Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31560
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 35 0.028
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 35 0.028
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 32 0.26
At4g28910.2 68417.m04132 expressed protein 31 0.46
At4g28910.1 68417.m04131 expressed protein 31 0.46
At1g75270.1 68414.m08744 dehydroascorbate reductase, putative si... 31 0.46
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 30 0.80
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 30 0.80
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 30 0.80
At5g41220.1 68418.m05009 glutathione S-transferase, putative sim... 30 1.1
At5g17220.1 68418.m02018 glutathione S-transferase, putative 30 1.1
At1g49860.1 68414.m05590 glutathione S-transferase, putative sim... 30 1.1
At4g02520.1 68417.m00345 glutathione S-transferase, putative 29 1.4
At2g02930.1 68415.m00241 glutathione S-transferase, putative 29 1.4
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 29 1.4
At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 29 1.9
At3g03190.1 68416.m00315 glutathione S-transferase, putative ide... 29 2.5
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 29 2.5
At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 28 4.3
At1g19570.1 68414.m02437 dehydroascorbate reductase, putative si... 28 4.3
At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 27 5.7
At5g44800.1 68418.m05492 chromodomain-helicase-DNA-binding famil... 27 7.5
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 27 7.5
At2g36690.1 68415.m04501 oxidoreductase, 2OG-Fe(II) oxygenase fa... 27 7.5
At4g02650.1 68417.m00360 epsin N-terminal homology (ENTH) domain... 27 9.9
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 35.1 bits (77), Expect = 0.028
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 5/56 (8%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKY-----GLAGANDEEAFEIDQ 277
P++ PFG++PVLV + +S AI RY+ KY L G EE +++Q
Sbjct: 42 PEYLAIQPFGKIPVLVDGDYKIFESRAIMRYIAEKYRSQGPDLLGKTIEERGQVEQ 97
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 35.1 bits (77), Expect = 0.028
Identities = 21/58 (36%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKYG---LAGANDEEAFEIDQNVEF 289
P+F P G++PVL +S AI RY+ RK G L G++ E I+Q ++F
Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDF 100
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 31.9 bits (69), Expect = 0.26
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKYG---LAGANDEEAFEIDQNVEF 289
P F P G++PVL +S AI RY+ R G L G++ E +I+Q ++F
Sbjct: 43 PAFLKMNPIGKVPVLETPEGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDF 100
>At4g28910.2 68417.m04132 expressed protein
Length = 425
Score = 31.1 bits (67), Expect = 0.46
Identities = 17/52 (32%), Positives = 30/52 (57%)
Frame = +2
Query: 224 RKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYEADEELKAKKHEDFSKN 379
RK+G G ND++ E D + +H+ + KA+ V DE A ++ED +++
Sbjct: 116 RKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTA-ENEDVAES 166
>At4g28910.1 68417.m04131 expressed protein
Length = 425
Score = 31.1 bits (67), Expect = 0.46
Identities = 17/52 (32%), Positives = 30/52 (57%)
Frame = +2
Query: 224 RKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYEADEELKAKKHEDFSKN 379
RK+G G ND++ E D + +H+ + KA+ V DE A ++ED +++
Sbjct: 116 RKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTA-ENEDVAES 166
>At1g75270.1 68414.m08744 dehydroascorbate reductase, putative
similar to GI:6939839 from [Oryza sativa]
Length = 213
Score = 31.1 bits (67), Expect = 0.46
Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +2
Query: 92 FEDHRVLSADWPD-FKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKY 232
++ H + +D P F +P G++PV+ +DGK A S I L KY
Sbjct: 37 YKTHLINVSDKPQWFLDISPEGKVPVVKLDGKWVADSDVIVGLLEEKY 84
