BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31527 (579 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 354 5e-99 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 205 3e-54 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 188 4e-49 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 145 4e-36 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 107 1e-24 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 107 1e-24 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 94 1e-20 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 53 3e-08 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 38 0.001 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.12 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.21 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.5 SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 8.0 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 8.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 354 bits (871), Expect = 5e-99 Identities = 159/174 (91%), Positives = 170/174 (97%) Frame = +2 Query: 56 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 235 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 236 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 415 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 416 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 577 I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAI 175 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 205 bits (501), Expect = 3e-54 Identities = 93/173 (53%), Positives = 127/173 (73%), Gaps = 1/173 (0%) Frame = +2 Query: 62 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 241 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 242 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 418 +L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 419 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 577 +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAI 180 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 188 bits (459), Expect = 4e-49 Identities = 87/172 (50%), Positives = 123/172 (71%), Gaps = 2/172 (1%) Frame = +2 Query: 68 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 241 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 242 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 421 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 422 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 577 FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLV 175 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 145 bits (352), Expect = 4e-36 Identities = 82/200 (41%), Positives = 113/200 (56%), Gaps = 32/200 (16%) Frame = +2 Query: 74 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 202 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 203 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 361 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 362 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 517 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186 Query: 518 GDGVSHTVPIYEGYALPHAI 577 GDGV+H +P+ EGY + +I Sbjct: 187 GDGVTHIIPVAEGYVIGSSI 206 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 107 bits (257), Expect = 1e-24 Identities = 60/187 (32%), Positives = 108/187 (57%), Gaps = 3/187 (1%) Frame = +2 Query: 5 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 184 E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK-- 61 Query: 185 VGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 361 + + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ Sbjct: 62 --LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118 Query: 362 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSH 535 +TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH Sbjct: 119 CMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASH 178 Query: 536 TVPIYEG 556 +P+ G Sbjct: 179 VIPVLNG 185 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 107 bits (257), Expect = 1e-24 Identities = 59/169 (34%), Positives = 91/169 (53%) Frame = +2 Query: 59 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 238 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 239 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 418 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 419 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 565 MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 94.3 bits (224), Expect = 1e-20 Identities = 49/175 (28%), Positives = 94/175 (53%), Gaps = 2/175 (1%) Frame = +2 Query: 59 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 238 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 239 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 412 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 413 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 577 + FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGI 176 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 53.2 bits (122), Expect = 3e-08 Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 4/103 (3%) Frame = +2 Query: 260 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 427 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 428 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 556 P +A + ++ LYA G G+V+D G + PI +G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 37.5 bits (83), Expect = 0.001 Identities = 42/174 (24%), Positives = 70/174 (40%), Gaps = 6/174 (3%) Frame = +2 Query: 74 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 253 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 254 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 421 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 422 FETFNSPAMYVAIQAVLSLYASGRTTGIVLD--SGDGVSHTVPIYEGYALPHAI 577 FE F A A L + G T D +G + I GY+ H I Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHII 171 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.12 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 119 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 262 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.21 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +2 Query: 320 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 463 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.5 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 362 LLTEAPLNPKANREKMTQIMFETFN 436 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Schizosaccharomyces pombe|chr 3|||Manual Length = 791 Score = 25.0 bits (52), Expect = 8.0 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = -1 Query: 315 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 169 WC+ S + SQ+ + +G F PL L WA + + P ITPWW Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 8.0 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 179 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 265 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,264,943 Number of Sequences: 5004 Number of extensions: 45044 Number of successful extensions: 173 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 248115846 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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