BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31528 (782 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 268 8e-73 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 136 3e-33 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 123 3e-29 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 118 7e-28 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 2e-20 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 78 2e-15 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 73 5e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 71 2e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 4e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.7 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 3.0 SPAC15E1.03 |rpl36a||60S ribosomal protein L36/L42|Schizosacchar... 27 4.0 SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|... 25 9.3 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 9.3 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 268 bits (656), Expect = 8e-73 Identities = 122/138 (88%), Positives = 130/138 (94%) Frame = +1 Query: 13 KSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANT 192 KSYELPDGQVITIGNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N Sbjct: 238 KSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNV 297 Query: 193 VMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWI 372 VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWI Sbjct: 298 VMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWI 357 Query: 373 SKEEYDESGPGIVHRKCF 426 SK+EYDESGPGIV+RKCF Sbjct: 358 SKQEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 136 bits (330), Expect = 3e-33 Identities = 62/143 (43%), Positives = 96/143 (67%), Gaps = 1/143 (0%) Frame = +1 Query: 1 HLPR-KSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKD 177 H P+ +Y+LPD Q IT+G E F PE LF P +G E+ G+H ++ SI+ D+D+R Sbjct: 238 HKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRST 297 Query: 178 LYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTF 357 LY+N V+SGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF Sbjct: 298 LYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTF 357 Query: 358 QQMWISKEEYDESGPGIVHRKCF 426 +++ I+ EEY ++ ++ R+ F Sbjct: 358 RRLLITSEEY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 123 bits (296), Expect = 3e-29 Identities = 61/141 (43%), Positives = 93/141 (65%), Gaps = 12/141 (8%) Frame = +1 Query: 13 KSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANT 192 ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDIRK LY Sbjct: 238 RNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAI 297 Query: 193 VMSGGTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIGGSIL 339 V+SGG++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++L Sbjct: 298 VLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVL 357 Query: 340 AS-LSTFQQMWISKEEYDESG 399 A ++ MW+SK E++E G Sbjct: 358 ADIMAQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 118 bits (285), Expect = 7e-28 Identities = 62/140 (44%), Positives = 81/140 (57%), Gaps = 11/140 (7%) Frame = +1 Query: 13 KSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYNSIMKCDVD 165 K +E PDG G ERFR E LF PSF S G+HE VY SI+ CD + Sbjct: 279 KPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSE 338 Query: 166 IRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYSVWIGGSIL 339 +R L N V++GGT++ PG+++R+Q E+ LA S I + +VW GGSIL Sbjct: 339 LRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSIL 398 Query: 340 ASLSTFQQMWISKEEYDESG 399 ASL FQ +W+SK+EYDE G Sbjct: 399 ASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.9 bits (223), Expect = 2e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = +1 Query: 118 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 297 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 298 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 423 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 77.8 bits (183), Expect = 2e-15 Identities = 43/128 (33%), Positives = 64/128 (50%) Frame = +1 Query: 37 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTM 216 QV+ + NERF PE LF PS + + GI E V S+ +I+ L N V GG Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331 Query: 217 YPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDES 396 +PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390 Query: 397 GPGIVHRK 420 G I+ RK Sbjct: 391 GANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 72.9 bits (171), Expect = 5e-14 Identities = 41/140 (29%), Positives = 67/140 (47%), Gaps = 15/140 (10%) Frame = +1 Query: 43 ITIGNERFRCPEALFQPSFLGMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMY 219 I +G ERF PE F P + + E V N + +D+RK LY N V+SGG+T++ Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339 Query: 220 PGIADRMQKEITALAPSTI--------------KIKIIAPPERKYSVWIGGSILASLSTF 357 +R+Q+++ + I + +I+ ++ +VW GGS+LA F Sbjct: 340 KNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 Query: 358 QQMWISKEEYDESGPGIVHR 417 +K +Y+E G I R Sbjct: 400 GSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.9 bits (166), Expect = 2e-13 Identities = 32/115 (27%), Positives = 62/115 (53%) Frame = +1 Query: 58 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 237 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 238 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 402 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 43.2 bits (97), Expect = 4e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = +1 Query: 172 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 342 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 343 SLSTFQQMWISKEEYDESGPGIVHRK 420 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.7 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +1 Query: 319 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 420 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 3.0 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +1 Query: 244 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 354 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPAC15E1.03 |rpl36a||60S ribosomal protein L36/L42|Schizosaccharomyces pombe|chr 1|||Manual Length = 106 Score = 26.6 bits (56), Expect = 4.0 Identities = 9/17 (52%), Positives = 11/17 (64%) Frame = +2 Query: 731 MGNVPKQRRTYCKNVKC 781 M N+PK R+TYC C Sbjct: 1 MVNIPKTRKTYCPGKNC 17 >SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = -1 Query: 482 ELGRAQHHTAPGSRGRAA*KHLRWT 408 E+ R++ H+ PGS+G A +L WT Sbjct: 46 EISRSETHSIPGSKGEA---NLIWT 67 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 9.3 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +3 Query: 123 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 251 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,950,516 Number of Sequences: 5004 Number of extensions: 57431 Number of successful extensions: 161 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 379359666 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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