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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31542
         (405 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    57   9e-10
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    57   1e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    40   2e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    26   2.6  
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    25   4.5  

>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 57.2 bits (132), Expect = 9e-10
 Identities = 34/107 (31%), Positives = 54/107 (50%)
 Frame = +3

Query: 78  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257
           AFS++D  G G+I   ++GDLLRA   NPTLA I                     + ++ 
Sbjct: 11  AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67

Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 398
                 G  E+F++  +++DK+  G++   EL + L +LGEKL + E
Sbjct: 68  NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEE 114


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 56.8 bits (131), Expect = 1e-09
 Identities = 34/109 (31%), Positives = 52/109 (47%)
 Frame = +3

Query: 78  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257
           AFS++D +  G I +  LG ++R+L  +PT A +                     +   A
Sbjct: 17  AFSLFDRDQDGNITSNELGVVMRSLGQSPTAAEL-QDMINEVDADGNGTIDFTEFLTMMA 75

Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEXA 404
           +  KD    E+  E  K++DK+ NG +   ELTH L +LGE+L   E A
Sbjct: 76  RKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 39.5 bits (88), Expect = 2e-04
 Identities = 25/107 (23%), Positives = 51/107 (47%)
 Frame = +3

Query: 78  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257
           AF +YD +  G I   ++G +LR+L  N T A +                     + ++ 
Sbjct: 14  AFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAEL--AKLSNELGDAIDEKKFMSFVSNKL 71

Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 398
           +  + +   E++++  +++DK+ +G +  A+    +  LGEKL D+E
Sbjct: 72  RETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNE 115



 Score = 25.0 bits (52), Expect = 4.5
 Identities = 12/40 (30%), Positives = 23/40 (57%)
 Frame = +3

Query: 285 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEXA 404
           ++  E   LYD +++GL+  + +   L +LG  + D+E A
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELA 48


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 25.8 bits (54), Expect = 2.6
 Identities = 21/107 (19%), Positives = 37/107 (34%)
 Frame = +3

Query: 78  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257
           AF ++D +    ID   L   +RAL  N   + +                     +    
Sbjct: 42  AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEV-LKILRDFDKTGKGYLQMEDFVRVMT 100

Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 398
           +   ++   E+     +L+D +E G +    L      L E +DD E
Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQE 147


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 25.0 bits (52), Expect = 4.5
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +3

Query: 60  LKGRSFAFSIYDFEGKGKID 119
           ++  SF F +YDF G G +D
Sbjct: 614 MRNISFIFELYDFNGDGFMD 633


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,414,044
Number of Sequences: 5004
Number of extensions: 22830
Number of successful extensions: 62
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 55
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 60
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 138190552
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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