Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31590
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 35 0.028
At5g61390.1 68418.m07702 exonuclease family protein contains exo... 33 0.15
At5g07710.1 68418.m00884 exonuclease family protein contains exo... 31 0.35
At2g47470.2 68415.m05924 thioredoxin family protein similar to p... 29 1.4
At2g47470.1 68415.m05925 thioredoxin family protein similar to p... 29 1.4
At5g22350.1 68418.m02607 expressed protein 29 2.5
At3g05480.1 68416.m00600 cell cycle checkpoint control protein f... 29 2.5
At2g26380.1 68415.m03166 disease resistance protein-related / LR... 29 2.5
At1g33590.1 68414.m04158 disease resistance protein-related / LR... 29 2.5
At2g16870.1 68415.m01941 disease resistance protein (TIR-NBS-LRR... 28 4.3
At5g44530.1 68418.m05455 subtilase family protein contains Pfam ... 27 5.7
At1g62340.1 68414.m07034 subtilisin-like serine protease / abnor... 27 5.7
At3g50920.1 68416.m05575 phosphatidic acid phosphatase-related /... 27 7.5
At5g18890.1 68418.m02244 inosine-uridine preferring nucleoside h... 27 9.9
At4g21650.1 68417.m03137 subtilase family protein contains Pfam ... 27 9.9
At4g21640.1 68417.m03136 subtilase family protein similar to sub... 27 9.9
At3g06880.1 68416.m00817 transducin family protein / WD-40 repea... 27 9.9
At3g06570.1 68416.m00763 kelch repeat-containing F-box family pr... 27 9.9
At2g32970.1 68415.m04041 expressed protein 27 9.9
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains
Pfam profiles PF00632: HECT-domain
(ubiquitin-transferase), PF00612: IQ calmodulin-binding
motif
Length = 1142
Score = 35.1 bits (77), Expect = 0.028
Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = +1
Query: 238 TETEMNKETCLQVNSTQDQLQDIQSVLPDMTDFKYIGTETMQDADTAKWQMVQPVGD-KL 414
TE ++E V L I VL D +K I E +DA+TAK ++Q +G K
Sbjct: 165 TECNYSQERIKDVIGVNALLLRILIVLTDPKSWKIITNENFEDAETAKKIIIQFIGSCKS 224
Query: 415 NKYTMWVKYKKTL 453
Y+ +Y KTL
Sbjct: 225 GYYSAVRRYIKTL 237
>At5g61390.1 68418.m07702 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 487
Score = 32.7 bits (71), Expect = 0.15
Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +1
Query: 19 VDKGSPPQWSP-VYTVKGLLNIPYAELHEP 105
VD GS W+P V +KG LN P A +H P
Sbjct: 383 VDSGSGSDWNPLVIPMKGFLNCPTARIHIP 412
>At5g07710.1 68418.m00884 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 468
Score = 31.5 bits (68), Expect = 0.35
Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = +1
Query: 19 VDKGSPPQWSPVYT-VKGLLNIPYAELH 99
VD GS +W+PV +KG +N P A +H
Sbjct: 365 VDSGSTSEWNPVVNPMKGFVNYPNARIH 392
>At2g47470.2 68415.m05924 thioredoxin family protein similar to
protein disulfide isomerase [Dictyostelium discoideum]
GI:2627440; contains Pfam profile: PF00085 Thioredoxin
Length = 266
Score = 29.5 bits (63), Expect = 1.4
Identities = 15/25 (60%), Positives = 19/25 (76%), Gaps = 3/25 (12%)
Frame = +3
Query: 186 VSGLPTLRYFYKD-RAGH--NGNRD 251
VSG PTL++F KD +AGH +G RD
Sbjct: 213 VSGFPTLKFFPKDNKAGHDYDGGRD 237
>At2g47470.1 68415.m05925 thioredoxin family protein similar to
protein disulfide isomerase [Dictyostelium discoideum]
GI:2627440; contains Pfam profile: PF00085 Thioredoxin
Length = 361
Score = 29.5 bits (63), Expect = 1.4
