Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31562
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09170.1 68414.m01024 kinesin motor protein-related similar t... 49 2e-06
At5g27000.1 68418.m03221 kinesin motor protein-related non-conse... 48 3e-06
At2g47500.1 68415.m05929 kinesin motor protein-related 47 7e-06
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 44 6e-05
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina... 42 3e-04
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina... 42 3e-04
At5g35700.1 68418.m04269 fimbrin-like protein, putative similar ... 39 0.002
At5g41310.1 68418.m05020 kinesin motor protein-related 36 0.016
At5g48460.1 68418.m05992 fimbrin-like protein, putative strong s... 35 0.037
At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 34 0.065
At2g04750.1 68415.m00485 fimbrin-like protein, putative similar ... 34 0.065
At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical t... 33 0.11
At5g55400.1 68418.m06902 fimbrin-like protein, putative similar ... 33 0.15
At5g04980.1 68418.m00527 endonuclease/exonuclease/phosphatase fa... 29 1.9
At3g49210.1 68416.m05378 expressed protein 28 3.2
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 28 4.3
At5g14570.1 68418.m01708 transporter, putative similar to trans-... 28 4.3
At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-... 27 5.7
At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family prote... 27 5.7
At3g52930.1 68416.m05834 fructose-bisphosphate aldolase, putativ... 27 9.9
At3g44630.3 68416.m04798 disease resistance protein RPP1-WsB-lik... 27 9.9
At3g44630.2 68416.m04800 disease resistance protein RPP1-WsB-lik... 27 9.9
At3g44630.1 68416.m04799 disease resistance protein RPP1-WsB-lik... 27 9.9
>At1g09170.1 68414.m01024 kinesin motor protein-related similar to
GB:AAB61066
Length = 1010
Score = 49.2 bits (112), Expect = 2e-06
Identities = 42/144 (29%), Positives = 70/144 (48%), Gaps = 20/144 (13%)
Frame = +2
Query: 59 FALRSLP-SATLRRRRKPKXWIE---GVLGAKFPPGELFED----VLKDGTVLCQLINKL 214
F R LP + RR + W+ GV+G + P + E+ L+ G +LC ++N++
Sbjct: 43 FGGRDLPVDPSDLRRYEAARWVRNTLGVVGGRDLPADPSEEDFRIALRSGILLCNVLNRV 102
Query: 215 KPGSVPK---------INTTG---GQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKK 358
KPG+VPK +N G F+ EN+ NF ++ G+P F+ D +
Sbjct: 103 KPGAVPKVVEAPNDPLVNQDGAALSAFQYFENLRNFLVFVEEMGIP---TFEVSDFEKGG 159
Query: 359 DIAQVVSTLFALGRETYRHAEWSG 430
A++V + AL ++YR + SG
Sbjct: 160 KSARIVECVLAL--KSYREWKQSG 181
>At5g27000.1 68418.m03221 kinesin motor protein-related
non-consensus AT donor splice site at exon 12;
non-consensus AC acceptor splice site at exon 13
Length = 987
Score = 48.4 bits (110), Expect = 3e-06
Identities = 41/137 (29%), Positives = 68/137 (49%), Gaps = 16/137 (11%)
Frame = +2
Query: 32 HTTCLWNVKFALRSLPSATLRRRRKPKXWIEGVLGA---KFPPGELFEDV----LKDGTV 190
H++ +V R + ++LRR + W+ ++G K PGE E+ L+ G V
Sbjct: 26 HSSRSSDVGLVSRKVEESSLRRY-EAAGWLRDMIGVSNGKDFPGEPSEEEFRLGLRSGIV 84
Query: 191 LCQLINKLKPGSVPKI-----NTTGG----QFKMMENITNFQSAIKAYGVPDIDVFQTVD 343
LC ++NK+ PGSV K+ + G F+ ENI NF AI+ G+P F+ D
Sbjct: 85 LCNVLNKVNPGSVSKVVEAPDDVADGAALSAFQYFENIRNFLVAIEEMGLPS---FEASD 141
Query: 344 LWEKKDIAQVVSTLFAL 394
+ + ++V+ + AL
Sbjct: 142 MEKGGKSIRIVNCILAL 158
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 47.2 bits (107), Expect = 7e-06
Identities = 36/119 (30%), Positives = 60/119 (50%), Gaps = 19/119 (15%)
Frame = +2
Query: 95 RRRKPKXWIE---GVLGAKFPPGELFEDVLK----DGTVLCQLINKLKPGSVPKI----- 238
RR + W+ GV+GAK P E E+ L+ G +LC+++NK++PG+V K+
