPLRGKSFVTSREKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNER 207 R+ P K+ +E+ CYV D +E + L+ + E G TI G ER Sbjct: 227 RNEPDSSLKTAERIKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFER 286 Query: 208 FRCPEALFQPSFLGMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRM 384 F PE F P + + E V N + +D+RK LY N V+SGG+T++ +R+ Sbjct: 287 FLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRL 346 Query: 385 QKEITALAPSTI--------------KIKIIAPPERKYSVWIGGSILASLSTF 501 Q+++ + I + +I+ ++ +VW GGS+LA F Sbjct: 347 QRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 58.4 bits (135), Expect = 6e-10 Identities = 25/92 (27%), Positives = 50/92 (54%) Frame = +1 Query: 202 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 381 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 382 MQKEITALAPSTIKIKIIAPPERKYSVWIGGS 477 +++E+T++ P I + W G S Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGAS 685 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 39.5 bits (88), Expect = 3e-04 Identities = 18/70 (25%), Positives = 36/70 (51%), Gaps = 3/70 (4%) Frame = +1 Query: 316 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 486 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 487 SLSTFQQMWI 516 + ++WI Sbjct: 587 RIRIVSELWI 596 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 1.7 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +1 Query: 388 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 498 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 5.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +3 Query: 267 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 395 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 414 DHQDQDHRSPREEVLRMDRWIHPG 485 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 25.0 bits (52), Expect = 6.7 Identities = 14/49 (28%), Positives = 22/49 (44%), Gaps = 1/49 (2%) Frame = +1 Query: 76 TSREKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 219 T CYV+ +E + + SL SY +PD + I + R+ P Sbjct: 33 TEHGVFCYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81 >SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 24.6 bits (51), Expect = 8.9 Identities = 22/93 (23%), Positives = 37/93 (39%), Gaps = 1/93 (1%) Frame = +1 Query: 184 VITIGNERFRCPEALFQPSFLGMESC-GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTM 360 ++T+ NE + E L S S E+ + CD+D ++ + + T + Sbjct: 136 LVTLPNENNQVIEPLSSQSCKSQLSTQNYSESDFGWNQLCDLD---PIFKSLAFTDDTNL 192 Query: 361 YPGIADRMQKEITALAPSTIKIKIIAPPERKYS 459 +P AD I ++K A RKYS Sbjct: 193 FPAFADSEAALIMLKKREMTRVKHRAGRPRKYS 225 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -2 Query: 455 YFLSGGAMILILMVEGARAVISFCILSAIPGY 360 Y +SGGA + +A +S CI +PGY Sbjct: 412 YCISGGAAL----AASTQAFLSSCICPVLPGY 439 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,881,757 Number of Sequences: 5004 Number of extensions: 34462 Number of successful extensions: 131 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 126 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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