Identities = 15/25 (60%), Positives = 19/25 (76%), Gaps = 3/25 (12%)
Frame = +3
Query: 186 VSGLPTLRYFYKD-RAGH--NGNRD 251
VSG PTL++F KD +AGH +G RD
Sbjct: 213 VSGFPTLKFFPKDNKAGHDYDGGRD 237
>At5g22350.1 68418.m02607 expressed protein
Length = 427
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +1
Query: 4 FASGYVDKGSPPQWSPVYTVKGLLNIPYAELHEPFYAWYD 123
F ++ PPQ V T L I YA L + AW+D
Sbjct: 210 FMRSWITPREPPQDHVVLTTGALHQIDYASLRKAASAWHD 249
>At3g05480.1 68416.m00600 cell cycle checkpoint control protein
family contains Pfam profile PF04139: Rad9; contains
Prosite PS00976: Myristoyl-CoA:protein
N-myristoyltransferase signature 2; similar to
radio-resistance/chemo-resistance/cell cycle checkpoint
control protein (GI:3869274) [Mus musculus]
Length = 439
Score = 28.7 bits (61), Expect = 2.5
Identities = 32/141 (22%), Positives = 52/141 (36%), Gaps = 9/141 (6%)
Frame = +1
Query: 118 YDSKNSKSRIDYYGGMVKTYQFTSAVYPP-YGTSIKIAPVTTETEMNKETCLQ-VNSTQD 291
+D+ K + Y GM KTY T V P S+ + M+ + + + Q
Sbjct: 98 HDASKVKWTLQCYSGMKKTYWITCNVEPDIQHLSLDRGRFPSTLVMHPRNLSKLLGNFQS 157
Query: 292 QLQDIQSVLPDMTDFKYIGTETMQDADTAKWQMVQPV--GDKLNKYTMWVK-----YKKT 450
LQ+I + D T F + V P GD L +W+ + T
Sbjct: 158 SLQEITIIATDQTSFPSDAASEIGGKAVEFRSYVDPTKDGDSLLHTQLWIDPSEEFLQYT 217
Query: 451 LKGDSVPIPVRYEMKGFNSLL 513
GD P+ + + +K + L
Sbjct: 218 HAGD--PVDITFSLKELKAFL 236
>At2g26380.1 68415.m03166 disease resistance protein-related / LRR
protein-related contains leucine rich-repeat domains
Pfam:PF00560, INTERPRO:IPR001611; similar to Hcr2-2A
[Lycopersicon pimpinellifolium] gi|3894389|gb|AAC78594
Length = 480
Score = 28.7 bits (61), Expect = 2.5
Identities = 24/86 (27%), Positives = 32/86 (37%), Gaps = 6/86 (6%)
Frame = -2
Query: 455 FKVFLYLTHIVYLFSLSPTGCTICH------LAVSASCIVSVPIYLKSVMSGSTDWMSWS 294
F F T +++L L+PT CH L S I P + S TD SW+
Sbjct: 6 FHNFFIFTAVIFLRCLNPTAAATCHPDDEAGLLAFKSGITKDPSGILSTWKKGTDCCSWN 65
Query: 293 *SCVELTCRHVSLFISVSVVTGAIFI 216
R V L I + IF+
Sbjct: 66 GVSCPNGNRVVVLTIRIESDDAGIFL 91
>At1g33590.1 68414.m04158 disease resistance protein-related / LRR
protein-related contains leucine rich-repeat domains
Pfam:PF00560, INTERPRO:IPR001611; similar to Hcr2-5D
[Lycopersicon esculentum] gi|3894393|gb|AAC78596
Length = 477
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -2
Query: 461 SPFKVFLYLTHIVYLFSLSPTGCTICH 381
S F +F++ T +++L L+PTG CH
Sbjct: 3 SSFTLFIF-TFVIFLQCLNPTGAATCH 28
>At2g16870.1 68415.m01941 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1109
Score = 27.9 bits (59), Expect = 4.3
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +2
Query: 86 MLNCMSHSMLGTTVRTANQESITMEVWSRLTSSRQRSTHPTVLL 217
M NC+S ++ T + A+ E ITM SRL + ST+ LL
Sbjct: 677 MANCISLEVIPTHINLASLEHITMTGCSRLKTFPDFSTNIERLL 720
>At5g44530.1 68418.m05455 subtilase family protein contains Pfam
profiles: PF00082 subtilase family
Length = 840
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 199 PPYGTSIKIAPVTTETEMNKETCLQVNST 285
PPYG S+K++P M + L V T
Sbjct: 776 PPYGVSMKVSPTQFSIAMGENQVLSVTLT 804
>At1g62340.1 68414.m07034 subtilisin-like serine protease / abnormal
leaf shape1 (ALE1) identical to subtilisin-like serine
protease [Arabidopsis thaliana] GI:16444944
Length = 832
Score = 27.5 bits (58), Expect = 5.7