Sbjct: 46 RRYEAANWLRRMVGVVGAKDLPAEPTEEGLRLGLRSGIILCKVLNKVQPGAVSKVVESPC 105
Query: 239 -------NTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 394
F+ EN+ NF AI+ G P F+ DL + + ++VV+ + A+
Sbjct: 106 DAILVADGAPLSAFQYFENVRNFLVAIQEMGFP---TFEASDLEQGGNASRVVNCVLAI 161
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 44.0 bits (99), Expect = 6e-05
Identities = 36/135 (26%), Positives = 61/135 (45%), Gaps = 15/135 (11%)
Frame = +2
Query: 35 TTCLWNVKFALRSLPSATLRRRRKPKXWIEGVLGA----KFPPGELFEDVLKDGTVLCQL 202
TT L A R A RR + + W++ V+G P + F L++G +LC
Sbjct: 2 TTGLHEFNLASRRAEEAAARRFQAVQ-WLKSVVGQLGIPNQPSEKEFISCLRNGMILCNA 60
Query: 203 INKLKPGSVPKI----NTTGGQFKM------MENITNFQSAIKAYGVPDIDVFQ-TVDLW 349
INK+ PG+V K+ + G++++ EN+ NF A++ +P + D
Sbjct: 61 INKIHPGAVSKVVENYSYLNGEYQLPPAYQYFENVRNFLVALETLRLPGFEASDLEKDNL 120
Query: 350 EKKDIAQVVSTLFAL 394
E + +VV + L
Sbjct: 121 ESGSVTKVVDCILGL 135
>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1065
Score = 41.5 bits (93), Expect = 3e-04
Identities = 34/98 (34%), Positives = 49/98 (50%), Gaps = 5/98 (5%)
Frame = +2
Query: 116 WIEGVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPKINTTGGQFKMME-NIT 280
W+ L P E ED L+DGTVLC L+N+L PGS+ GG F+ I
Sbjct: 48 WLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSM----RMGGSFEPASVKIE 103
Query: 281 NFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 394
F +A+ +P F+ D+ E+ D+ V+ +L AL
Sbjct: 104 RFLTAMDEMALPR---FEVSDI-EQGDMVPVLQSLKAL 137
>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1064
Score = 41.5 bits (93), Expect = 3e-04
Identities = 34/98 (34%), Positives = 49/98 (50%), Gaps = 5/98 (5%)
Frame = +2
Query: 116 WIEGVLGAKFPPGELFED----VLKDGTVLCQLINKLKPGSVPKINTTGGQFKMME-NIT 280
W+ L P E ED L+DGTVLC L+N+L PGS+ GG F+ I
Sbjct: 48 WLNETLPYLKLPWEASEDELRACLRDGTVLCSLLNQLSPGSM----RMGGSFEPASVKIE 103
Query: 281 NFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 394
F +A+ +P F+ D+ E+ D+ V+ +L AL
Sbjct: 104 RFLTAMDEMALPR---FEVSDI-EQGDMVPVLQSLKAL 137
>At5g35700.1 68418.m04269 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893, fimbrin [Schizosaccharomyces pombe]
GI:3057144; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 687
Score = 39.1 bits (87), Expect = 0.002
Identities = 23/65 (35%), Positives = 38/65 (58%), Gaps = 4/65 (6%)
Frame = +2
Query: 146 PPGELFEDVLKDGTVLCQLINKLKPGSVPK--INT--TGGQFKMMENITNFQSAIKAYGV 313
P F D++KDG +LC+LIN PG++ + INT T ++ EN+T ++ KA G
Sbjct: 150 PATNAFFDLVKDGVLLCKLINVAVPGTIDERAINTKKTLNPWERNENLTLGLNSAKAIGC 209
Query: 314 PDIDV 328
+++
Sbjct: 210 TVVNI 214
>At5g41310.1 68418.m05020 kinesin motor protein-related
Length = 961
Score = 35.9 bits (79), Expect = 0.016
Identities = 26/72 (36%), Positives = 36/72 (50%), Gaps = 5/72 (6%)
Frame = +2
Query: 116 WIEGVLGAKFPPGELFEDVLK----DGTVLCQLINKLKPGSVPKINTTGGQFKM-MENIT 280
W+ L P E E+ L+ DGTVLC L+N+L PGS+ GG F+ NI
Sbjct: 49 WLNETLPYLNLPWEASEEELRACLVDGTVLCNLLNQLSPGSM----RMGGSFEPGCVNIE 104
Query: 281 NFQSAIKAYGVP 316
F +A+ +P
Sbjct: 105 RFLAAMDEMTLP 116
>At5g48460.1 68418.m05992 fimbrin-like protein, putative strong
similarity to fimbrin-like protein AtFim2 [Arabidopsis
thaliana] GI:2737926; contains Pfam profile PF00307:
Calponin homology (CH) domain
Length = 654
Score = 34.7 bits (76), Expect = 0.037
Identities = 23/62 (37%), Positives = 36/62 (58%), Gaps = 4/62 (6%)
Frame = +2
Query: 155 ELFEDVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDI 322
+LFE V KDG +LC+LIN PG++ + INT ++ EN T ++ KA G +
Sbjct: 156 DLFE-VAKDGVLLCKLINVAVPGTIDERAINTKSMLNPWERNENHTLCLNSAKAIGCTVV 214