>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 191
Score = 30.3 bits (65), Expect = 0.80
Identities = 23/61 (37%), Positives = 27/61 (44%), Gaps = 4/61 (6%)
Frame = +2
Query: 62 RMLLAYGGQDFE--DHRVLSADW--PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRK 229
R+ LA G D+E +L D DFK P G +P LV S AI YL K
Sbjct: 24 RIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPALVDGDVVINDSFAIIMYLDEK 83
Query: 230 Y 232
Y
Sbjct: 84 Y 84
>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 221
Score = 30.3 bits (65), Expect = 0.80
Identities = 23/61 (37%), Positives = 27/61 (44%), Gaps = 4/61 (6%)
Frame = +2
Query: 62 RMLLAYGGQDFE--DHRVLSADW--PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRK 229
R+ LA G D+E +L D DFK P G +P LV S AI YL K
Sbjct: 24 RIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPALVDGDVVINDSFAIIMYLDEK 83
Query: 230 Y 232
Y
Sbjct: 84 Y 84
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 30.3 bits (65), Expect = 0.80
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYL 220
P F PFGQ+PV + G + +S AI Y+
Sbjct: 79 PSFLAINPFGQVPVFLDGGLKLTESRAISEYI 110
>At5g41220.1 68418.m05009 glutathione S-transferase, putative
similar to emb|CAA10662
Length = 590
Score = 29.9 bits (64), Expect = 1.1
Identities = 15/37 (40%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGK-QYAQSTAICRYLGRKY 232
P+FK P G++P ++DGK + ++S AI YL Y
Sbjct: 43 PEFKDINPMGKVPA-IVDGKLKLSESHAILIYLSSAY 78
>At5g17220.1 68418.m02018 glutathione S-transferase, putative
Length = 214
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAGAN 250
P+ + PFGQ+P + + +S AI RY K+ G N
Sbjct: 43 PEHLLRQPFGQVPAIEDGDFKLFESRAIARYYATKFADQGTN 84
>At1g49860.1 68414.m05590 glutathione S-transferase, putative
similar to GI:860955 from [Hyoscyamus muticus] (Plant
Physiol. 109 (1), 253-260 (1995))
Length = 254
Score = 29.9 bits (64), Expect = 1.1
Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 8/64 (12%)
Frame = +2
Query: 83 GQDFEDHRVLSADW--PDFKPKT------PFGQMPVLVIDGKQYAQSTAICRYLGRKYGL 238
G DFE ++ DW + K KT PFG++PVL + + AI RYL +Y
Sbjct: 27 GLDFE---LVFVDWLAGEAKTKTFLSTLNPFGEVPVLEDGDLKLFEPKAITRYLAEQYKD 83
Query: 239 AGAN 250
G N
Sbjct: 84 VGTN 87
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +2
Query: 131 FKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAGAN 250
F + PFGQ+P + +S AI +Y+ +Y G N
Sbjct: 46 FLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTN 85
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +2
Query: 131 FKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAGAN 250
F + PFGQ+P + +S AI +Y+ +Y G N
Sbjct: 46 FLSRNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTN 85
>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 228
Score = 29.5 bits (63), Expect = 1.4
Identities = 19/50 (38%), Positives = 21/50 (42%)
Frame = +2
Query: 83 GQDFEDHRVLSADWPDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKY 232
G F+ V D DFK P G +P LV S AI YL KY
Sbjct: 44 GDQFDS--VYRFDLQDFKKINPMGTVPALVDGDVVINDSFAIIMYLDEKY 91
>At3g62760.1 68416.m07050 glutathione S-transferase, putative
Glutathione transferase III(b) - Zea mays, EMBL:AJ010296
Length = 219
Score = 29.1 bits (62), Expect = 1.9
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKY 232
P F PFG++P L D +S AI Y+ K+
Sbjct: 43 PSFLSMNPFGKVPALQDDDLTLFESRAITAYIAEKH 78
>At3g03190.1 68416.m00315 glutathione S-transferase, putative
identical to glutathione S-transferase GB:AAB09584 from
[Arabidopsis thaliana]
Length = 214
Score = 28.7 bits (61), Expect = 2.5
Identities = 26/84 (30%), Positives = 35/84 (41%), Gaps = 6/84 (7%)
Frame = +2
Query: 17 VVYHYFACKALGESGRMLLAYGGQDFE------DHRVLSADWPDFKPKTPFGQMPVLVID 178
VV Y KA R+LL + +D E D L P + PFGQ+P +
Sbjct: 2 VVKVYGQIKAANPQ-RVLLCFLEKDIEFEVIHVDLDKLEQKKPQHLLRQPFGQVPAIEDG 60
Query: 179 GKQYAQSTAICRYLGRKYGLAGAN 250
+ +S AI RY KY G +
