Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31562
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 65 7e-12
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 56 3e-09
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 29 0.55
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 26 2.9
SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy... 25 6.7
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 25 6.7
SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|... 25 6.7
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 6.7
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po... 25 6.7
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 25 8.9
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 64.9 bits (151), Expect = 7e-12
Identities = 36/103 (34%), Positives = 55/103 (53%), Gaps = 1/103 (0%)
Frame = +2
Query: 89 LRRRRKPKXWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGG-QFKM 265
L R + K WIE LG P FE L++G VL L+ K +P + KI + QF+
Sbjct: 41 LCRVDEAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRH 100
Query: 266 MENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 394
+NI F I G+P+I F+ D++E K++ +V+ + AL
Sbjct: 101 SDNINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 56.0 bits (129), Expect = 3e-09
Identities = 38/132 (28%), Positives = 65/132 (49%), Gaps = 1/132 (0%)
Frame = +2
Query: 86 TLRRRRKPKXWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKM 265
T + ++ + WIE L K D L+ G +LC++ + ++ + + F
Sbjct: 2 TSQLEKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQ 60
Query: 266 MENITNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLG 442
MENI+ F A + VP D+FQT DL+E+++ QV+ ++ + R + LG
Sbjct: 61 MENISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLG 120
Query: 443 PKPADECKRDFS 478
PK A++ R FS
Sbjct: 121 PKLAEKKPRVFS 132
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 28.7 bits (61), Expect = 0.55
Identities = 27/99 (27%), Positives = 46/99 (46%), Gaps = 4/99 (4%)
Frame = +2
Query: 110 KXWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQ 289
K W+E ++ + F D L +G VLCQL K P + ++++ E T +
Sbjct: 71 KKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTVYL 127
Query: 290 SA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 394
+A + G+ F+T DL + +I +V+ L AL
Sbjct: 128 NAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 26.2 bits (55), Expect = 2.9
Identities = 22/64 (34%), Positives = 28/64 (43%), Gaps = 7/64 (10%)
Frame = -1
Query: 222 PGFSLLMSWQR----TVPSLRTSSNSSPGG--NLA-PRTPSIHXXXXXXXXXXXLGSDLS 64
PG L QR T P L TSS++ PGG N+A T S+ + +S
Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSSAYHPSGSSNGIS 269
Query: 63 ANLT 52
A LT
Sbjct: 270 AGLT 273
>SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 922
Score = 25.0 bits (52), Expect = 6.7
Identities = 19/72 (26%), Positives = 31/72 (43%), Gaps = 7/72 (9%)
Frame = +2
Query: 23 SHNHTTCLWNVKFALRSLPSATLR------RRRKPKXWIEGVLGAKF-PPGELFEDVLKD 181
S +HT LW++K + +P T + + + + + VL K P G L D
Sbjct: 496 SADHTVKLWSLKSSFDFVPGTTRKVTTLKLEQTRQIDFTDDVLAVKISPDGRFVAASLLD 555
Query: 182 GTVLCQLINKLK 217
TV ++ LK
Sbjct: 556 NTVKVYYLDSLK 567
>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 589
Score = 25.0 bits (52), Expect = 6.7
Identities = 10/45 (22%), Positives = 20/45 (44%)
Frame = +1
Query: 229 PQDQHHRWTVQNDGEHHKLPVCNQSIRCTRHRCVPNRRSMGEKRH 363
P+ +++++ +HHKL + RC P +K+H
Sbjct: 206 PRSSTTSYSIRDADKHHKLTTSGVTKMNITERCKPKTTIQTDKKH 250
>SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 376
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = -3
Query: 466 FAFVSGLGSEAGAAPFRVSVSLTTEGKQCADYLGDVFFL 350
FAFVS EA + F ++ G Q + + +FF+
Sbjct: 51 FAFVSEKSLEAASVKFVATIIAERMGAQFSSFYDKIFFV 89
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 25.0 bits (52), Expect = 6.7
Identities = 25/87 (28%), Positives = 39/87 (44%), Gaps = 10/87 (11%)
Frame = +2
Query: 131 LGAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV-------PKINTTGGQFKMMENIT 280
+G++ P E FE D KDG +L +LIN P ++ + N FK +EN
Sbjct: 132 VGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNN 191
Query: 281 NFQSAIKAYGVPDIDVFQTVDLWEKKD 361
++ KA G I D+ E ++
Sbjct: 192 VVINSAKAMGGISITNIGAGDILEGRE 218
>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1159
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -3
Query: 454 SGLGSEAGAAPFRVSVSLTTEGKQ 383
SG G+ AG++PF S +LT E K+
Sbjct: 656 SGFGNTAGSSPF--SFNLTKESKE 677
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 24.6 bits (51), Expect = 8.9
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 515 DRSQFARPSGLHQKSHVCIRQRAWV 441
DR +F RP H+K +RQR+ V
Sbjct: 153 DRVEFKRPKNPHEKHFRPLRQRSSV 177
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,268,686
Number of Sequences: 5004
Number of extensions: 49416
Number of successful extensions: 130
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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