Query: 323 DV 328
++
Sbjct: 215 NI 216
Score = 30.3 bits (65), Expect = 0.80
Identities = 20/72 (27%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = +2
Query: 17 KHSHNHTTCLWNVKFALRSLPSATLRRRRKP-KXWIEGVLGAKFPPGELFEDVLKDGTVL 193
+H + +T + F + R K + WI G+ + +FED L+DG +L
Sbjct: 369 QHRNGLSTQTKQISFLENLADDIQISREEKAFRFWINSFDGSVYI-NNVFED-LRDGWIL 426
Query: 194 CQLINKLKPGSV 229
Q ++K+ PG V
Sbjct: 427 LQTLDKVSPGIV 438
>At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4
other kinesin-like proteins of A. thaliana: F02P16.12
(PID:g2191180), katA (D11371), katB (D21137), and katC
(D21138); contains non-consensus AT-AC splice sites at
intron 10
Length = 1087
Score = 33.9 bits (74), Expect = 0.065
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +2
Query: 146 PPGELFEDVLKDGTVLCQLINKLKPGSVPKI 238
P + F L++G +LC ++NK+ PGSV K+
Sbjct: 13 PSEDEFSLALRNGLILCNVLNKVNPGSVLKV 43
>At2g04750.1 68415.m00485 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 652
Score = 33.9 bits (74), Expect = 0.065
Identities = 19/58 (32%), Positives = 35/58 (60%), Gaps = 4/58 (6%)
Frame = +2
Query: 167 DVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDIDV 328
D++KDG +LC+LIN PG++ + INT ++ EN++ ++ KA G +++
Sbjct: 151 DLVKDGVLLCKLINIAVPGTIDERAINTKKELNPWERTENLSLCLNSAKAIGCTVVNI 208
>At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893
Length = 687
Score = 33.1 bits (72), Expect = 0.11
Identities = 25/70 (35%), Positives = 41/70 (58%), Gaps = 4/70 (5%)
Frame = +2
Query: 155 ELFEDVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDI 322
+L+E ++KDG +LC+LIN PG++ + INT ++ EN T ++ KA G +
Sbjct: 155 QLYE-LVKDGVLLCKLINVAVPGTIDERAINTKRVLNPWERNENHTLCLNSAKAVGCSVV 213
Query: 323 DVFQTVDLWE 352
++ T DL E
Sbjct: 214 NI-GTQDLAE 222
>At5g55400.1 68418.m06902 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 714
Score = 32.7 bits (71), Expect = 0.15
Identities = 25/70 (35%), Positives = 41/70 (58%), Gaps = 4/70 (5%)
Frame = +2
Query: 155 ELFEDVLKDGTVLCQLINKLKPGSVPK--INTTG--GQFKMMENITNFQSAIKAYGVPDI 322
+L+E ++KDG +LC+LIN PG++ + INT ++ EN T ++ KA G +
Sbjct: 156 DLYE-LVKDGVLLCKLINIAVPGTIDERAINTKRVLNPWERNENHTLCLNSAKAVGCSVV 214
Query: 323 DVFQTVDLWE 352
++ T DL E
Sbjct: 215 NI-GTQDLAE 223
>At5g04980.1 68418.m00527 endonuclease/exonuclease/phosphatase
family protein contains similarity to inositol
polyphosphate 5-phosphatase I (GI:10444261) and II
(GI:10444263) [Arabidopsis thaliana]; contains Pfam
profile PF03372: Endonuclease/Exonuclease/phosphatase
family
Length = 437
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/42 (40%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -1
Query: 243 VLILG-TEPGFSLLMSWQRTVPSLRTSSNSSPGGNLAPRTPS 121
VL+LG EP W + S+SS GG L+P+TPS
Sbjct: 57 VLVLGDNEPA----AKWLAMINQSLNKSSSSSGGRLSPKTPS 94
>At3g49210.1 68416.m05378 expressed protein
Length = 518
Score = 28.3 bits (60), Expect = 3.2
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Frame = -1
Query: 363 MSFFSHRSTVWNTSMSGTPYALIADWKFVM--FSIILNCPPVVLILGTEPGFSLLMSWQR 190
+SF+ H T S+ G P+AL ++ M +I L P V+ ++P LL W++
Sbjct: 445 ISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTVI---SDP-HRLLDDWEK 500
Query: 189 TVPSLRTS 166
++ S++ +
Sbjct: 501 SLQSIKAA 508
>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profiles PF00307: Calponin homology (CH)
domain, PF03271: EB1 protein
Length = 329
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 3/34 (8%)
Frame = +2
Query: 182 GTVLCQLINKLKPGSVP--KIN-TTGGQFKMMEN 274
G V CQL++ + PG+VP K+N +++M++N
