BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31575 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 151 4e-37 At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 71 5e-13 At5g63910.1 68418.m08025 expressed protein 33 0.15 At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.61 At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.61 At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-depende... 29 1.9 At5g07800.1 68418.m00894 flavin-containing monooxygenase family ... 27 5.7 At5g13390.1 68418.m01542 expressed protein 27 9.9 >At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+] / GPDH strong similarity to SP|P52425 Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8) {Cuphea lanceolata}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 400 Score = 151 bits (365), Expect = 4e-37 Identities = 72/136 (52%), Positives = 97/136 (71%), Gaps = 1/136 (0%) Frame = +3 Query: 111 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINETHENV 287 K+KV +VGSGNWGS AK++ NA L +F D V MWV+EE++ G+KL ++IN+T+ENV Sbjct: 54 KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENV 113 Query: 288 KYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKG 467 KYLPG KL NVVA PD+ A KDA++L+FV PHQF+ IC L GKI A+SL+KG Sbjct: 114 KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKG 173 Query: 468 FDIAEGGGIDLISHII 515 ++ + G +IS +I Sbjct: 174 MEVKKEGPC-MISSLI 188 >At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehydrogenase family protein low similarity to SP|Q26756 Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 420 Score = 70.9 bits (166), Expect = 5e-13 Identities = 42/120 (35%), Positives = 59/120 (49%) Frame = +3 Query: 117 KVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVKYL 296 KV ++G G++G+A+A V R L V M V + + + INE H N KY Sbjct: 89 KVVVLGGGSFGTAMAAHVARRKEGLE-----VNMLVRDSFV-----CQSINENHHNCKYF 138 Query: 297 PGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDI 476 P HKLP NV+A D A DAD + VP QF + + + P +SL KG ++ Sbjct: 139 PEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLEL 198 >At5g63910.1 68418.m08025 expressed protein Length = 500 Score = 32.7 bits (71), Expect = 0.15 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%) Frame = +3 Query: 120 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLT-EIINETHE 281 VCIVGSG GS++A + + S + ++ M+ EI+ G+ T + +T E Sbjct: 38 VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGGRMRTVTVAGDTFE 92 >At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 481 Score = 30.7 bits (66), Expect = 0.61 Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 5/80 (6%) Frame = +3 Query: 75 DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKL 254 D +LD ++QP V + G+ G AK + A L + R +WV ++G Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDGSCC 308 Query: 255 TEIINE----THENV-KYLP 299 +E ++ T +N +YLP Sbjct: 309 SEYVSANGGGTEDNTPEYLP 328 >At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 487 Score = 30.7 bits (66), Expect = 0.61 Identities = 20/82 (24%), Positives = 35/82 (42%) Frame = +3 Query: 84 ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEI 263 +LD +KQP V + G+ GS AK + A L + R +WV ++G + Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDGSACSAY 316 Query: 264 INETHENVKYLPGHKLPSNVVA 329 ++ ++ LP V+ Sbjct: 317 LSANSGKIRDGTPDYLPEGFVS 338 >At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-dependent thioredoxin reductase 2 (NTR2) identical to SP|Q39242 Length = 330 Score = 29.1 bits (62), Expect = 1.9 Identities = 26/79 (32%), Positives = 35/79 (44%) Frame = +3 Query: 111 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVK 290 K KVCIVGSG A R FE W+ +I G +LT T +V+ Sbjct: 5 KTKVCIVGSGPAAHTAAIYASRAELKPLLFEG----WMANDIAPGGQLT-----TTTDVE 55 Query: 291 YLPGHKLPSNVVAVPDVVE 347 PG P ++ + D+VE Sbjct: 56 NFPG--FPEGILGI-DIVE 71 >At5g07800.1 68418.m00894 flavin-containing monooxygenase family protein / FMO family protein similar to flavin-containing monooxygenase 2 (FMO2) from Homo sapiens [GI:1834493]; contains Pfam profile: PF00743 Flavin-binding monooxygenase-like Length = 460 Score = 27.5 bits (58), Expect = 5.7 Identities = 15/52 (28%), Positives = 23/52 (44%), Gaps = 3/52 (5%) Frame = +3 Query: 96 ADKQPKNKVCIVGSGNWGSAIAKIV---GRNAASLSNFEDRVTMWVYEEIIE 242 A + KVC++G+G G A+ + G L ED W Y+ +E Sbjct: 8 ASRSRSKKVCVIGAGPAGLVSARELRKEGHKVVVLEQNEDVGGQWFYQPNVE 59 >At5g13390.1 68418.m01542 expressed protein Length = 1123 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = -2 Query: 176 STNNFRNSRTPVSRSNDANF 117 ST NF + TP SRSN++ F Sbjct: 37 STRNFIDRATPTSRSNNSRF 56 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,311,624 Number of Sequences: 28952 Number of extensions: 163439 Number of successful extensions: 606 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 592 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 605 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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