BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31621 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 306 1e-84 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 149 3e-37 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 140 1e-34 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 121 6e-29 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 1e-20 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 79 3e-16 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 74 1e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 71 1e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 2e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 0.95 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.7 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 5.1 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.1 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 6.7 SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 8.9 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 306 bits (752), Expect = 1e-84 Identities = 141/163 (86%), Positives = 152/163 (93%) Frame = +3 Query: 9 RXKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC 188 + KLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ Sbjct: 213 KEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENA 272 Query: 189 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 368 GIHE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+AP Sbjct: 273 GIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAP 332 Query: 369 PERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 497 PERKYSVWIGGSILASLSTFQQMWISK+EYDESGPGIV+RKCF Sbjct: 333 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 149 bits (360), Expect = 3e-37 Identities = 68/163 (41%), Positives = 104/163 (63%) Frame = +3 Query: 9 RXKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC 188 + K CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ Sbjct: 218 KEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEAS 277 Query: 189 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 368 G+H ++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ ++KI A Sbjct: 278 GLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYAS 337 Query: 369 PERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 497 PER ++ W+GGSILASLSTF+++ I+ EEY ++ ++ R+ F Sbjct: 338 PERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 140 bits (338), Expect = 1e-34 Identities = 70/166 (42%), Positives = 107/166 (64%), Gaps = 12/166 (7%) Frame = +3 Query: 9 RXKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC 188 + KLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E Sbjct: 213 KEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQP 272 Query: 189 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL--------APST 344 G+ E ++++I DVDIRK LY V+SGG++MY G+ R++KEI L P+ Sbjct: 273 GLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPAR 332 Query: 345 I---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKEEYDESG 470 + K+KI P R+++V+IGG++LA ++ MW+SK E++E G Sbjct: 333 LPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 121 bits (292), Expect = 6e-29 Identities = 65/148 (43%), Positives = 85/148 (57%), Gaps = 11/148 (7%) Frame = +3 Query: 60 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYN 212 A AS+ K +E PDG G ERFR E LF PSF S G+HE VY Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 213 SIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYS 386 SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S I + + Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNA 390 Query: 387 VWIGGSILASLSTFQQMWISKEEYDESG 470 VW GGSILASL FQ +W+SK+EYDE G Sbjct: 391 VWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.9 bits (223), Expect = 1e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = +3 Query: 189 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 368 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 369 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 494 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 79.4 bits (187), Expect = 3e-16 Identities = 48/162 (29%), Positives = 77/162 (47%) Frame = +3 Query: 6 SRXKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMES 185 S+ ++CY D+ +T + + + QV+ + NERF PE LF PS + + Sbjct: 242 SKLEICYALPDY----STGKHGYIVRDINQKIEQQVLNLSNERFMIPELLFSPSDIEIRE 297 Query: 186 CGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIA 365 GI E V S+ +I+ L N V GG +PG R+ E+ +LAP+ ++K+ Sbjct: 298 AGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFE 357 Query: 366 PPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRK 491 P + W S + L + I++ EY E G I+ RK Sbjct: 358 PSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 74.1 bits (174), Expect = 1e-14 Identities = 50/179 (27%), Positives = 81/179 (45%), Gaps = 19/179 (10%) Frame = +3 Query: 9 RXKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFLG 176 + + CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 241 KEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIAS 300 Query: 177 MESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI-- 347 + + E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 301 SDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHR 360 Query: 348 ------------KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHR 488 + +I+ ++ +VW GGS+LA F +K +Y+E G I R Sbjct: 361 SEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.9 bits (166), Expect = 1e-13 Identities = 32/115 (27%), Positives = 62/115 (53%) Frame = +3 Query: 129 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 308 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 309 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 473 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 43.2 bits (97), Expect = 2e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = +3 Query: 243 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 413 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 414 SLSTFQQMWISKEEYDESGPGIVHRK 491 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 0.95 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +3 Query: 390 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 491 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 1.7 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +3 Query: 315 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 425 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 25.4 bits (53), Expect = 5.1 Identities = 14/49 (28%), Positives = 22/49 (44%), Gaps = 1/49 (2%) Frame = +3 Query: 3 TSRXKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 146 T CYV+ +E + + SL SY +PD + I + R+ P Sbjct: 33 TEHGVFCYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 5.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +2 Query: 194 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 322 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +2 Query: 341 DHQDQDHRSPREEVLRMDRWIHPG 412 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 24.6 bits (51), Expect = 8.9 Identities = 22/93 (23%), Positives = 37/93 (39%), Gaps = 1/93 (1%) Frame = +3 Query: 111 VITIGNERFRCPEALFQPSFLGMESC-GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTM 287 ++T+ NE + E L S S E+ + CD+D ++ + + T + Sbjct: 136 LVTLPNENNQVIEPLSSQSCKSQLSTQNYSESDFGWNQLCDLD---PIFKSLAFTDDTNL 192 Query: 288 YPGIADRMQKEITALAPSTIKIKIIAPPERKYS 386 +P AD I ++K A RKYS Sbjct: 193 FPAFADSEAALIMLKKREMTRVKHRAGRPRKYS 225 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -3 Query: 382 YFLSGGAMILILMVEGARAVISFCILSAIPGY 287 Y +SGGA + +A +S CI +PGY Sbjct: 412 YCISGGAAL----AASTQAFLSSCICPVLPGY 439 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,718,103 Number of Sequences: 5004 Number of extensions: 33577 Number of successful extensions: 129 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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