BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31542 (405 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 57 9e-10 SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 1e-09 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 40 2e-04 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 26 2.6 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 4.5 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 57.2 bits (132), Expect = 9e-10 Identities = 34/107 (31%), Positives = 54/107 (50%) Frame = +3 Query: 78 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257 AFS++D G G+I ++GDLLRA NPTLA I + ++ Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67 Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 398 G E+F++ +++DK+ G++ EL + L +LGEKL + E Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEE 114 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 56.8 bits (131), Expect = 1e-09 Identities = 34/109 (31%), Positives = 52/109 (47%) Frame = +3 Query: 78 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257 AFS++D + G I + LG ++R+L +PT A + + A Sbjct: 17 AFSLFDRDQDGNITSNELGVVMRSLGQSPTAAEL-QDMINEVDADGNGTIDFTEFLTMMA 75 Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEXA 404 + KD E+ E K++DK+ NG + ELTH L +LGE+L E A Sbjct: 76 RKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 39.5 bits (88), Expect = 2e-04 Identities = 25/107 (23%), Positives = 51/107 (47%) Frame = +3 Query: 78 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257 AF +YD + G I ++G +LR+L N T A + + ++ Sbjct: 14 AFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAEL--AKLSNELGDAIDEKKFMSFVSNKL 71 Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 398 + + + E++++ +++DK+ +G + A+ + LGEKL D+E Sbjct: 72 RETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNE 115 Score = 25.0 bits (52), Expect = 4.5 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = +3 Query: 285 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEXA 404 ++ E LYD +++GL+ + + L +LG + D+E A Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELA 48 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 25.8 bits (54), Expect = 2.6 Identities = 21/107 (19%), Positives = 37/107 (34%) Frame = +3 Query: 78 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI*XXXXXXXXXXXXXXXXXXXXIYSQA 257 AF ++D + ID L +RAL N + + + Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEV-LKILRDFDKTGKGYLQMEDFVRVMT 100 Query: 258 KNDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 398 + ++ E+ +L+D +E G + L L E +DD E Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQE 147 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 25.0 bits (52), Expect = 4.5 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +3 Query: 60 LKGRSFAFSIYDFEGKGKID 119 ++ SF F +YDF G G +D Sbjct: 614 MRNISFIFELYDFNGDGFMD 633 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,414,044 Number of Sequences: 5004 Number of extensions: 22830 Number of successful extensions: 62 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 55 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 60 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 138190552 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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