Sbjct: 40 GAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQN 73
>At5g14570.1 68418.m01708 transporter, putative similar to
trans-membrane nitrate transporter protein AtNRT2:1
[Arabidopsis thaliana] GI:3747058, high-affinity nitrate
transporter ACH1 [Arabidopsis thaliana] GI:3608362;
contains Pfam profile PF00083: Sugar (and other)
transporter
Length = 493
Score = 27.9 bits (59), Expect = 4.3
Identities = 26/104 (25%), Positives = 48/104 (46%), Gaps = 2/104 (1%)
Frame = +2
Query: 185 TVLCQLINKLKPGSVPKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDI 364
+V Q + L G VP ++T ++ IT + A V +F D+ +++ I
Sbjct: 375 SVFVQAASGLVFGVVPFVSTRS--LGVVAGITGSGGTVGAV-VTQFLLFSGDDVRKQRSI 431
Query: 365 AQV--VSTLFALGRETYRHAEWSGPCLGPKPADECKRDFSDEVL 490
+ + ++ +FAL + +W G C GP + E + D S +L
Sbjct: 432 SLMGLMTFVFALSVTSIYFPQWGGMCCGPSSSSE-EEDISRGLL 474
>At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-1,
Zea mays, EMBL:X58573
Length = 235
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 407 YRHAEWSGPCLGPKPADECKRDFSDEVLKAGQTVI 511
Y + GP L P+ D KR+F DE+LK T +
Sbjct: 99 YLDNTFEGPSLYPE--DHAKREFGDELLKYTDTFV 131
>At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family protein
low similarity to hydrolases from Rhodococcus sp. EtbD2
GI:3273241, EtbD1 GI:3273239; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 314
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2
Query: 173 LKDGTVLCQLINKLKPGSVPKIN 241
LKDGTV+ ++K KP S PK N
Sbjct: 31 LKDGTVVNFWVSKTKPESKPKPN 53
>At3g52930.1 68416.m05834 fructose-bisphosphate aldolase, putative
similar to SP|O65735|ALF_CICAR Fructose-bisphosphate
aldolase, cytoplasmic isozyme {Cicer arietinum},
cytosolic aldolase [Fragaria x ananassa] GI:10645188;
contains Pfam profile PF00274 Fructose-bisphosphate
aldolase class-I
Length = 358
Score = 26.6 bits (56), Expect = 9.9
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +2
Query: 122 EGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTG 250
E L K G+LF D+LK+G VL + K+ G+V T G
Sbjct: 76 EETLYQKSSDGKLFVDILKEGGVLPGI--KVDKGTVELAGTDG 116
>At3g44630.3 68416.m04798 disease resistance protein RPP1-WsB-like
(TIR-NBS-LRR class), putative domain signature
TIR-NBS-LRR exists, suggestive of a disease resistance
protein., closest Col-0 homolog to RPP1-WsB
Length = 1240
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -1
Query: 198 WQRTVPSLRTSSNSSPGG 145
W+RT+P LRTS + GG
Sbjct: 484 WERTLPRLRTSLDGKIGG 501
>At3g44630.2 68416.m04800 disease resistance protein RPP1-WsB-like
(TIR-NBS-LRR class), putative domain signature
TIR-NBS-LRR exists, suggestive of a disease resistance
protein., closest Col-0 homolog to RPP1-WsB
Length = 1214
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -1
Query: 198 WQRTVPSLRTSSNSSPGG 145
W+RT+P LRTS + GG
Sbjct: 484 WERTLPRLRTSLDGKIGG 501
>At3g44630.1 68416.m04799 disease resistance protein RPP1-WsB-like
(TIR-NBS-LRR class), putative domain signature
TIR-NBS-LRR exists, suggestive of a disease resistance
protein., closest Col-0 homolog to RPP1-WsB
Length = 1214
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -1
Query: 198 WQRTVPSLRTSSNSSPGG 145
W+RT+P LRTS + GG
Sbjct: 484 WERTLPRLRTSLDGKIGG 501
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,377,514
Number of Sequences: 28952
Number of extensions: 282752
Number of successful extensions: 851
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 830
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 848
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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