BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31554 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 36 0.004 SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 35 0.006 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 31 0.14 SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.18 SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.24 SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 0.55 SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 0.72 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 0.95 SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 0.95 SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 1.3 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 2.2 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 2.2 SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 2.2 SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 26 2.9 SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 26 2.9 SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 2.9 SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 3.8 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 3.8 SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 5.1 SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 25 5.1 SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit... 25 6.7 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 6.7 SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 25 6.7 SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|ch... 25 6.7 SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 6.7 SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 6.7 SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 25 8.9 SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 25 8.9 >SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 35.9 bits (79), Expect = 0.004 Identities = 32/99 (32%), Positives = 52/99 (52%), Gaps = 4/99 (4%) Frame = +2 Query: 134 IEHHTKEFHKTLEQQFNSLTKSKDAQDF---SKAWKDGSESVLQQLNAFAKSLQGALGDA 304 I+ K LEQ+ +L ++++A++ ++ + D S S +L A AK A DA Sbjct: 98 IQPDEKTLQDLLEQRQVALREAREAEEELQRARQYNDRSTSEALELEARAKK---AAQDA 154 Query: 305 NGKAKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 418 A E +++ +IER+A K A + E+ ATALRE Sbjct: 155 E-LASERAREAQSSIERSASLREKQAREEAERAATALRE 192 >SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 35.1 bits (77), Expect = 0.006 Identities = 28/107 (26%), Positives = 55/107 (51%), Gaps = 6/107 (5%) Frame = +2 Query: 164 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 334 T+E ++SL KSK + F + ++ + +L N + + L + +A KA+++LEQ Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65 Query: 335 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 466 + + E L K H + E+ + +EKL A ++ + S++L Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 30.7 bits (66), Expect = 0.14 Identities = 30/131 (22%), Positives = 53/131 (40%) Frame = +2 Query: 17 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 196 P S S + A + L + V R F + +EH+ K+LE+Q + L + Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229 Query: 197 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRK 376 SKDA A S+ + S + +L N K++ +L+ ++ R Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVF 288 Query: 377 AHPDVEKNATA 409 P+V + +A Sbjct: 289 HKPEVHLDPSA 299 >SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 30.3 bits (65), Expect = 0.18 Identities = 17/81 (20%), Positives = 38/81 (46%) Frame = +2 Query: 263 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 442 N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + + Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396 Query: 443 TVQESQKLAKKVSSNVQETNE 505 +QE ++ K + Q+ +E Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417 >SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 29.9 bits (64), Expect = 0.24 Identities = 13/46 (28%), Positives = 25/46 (54%) Frame = +2 Query: 230 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 367 KDG+++ +LN FAK L + + +A + + IE+++ E Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156 >SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 28.7 bits (61), Expect = 0.55 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%) Frame = +2 Query: 209 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 364 +D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132 >SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 28.3 bits (60), Expect = 0.72 Identities = 24/88 (27%), Positives = 45/88 (51%) Frame = +2 Query: 134 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 313 IE K F K ++ NS K+ +A F +G++ ++ + A ++L L + Sbjct: 73 IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125 Query: 314 AKEALEQSRQNIERTAEELRKAHPDVEK 397 +EQ+R E T E++++A P++EK Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 27.9 bits (59), Expect = 0.95 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%) Frame = +2 Query: 104 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 274 ++ DFFK + ++ H TL ++ NSL+ +K + G + +LN Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115 Query: 275 KSLQGALGDA 304 + LQG + A Sbjct: 116 R-LQGRMNSA 124 >SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 27.9 bits (59), Expect = 0.95 Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%) Frame = +2 Query: 164 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 325 T++ + SL K D + ++ ES L L L A L D+ KAK Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411 Query: 326 LEQSRQNIERTAEE 367 LEQ+R +ERT E Sbjct: 412 LEQARIELERTQRE 425 >SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 631 Score = 27.5 bits (58), Expect = 1.3 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -2 Query: 341 ASTVPKPPWPCRSRLRALPG 282 AST+ K PWP + L +PG Sbjct: 38 ASTLEKEPWPASTALLVMPG 57 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 26.6 bits (56), Expect = 2.2 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%) Frame = +2 Query: 110 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 229 