Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31537
(500 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 331 3e-92
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 200 7e-53
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 193 1e-50
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 151 4e-38
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 91 6e-20
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 89 5e-19
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 74 1e-14
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 68 9e-13
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 63 3e-11
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 8e-04
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.17
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.1
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.8
SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosacchar... 25 6.4
SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.4
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 8.4
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 331 bits (814), Expect = 3e-92
Identities = 152/164 (92%), Positives = 160/164 (97%)
Frame = +2
Query: 8 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 187
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 188 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 367
I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181
Query: 368 GRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQ 499
GRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQ
Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQ 225
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 200 bits (489), Expect = 7e-53
Identities = 88/165 (53%), Positives = 124/165 (75%)
Frame = +2
Query: 2 AXRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM 181
A R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM
Sbjct: 60 AVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKM 119
Query: 182 TQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLD 361
+ MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD
Sbjct: 120 CETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLD 179
Query: 362 LAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE 496
+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E
Sbjct: 180 VAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLE 224
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 193 bits (470), Expect = 1e-50
Identities = 87/163 (53%), Positives = 122/163 (74%), Gaps = 1/163 (0%)
Frame = +2
Query: 8 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMT 184
RG+L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+
Sbjct: 66 RGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIA 125
Query: 185 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 364
+ +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++
Sbjct: 126 EYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEI 185
Query: 365 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF 493
GRD+TDYL L + G+ ++AE+EIVR+IKEK CYVA DF
Sbjct: 186 GGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDF 228
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 151 bits (367), Expect = 4e-38
Identities = 79/167 (47%), Positives = 104/167 (62%), Gaps = 10/167 (5%)
Frame = +2
Query: 20 TLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 199
+L YPI HG I NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148
Query: 200 TFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAILR 355
+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY + +I
Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKT 208
Query: 356 LDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALD 490
+ LAGRD+T ++ +L +R S TAER IKE+ CYV D
Sbjct: 209 MPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPD 250
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 91.5 bits (217), Expect = 6e-20
Identities = 47/156 (30%), Positives = 82/156 (52%), Gaps = 2/156 (1%)
Frame = +2
Query: 32 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 211
P E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 212 PAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD 385
P++ I + S Y + + ++GIVL+ G+ SH +P+ G + R+ G +
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSS 204
Query: 386 YLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF 493
YL+K+ + SF + C+V+ D+
Sbjct: 205 YLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDY 240
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 88.6 bits (210), Expect = 5e-19
Identities = 43/134 (32%), Positives = 69/134 (51%)
Frame = +2
Query: 17 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 196
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 197 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 376
E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNA 182
Query: 377 LTDYLMKILTERGY 418
+ D L + L ++ +
Sbjct: 183 INDILAQTLRDKNF 196
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 74.1 bits (174), Expect = 1e-14
Identities = 34/128 (26%), Positives = 66/128 (51%)
Frame = +2
Query: 41 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAM 220
+GII +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 221 YVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKI 400
Y+ QAV +A+ ++T +++D G + P+ +G + I + LAG L + ++
Sbjct: 134 YLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSLAGDFLNANIEQL 193
Query: 401 LTERGYSF 424
F
Sbjct: 194 FNTMNIEF 201
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 67.7 bits (158), Expect = 9e-13
Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%)
Frame = +2
Query: 32 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 199
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 200 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 379
P +A + ++ LYA G G+V+D G + PI +G + A +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231
Query: 380 TDYLMKILTERGYSFTTTAEREIVRDIKE 466
T +L +L E + ++ + DI E
Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 62.9 bits (146), Expect = 3e-11
Identities = 44/170 (25%), Positives = 78/170 (45%), Gaps = 16/170 (9%)
Frame = +2
Query: 38 EHGIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIMFETFNS 211
E G + +W +W N + P ++ +LLT+ + Q++FE F
Sbjct: 64 EKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLVFEEFQF 123
Query: 212 PAMYVAIQAVLSLYASGRTTG--------------IVLDSGDGVSHTVPIYEGYALPHAI 349
A A L + G T +V+DSG +H +P+ + A+
Sbjct: 124 DAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFSVQEQAV 183
Query: 350 LRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQ 499
R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV+ +F++
Sbjct: 184 KRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVSQNFKE 231
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 37.9 bits (84), Expect = 8e-04
Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%)
Frame = +2
Query: 23 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 166
L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L +
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257
Query: 167 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 346
EK+ I+F + + +++ + + +G + V+D G + + EG +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317
Query: 347 ILRLDLAGRDLTDYLMKILTERGYSF 424
++++ G D+T MK+L + +
Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.17
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 92 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 235
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 2.1
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 318 STKVTLCPTPSSVWTW 365
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 2.8
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 134 LLTEAPLNPKANREKMTQIMFETFN 208
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 327
Score = 25.0 bits (52), Expect = 6.4
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +1
Query: 319 LRRLRSAPRHPPSGLGWSRLDRLPHED 399
L++ + +H G WS LDR H+D
Sbjct: 254 LKKSKRVKKHDYGGEDWSLLDRKFHDD 280
>SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 301
Score = 24.6 bits (51), Expect = 8.4
Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%)
Frame = +3
Query: 303 PTPYPSTKVTLC-PTP 347
P P P TKV +C PTP
Sbjct: 256 PAPSPETKVLICGPTP 271
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 24.6 bits (51), Expect = 8.4
Identities = 7/18 (38%), Positives = 13/18 (72%)
Frame = +2
Query: 287 SGDGVSHTVPIYEGYALP 340
+G G+ H + + +GYA+P
Sbjct: 157 AGRGIGHQIMVEQGYAMP 174
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,739,773
Number of Sequences: 5004
Number of extensions: 31917
Number of successful extensions: 137
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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