Identities = 12/42 (28%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +1
Query: 169 KTYQFTSAVYPPYGTSIKIAPV--TTETEMNKETCLQVNSTQ 288
KT + +V PP GT++++ P T + ++ ++ N TQ
Sbjct: 762 KTETYLGSVLPPNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQ 803
>At3g50920.1 68416.m05575 phosphatidic acid phosphatase-related /
PAP2-related contains Pfam profile PF01569: PAP2
superfamily
Length = 279
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/59 (25%), Positives = 25/59 (42%)
Frame = -1
Query: 213 STVGWVDR*RELVSLDHTSIVIDS*FAVLTVVPSIEWLMQFSIGYI*ESLYCVYWTPLW 37
S + W+ + L + + S F L V + Q +G I SL+C+ W +W
Sbjct: 179 SVMEWLGTNGVSLFLSGLILALGSYFIRLRVSQKLHTSSQVVVGAIVGSLFCILWYTMW 237
>At5g18890.1 68418.m02244 inosine-uridine preferring nucleoside
hydrolase family protein contains Pfam profile PF01156:
Inosine-uridine preferring nucleoside hydrolase
Length = 550
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 92 NCMSHSMLGTTVRTANQESITMEVWSRLTSSRQRSTHPTVL 214
N ++H T R ++ + +EVW LT S + TVL
Sbjct: 280 NSVAHGAPSDTDRPELRQPLALEVWQNLTKSVDEVSKITVL 320
>At4g21650.1 68417.m03137 subtilase family protein contains Pfam
domain, PF00082: Subtilase family; contains Pfam domain,
PF02225: protease associated (PA) domain
Length = 766
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 2/50 (4%)
Frame = +1
Query: 25 KGSPPQWSPVYTVKGLLNIPY--AELHEPFYAWYDSKNSKSRIDYYGGMV 168
K P+WSP L+ + + EP +A +K DY GG+V
Sbjct: 563 KSLHPKWSPAAVRSALVTTAWRTSPSGEPIFAEGSNKKLADPFDYGGGLV 612
>At4g21640.1 68417.m03136 subtilase family protein similar to
subtilase SP1 [Oryza sativa] GI:9957714
Length = 733
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 2/50 (4%)
Frame = +1
Query: 25 KGSPPQWSPVYTVKGLLNIPY--AELHEPFYAWYDSKNSKSRIDYYGGMV 168
K P+WSP L+ + + EP +A +K DY GG+V
Sbjct: 530 KSLHPKWSPAAMRSALVTTAWRTSPSGEPIFAQGSNKKLADPFDYGGGLV 579
>At3g06880.1 68416.m00817 transducin family protein / WD-40 repeat
family protein similar to PAK/PLC-interacting protein 1
(GI:4211689) {Homo sapiens}
Length = 1115
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = -2
Query: 428 IVYLFSLSPTGCTICHLAVSASCIVSVPIYLKSVMSGSTDWMSWS*SCVEL 276
+ YL S T + + S +VS+ +Y+ V+S W+S C+ L
Sbjct: 1 MAYLSSSQSTFISCSENSSLDSILVSINVYILGVISDPEAWISLKQKCITL 51
>At3g06570.1 68416.m00763 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 390
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = +1
Query: 358 MQDADTAKWQMVQPVGDKLNKYTMWVKYKKTLKGDSV-PIPVRYE 489
+ D D KW+M+ L + T W + K L D V +P YE
Sbjct: 337 LPDNDDYKWKMIWCAEIALERRTSWEIWGKVLWFDPVLTVPADYE 381
>At2g32970.1 68415.m04041 expressed protein
Length = 577
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = +1
Query: 70 LLNIPYAELHEPFYAWYDSKNSKSRIDYYGGMVKTYQFTSAV 195
+L + L P + W ++ + SRI Y G M+ + F AV
Sbjct: 306 VLKATASRLCGPLFDWVETWDMVSRIKYLGTMLFLHNFQKAV 347
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.314 0.132 0.402
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,186,070
Number of Sequences: 28952
Number of extensions: 260237
Number of successful extensions: 525
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 525
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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