Sbjct: 61 YLKLFESRAIARYYATKYADQGTD 84
>At2g30860.1 68415.m03761 glutathione S-transferase, putative
identical to GB:Y12295
Length = 215
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAG 244
P + PFG +P +V + +S A+ RY+ KY G
Sbjct: 42 PAYLALQPFGTVPAVVDGDYKIFESRAVMRYVAEKYRSQG 81
>At2g02380.1 68415.m00176 glutathione S-transferase, putative
similar to gi:167970 gb:AAA72320 gb:AY052332
Length = 223
Score = 27.9 bits (59), Expect = 4.3
Identities = 22/61 (36%), Positives = 26/61 (42%), Gaps = 4/61 (6%)
Frame = +2
Query: 62 RMLLAYGGQDFE--DHRVLSADWPD--FKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRK 229
R+ L G D+E +L D D FK P G +P LV S AI YL K
Sbjct: 27 RIALTLKGLDYEYIPVNLLKGDQSDSDFKKINPMGTVPALVDGDVVINDSFAIIMYLDDK 86
Query: 230 Y 232
Y
Sbjct: 87 Y 87
>At1g19570.1 68414.m02437 dehydroascorbate reductase, putative
similar to GB:BAA90672 from (Oryza sativa)
Length = 213
Score = 27.9 bits (59), Expect = 4.3
Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 92 FEDHRVLSADWPD-FKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKY 232
++ H + +D P F +P G++PVL ID K S I L KY
Sbjct: 37 YKIHLINLSDKPQWFLDISPQGKVPVLKIDDKWVTDSDVIVGILEEKY 84
>At5g41240.1 68418.m05011 glutathione S-transferase, putative
similar to glutathione S-transferase, GST 10b
GB:CAA10662 [Arabidopsis thaliana] 37349.
Length = 591
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 125 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGRKY 232
P+FK P G++P +V + +S AI YL Y
Sbjct: 43 PEFKEINPMGKVPAIVDGRLKLFESHAILIYLSSAY 78
>At5g44800.1 68418.m05492 chromodomain-helicase-DNA-binding family
protein / CHD family protein similar to chromatin
remodeling factor CHD3 (PICKLE) [Arabidopsis thaliana]
GI:6478518; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00628: PHD-finger, PF00385: 'chromo'
(CHRromatin Organization MOdifier)
Length = 2228
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 278 NVEFLHDIRAKAAAVYYEADEELKAKKHEDFSKNVYPDMLKKLN 409
++EFLHD+R KA+A LK E ++ M K L+
Sbjct: 977 SLEFLHDMRIKASAKLTLLHSMLKVLHKEGHRVLIFSQMTKLLD 1020
>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
identical to GB:X95295. Based on identical cDNA hits,
the translation is now 40 AAs longer at the N-terminal,
and start of exon2 is also corrected.
Length = 263
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +2
Query: 146 PFGQMPVLVIDGKQYAQSTAICRYLGRKYGLAG 244
PFGQ+P L +S AI +YL +Y G
Sbjct: 99 PFGQIPALEDGDLTLFESRAITQYLAEEYSEKG 131
>At2g36690.1 68415.m04501 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to IDS3 [Hordeum
vulgare][GI:4514655], leucoanthocyanidin dioxygenase
[SP|P51091][Malus domestica]; contains PF03171
2OG-Fe(II) oxygenase superfamily domain
Length = 366
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = +1
Query: 349 SKKTRGLFEERIP*YVEEVELDS*SEQKVTLLPES*HGVISCSPAC 486
+K+T+ +FE + +E +E+D E L S V++C P C
Sbjct: 182 AKETKEMFEMMVKAILESLEIDGSDEAAKELEEGSQVVVVNCYPPC 227
>At4g02650.1 68417.m00360 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to Chain A,
Calm-N N-Terminal Domain Of Clathrin Assembly Lymphoid
Myeloid Leukaemia Protein, Pi(4,5)p2 Complex
(GP:13399999) {Homo sapiens}; supporting cDNA
gi|26451912|dbj|AK118440.1|
Length = 611
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2
Query: 224 RKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYEADEELKAKK 358
+K G G D ++ E D + +DIR+KA V + E+K +K
Sbjct: 163 KKSGGGGGGDGDSGEEDDHRGTSNDIRSKAIVVKSKPVAEMKTEK 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,192,930
Number of Sequences: 28952
Number of extensions: 160378
Number of successful extensions: 472
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 467
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 472
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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