D +F D++ H K FH E+ + + +K D K W Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 26.6 bits (56), Expect = 2.2 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%) Frame = +2 Query: 278 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 433 S++ L + N + KE +E + RT +E EKN LRE+L+ A Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576 >SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1288 Score = 26.6 bits (56), Expect = 2.2 Identities = 22/74 (29%), Positives = 32/74 (43%) Frame = +2 Query: 239 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 418 S + + A K L GA KAKE ++ R +RTA E+RK +E+ R Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199 Query: 419 KLQAAVQNTVQESQ 460 + A Q + Q Sbjct: 200 ERAAEAQRVAGKEQ 213 >SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 633 Score = 26.2 bits (55), Expect = 2.9 Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 8/75 (10%) Frame = +2 Query: 311 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 466 K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186 Query: 467 AKKVSSNVQETNEKL 511 K + T +KL Sbjct: 187 YKDMEQENASTIQKL 201 >SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 272 Score = 26.2 bits (55), Expect = 2.9 Identities = 15/59 (25%), Positives = 34/59 (57%) Frame = +2 Query: 311 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSN 487 K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+++ + S+ Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-EKISRNLRSS 188 >SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation factor eIF2 beta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 26.2 bits (55), Expect = 2.9 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%) Frame = +2 Query: 146 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 304 +K+ + E+Q +T DFS K + L+AF K L+ + GDA Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142 >SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 394 Score = 25.8 bits (54), Expect = 3.8 Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%) Frame = +2 Query: 74 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 250 C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72 Query: 251 LQQL 262 Q+ Sbjct: 73 NNQI 76 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.8 bits (54), Expect = 3.8 Identities = 18/77 (23%), Positives = 35/77 (45%), Gaps = 3/77 (3%) Frame = +2 Query: 158 HKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANG---KAKEAL 328 H+T+ +Q + +A + ES L N ++ L ++N K +E + Sbjct: 625 HQTITKQLKDTSSKLQQLQLERANFEQKESTLSDENNDLRTKLLKLEESNKSLIKKQEDV 684 Query: 329 EQSRQNIERTAEELRKA 379 + +NI+ E+LRK+ Sbjct: 685 DSLEKNIQTLKEDLRKS 701 >SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 25.4 bits (53), Expect = 5.1 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +2 Query: 368 LRKAHPDVEKNATALREKLQAAVQNTVQE 454 L AH D+E T++RE+L++ + +E Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295 >SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Schizosaccharomyces pombe|chr 3|||Manual Length = 1108 Score = 25.4 bits (53), Expect = 5.1 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = +2 Query: 305 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 400 N K KE E++ + EELR+A PD E++ Sbjct: 22 NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53 >SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit subunit Ker1|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/20 (60%), Positives = 13/20 (65%) Frame = +2 Query: 230 KDGSESVLQQLNAFAKSLQG 289 K GSESVL QLN L+G Sbjct: 40 KSGSESVLSQLNRVLMYLKG 59 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 25.0 bits (52), Expect = 6.7 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -1 Query: 420 FSRRAVAFFSTSGWALRSSSAVRSMF 343 FSR V FFS AL+S S + +MF Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595 >SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +2 Query: 296 GDANGKAKEALEQSRQNIERTAEE 367 G NG+AKE E+ R ++T E+ Sbjct: 473 GKGNGRAKETPEEKRARKKKTKED 496 >SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/38 (26%), Positives = 24/38 (63%) Frame = +1 Query: 7 RISSALSLSTVHHGRQVRSSLRLHRSGPRSDGATRRSR 120 +I ++L T+HHG +V+ ++ ++ G S ++R ++ Sbjct: 122 KIRNSLYSRTIHHGNRVQKKIK-NKHGKNSSKSSRAAQ 158 >SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = -2 Query: 347 CSASTVPKPPWPCRSRLRALPGDS 276 C +T+PKPP+ + L LP S Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKS 313 >SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 25.0 bits (52), Expect = 6.7 Identities = 13/51 (25%), Positives = 26/51 (50%) Frame = +2 Query: 332 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 484 ++RQ E A+ +RK+ PDV + ++ +V + +E + +SS Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334 >SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1115 Score = 24.6 bits (51), Expect = 8.9 Identities = 14/63 (22%), Positives = 33/63 (52%) Frame = +2 Query: 326 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 505 ++ Q+IE T L K D+E++ +++ + V + Q+ ++++ +Q+T E Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553 Query: 506 KLA 514 L+ Sbjct: 554 VLS 556 >SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 786 Score = 24.6 bits (51), Expect = 8.9 Identities = 31/124 (25%), Positives = 50/124 (40%) Frame = +2 Query: 77 IALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQ 256 + L G + D D+ E+ T + + F + +D SK + + + Sbjct: 661 LELLIGGISDVDVEDWKSHTEYRTYIATDPVIKWFWEIIAGWKNEDRSKLLQFATGTSRI 720 Query: 257 QLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAV 436 +N F + LQG+ G ++A + + T R PD T L EKL AV Sbjct: 721 PVNGF-RDLQGSDGPRKFTIEKAGTPDQLPVAHTCFN-RLDLPDYPSKDT-LHEKLSLAV 777 Query: 437 QNTV 448 +NTV Sbjct: 778 ENTV 781 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,579,327 Number of Sequences: 5004 Number of extensions: 26202 Number of successful extensions: 159 Number of sequences better than 10.0: 28 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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